Laboratory Methods in Vesicular and Vectorial Transport

Content: 
Series Editor, Page ii
Front Matter, Page iii
Copyright, Page iv
Dedication, Page v
CONTENTS OF METHODS IN CELL BIOLOGY VOLUMES 31, 32, AND 34, Pages xiii-xix
CONTRIBUTORS, Pages xxi-xxiii
PREFACE, Pages xxv-xxviii, ALAN M. TARTAKOFF
Analysis of Protein Topology in the Endoplasmic Reticulum, Pages 3-18, HANS PETER WESSELS, JAMES P. BELTZER, MARTIN SPIESS
Transcription of Full-Length and Truncated mRNA Transcripts to Study Protein Translocation across the Endoplasmic Reticulum, Pages 19-35, REID GILMORE, PAULA COLLINS, JULIE JOHNSON, KENNAN KELLARIS, PETER RAPIEJKO
Probing the Molecular Environment of Translocating Polypeptide Chains by Cross-Linking, Pages 37-58, DIRK GÖRLICH, TEYMURAS V. KURZCHALIA, MARTIN WIEDMANN, TOM A. RAPOPORT
Reconstitution of Secretory Protein Translocation from Detergent-Solubilized Rough Microsomes, Pages 59-81, CHRISTOPHER NICCHITTA, GIOVANNI MIGLIACCIO, GÜNTER BLOBEL
Perforated Cells for Studying Intracellular Membrane Transport, Pages 85-108, MARK K. BENNETT, ANGELA WANDINGER-NESS, IVAN DE CURTIS, CLAUDE ANTONY, KAI SIMONS, JÜRGEN KARTENBECK
Delivery of Macromolecules into Cells Expressing a Viral Membrane Fusion Protein, Pages 109-130, HARMA ELLENS, STEPHEN DOXSEY, JEFFREY S. GLENN, JUDITH M. WHITE
Digitonin Permeabilization Procedures for the Study of Endosome Acidification and Function, Pages 131-149, RUBEN DIAZ, PHILIP D. STAHL
Reconstitution of Intracellular Vesicle Fusion in a Cell-Free System after Receptor-Mediated Endocytosis, Pages 153-170, LUIS S. MAYORGA, RUBEN DIAZ, PHILIP D. STAHL
Immunoisolation Using Magnetic Solid Supports: Subcellular Fractionation for Cell-Free Functional Studies, Pages 171-198, KATHRYN E. HOWELL, RUTH SCHMID, JOHN UGELSTAD, JEAN GRUENBERG
Endosome and Lysosome Purification by Free-Flow Electrophoresis, Pages 199-214, MARK MARSH
Immunoperoxidase Methods for the Localization of Antigens in Cultured Cells and Tissue Sections by Electron Microscopy, Pages 217-233, WILLIAM J. BROWN, MARILYN G. FARQUHAR
Postembedding Labeling on Lowicryl K4M Tissue Sections: Detection and Modification of Cellular Components, Pages 235-273, J. ROTH
Postembedding Detection of Acidic Compartments, Pages 275-284, RICHARD G.W. ANDERSON
Remodeling of Glycoprotein Oligosaccharides after Endocytosis: A Measure of Transport into Compartments of the Secretory Apparatus, Pages 287-298, MARTIN D. SNIDER
Enzymatic Approaches for Studying the Structure, Synthesis, and Processing of Glycoproteins, Pages 299-327, ANTHONY L. TARENTINO, ROBERT B. TRIMBLE, THOMAS H. PLUMMER JR
Separation and Analysis of Glycoprotein Oligosaccharides, Pages 329-371, RICHARD D. CUMMINGS, ROBERTA K. MERKLE, NANCY L. STULTS
Using Temperature-Sensitive Mutants of VSV to Study Membrane Protein Biogenesis, Pages 375-400, JOHN E. BERGMANN
Analysis of the Synthesis, Intracellular Sorting, and Function of Glycoproteins Using a Mammalian Cell Mutant with Reversible Glycosylation Defects, Pages 401-428, MONTY KRIEGER, PRANHITHA REDDY, KAREN KOZARSKY, DAVID KINGSLEY, LAWRENCE HOBBIE, MARSHA PENMAN
Replica Plating of Animal Cells, Pages 429-464, JEFFREY D. ESKO
The Use of Antiidiotype Antibodies for the Characterization of Protein–Protein Interactions, Pages 467-504, DAVID VAUX, STEPHEN D. FULLER
INDEX, Pages 505-507