Legionella: Methods and Protocols

Front Matter ....Pages i-xxvi
Front Matter ....Pages 1-1
 Analysis of Iron Requirements and Siderophore Production  (Denise M. Burnside, Nicholas P. Cianciotto)....Pages 3-19
 The Pathometabolism of Legionella Studied by Isotopologue Profiling  (Klaus Heuner, Mareike Kunze, Fan Chen, Wolfgang Eisenreich)....Pages 21-44
 Quantification of Viable but Non-Culturable Cells of Legionella pneumophila  (Rosalie S. Braun, Nilmini Mendis, Laam Li, Sebastien P. Faucher)....Pages 45-53
 Selection of Legionella Virulence-Related Traits by Environmental Protozoa  (Francisco Amaro, Howard Shuman)....Pages 55-78
 Migration of Acanthamoeba castellanii Through Legionella Biofilms  (Ramon Hochstrasser, Hubert Hilbi)....Pages 79-89
Front Matter ....Pages 91-91
 Scar-Free Genome Editing in Legionella pneumophila  (Nathalie Bailo, Hussein Kanaan, Elisabeth Kay, Xavier Charpentier, Patricia Doublet, Christophe Gilbert)....Pages 93-105
 Querying Legionella Genomes Using Transposition-Sequencing  (Léo Hardy, Xavier Charpentier)....Pages 107-122
 Screening Targeted Legionella pneumophila Mutant Libraries In Vivo Using INSeq  (Stephanie R. Shames)....Pages 123-144
 Genetic Manipulation of Non-pneumophila Legionella: Protocols Developed for Legionella longbeachae  (Elizabeth Korevaar, Chen Ai Khoo, Hayley J. Newton)....Pages 145-157
Front Matter ....Pages 159-159
 Quantitative Imaging Flow Cytometry of Legionella-Containing Vacuoles in Dually Fluorescence-Labeled Dictyostelium  (Amanda Welin, Stephen Weber, Hubert Hilbi)....Pages 161-177
 Sorting of Phagocytic Cells Infected with Legionella pneumophila  (Monica Rolando, Carmen Buchrieser)....Pages 179-189
 Single Cell Analysis of Legionella and Legionella-Infected Acanthamoeba by Agarose Embedment  (Nicolas Personnic, Bianca Striednig, Hubert Hilbi)....Pages 191-204
 Mitochondrial Dynamics and Activity in Legionella-Infected Cells  (Ok-Ryul Song, Priscille Brodin, Carmen Buchrieser, Pedro Escoll)....Pages 205-220
 Perturbation of Legionella Cell Infection by RNA Interference  (Bernhard Steiner, A. Leoni Swart, Hubert Hilbi)....Pages 221-238
Front Matter ....Pages 239-239
 Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila  (Tomoko Kubori, Hiroki Nagai)....Pages 241-247
 In Situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems Using Electron Cryotomography  (Debnath Ghosal, Mohammed Kaplan, Yi-Wei Chang, Grant J. Jensen)....Pages 249-265
 Methods for Noncanonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins  (Jiazhang Qiu, Zhao-Qing Luo)....Pages 267-276
 Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila  (Nadezhda Levanova, Irina Tabakova, Thomas Jank, Yury Belyi)....Pages 277-287
 Determination of In Vivo Interactomes of Dot/Icm Type IV Secretion System Effectors by Tandem Affinity Purification  (Ernest C. So, Aurélie Mousnier, Gad Frankel, Gunnar N. Schroeder)....Pages 289-303
 Inflammasome Activation in Legionella-Infected Macrophages  (Danielle P. A. Mascarenhas, Dario S. Zamboni)....Pages 305-319
Front Matter ....Pages 321-321
 Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila  (Lina Scheithauer, Michael Steinert)....Pages 323-331
 The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence  (Gad Frankel, Gunnar N. Schroeder)....Pages 333-346
 Dictyostelium Host Response to Legionella Infection: Strategies and Assays  (Salvatore Bozzaro, Simona Buracco, Barbara Peracino, Ludwig Eichinger)....Pages 347-370
 The Caenorhabditis elegans Model of Legionella Infection  (Ann Karen C. Brassinga, Costi D. Sifri)....Pages 371-397
 The Mouse as a Model for Pulmonary Legionella Infection  (Garrett Z. Ng, Anastasia Solomatina, Ian R. van Driel, Elizabeth L. Hartland)....Pages 399-417
Front Matter ....Pages 419-419
 Targeted Metagenomics of Microbial Diversity in Free-Living Amoebae and Water Samples  (Vincent Delafont, Yoann Perrin, Didier Bouchon, Laurent Moulin, Yann Héchard)....Pages 421-428
 Analysis of the Pulmonary Microbiome Composition of Legionella pneumophila-Infected Patients  (Ana Elena Pérez-Cobas, Carmen Buchrieser)....Pages 429-443
 Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry  (Sandra Maaß, Gina Moog, Dörte Becher)....Pages 445-464
Back Matter ....Pages 465-476