Laboratory Methods in Enzymology: Protein Part B

Content: 
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xi-xii

Miscellaneous
Page xiii

Preface
Page xv
Jon Lorsch

Chapter One - In Vitro Synthesis of Proteins in Bacterial Extracts
Pages 3-15
Hani S. Zaher, Rachel Green

Chapter Two - Preparation of a Saccharomyces cerevisiae Cell-Free Extract for In Vitro Translation
Pages 17-28
Cheng Wu, Matthew S. Sachs

Chapter Three - Yeast Two-Hybrid Screen
Pages 31-51
Lauren Makuch

Chapter Four - UV Cross-Linking of Interacting RNA and Protein in Cultured Cells
Pages 53-66
Emi Sei, Nicholas K. Conrad

Chapter Five - Analysis of RNA–Protein Interactions by Cell Mixing
Pages 67-80
Sarah H. Stubbs, Nicholas K. Conrad

Chapter Six - General Protein–Protein Cross-Linking
Pages 81-87
Alice Alegria-Schaffer

Chapter Seven - Chromatin Immunoprecipitation and Multiplex Sequencing (ChIP-Seq) to Identify Global Transcription Factor Binding Sites in the Nematode Caenorhabditis Elegans
Pages 89-111
Cathleen M. Brdlik, Wei Niu, Michael Snyder

Chapter Eight - PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a Step-By-Step Protocol to the Transcriptome-Wide Identification of Binding Sites of RNA-Binding Proteins
Pages 113-161
Jessica Spitzer, Markus Hafner, Markus Landthaler, Manuel Ascano, Thalia Farazi, Greg Wardle, Jeff Nusbaum, Mohsen Khorshid, Lukas Burger, Mihaela Zavolan, Thomas Tuschl

Chapter Nine - Determining the RNA Specificity and Targets of RNA-Binding Proteins using a Three-Hybrid System
Pages 163-181
Yvonne Y. Koh, Marvin Wickens

Chapter Ten - Dissecting a Known RNA–Protein Interaction using a Yeast Three-Hybrid System
Pages 183-193
Yvonne Y. Koh, Marvin Wickens

Chapter Eleven - Identifying Proteins that Bind a Known RNA Sequence Using the Yeast Three-Hybrid System
Pages 195-214
Yvonne Y. Koh, Marvin Wickens

Author Index
Pages 215-217

Subject Index
Pages 219-223