Laboratory Methods in Cell Biology: Imaging

Content: 
Series Editors
Page ii

Front Matter
Page iii

Copyright
Page iv

Contributors
Pages ix-xii

Dedication
Page xiii

Preface: Laboratory Methods in Cell Biology
Page xv
P. Michael Conn

Chapter 1 - Structural Dynamics of Nucleic Acids by Single-Molecule FRET
Pages 1-37
Asger Christian Krüger, Lasse Lava Hildebrandt, Sofie Louise Kragh, Victoria Birkedal

Chapter 2 - Visualizing the Location and the Dynamics of Gene Expression in Living Animals through Bioluminescence Imaging
Pages 39-49
Xiaojuan Chen

Chapter 3 - Quality Control Protocol for Confocal Systems
Pages 51-58
Anda Cornea

Chapter 4 - Electron Microscopy of Bone
Pages 59-79
V. Everts, K.A. Hoeben

Chapter 5 - Immunostaining: Detection of Signaling Protein Location in Tissues, Cells and Subcellular Compartments
Pages 81-105
Biswanath Maity, David Sheff, Rory A. Fisher

Chapter 6 - Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein–Protein Interaction: How to Calculate Signal-to-Noise Ratio
Pages 107-121
Yutaka Kodama, Chang-Deng Hu

Chapter 7 - Immunohistochemical Detection of Neuropeptides/Transmitters in Mammalian Brain
Pages 123-147
Lothar Jennes

Chapter 8 - Intravital Imaging of Autoreactive T Cells in Living Animals
Pages 149-168
Naoto Kawakami, Ingo Bartholomäus, Marija Pesic, Nikolaos I. Kyratsous

Chapter 9 - Genetically Encoded Click Chemistry for Single-Molecule FRET of Proteins
Pages 169-187
Swati Tyagi, Edward A. Lemke

Chapter 10 - High-Throughput Methods for the Detection of Protein Overexpression Using Fluorescence Markers
Pages 189-208
Mads Gabrielsen, Andrew J. Roe, Karen McLuskey

Chapter 11 - Correlative Microscopy
Pages 209-255
Alexander A. Mironov, Galina V. Beznoussenko

Chapter 12 - Stereology of the Lung
Pages 257-294
Jan Philipp Schneider, Matthias Ochs

Chapter 13 - Quantitative 3D Cell-Based Assay Performed with Cellular Spheroids and Fluorescence Microscopy
Pages 295-309
Nariman Ansari, Stefanie Müller, Ernst H.K. Stelzer, Francesco Pampaloni

Chapter 14 - Optimizing Fluorescence Excitation and Detection for Intravital Two-Photon Microscopy
Pages 311-323
Dan Suan, Henry R. Hampton, Michio Tomura, Osami Kanagawa, Tatyana Chtanova, Tri Giang Phan

Chapter 15 - Two-Dimensional Crystallization by Dialysis for Structural Studies of Membrane Proteins by the Cryo-EM Method Electron Crystallography
Pages 325-337
Matthew C. Johnson, Ingeborg Schmidt-Krey

Chapter 16 - In situ Hybridization
Pages 339-359
Chung-Jui Tsai, Scott A. Harding

Chapter 17 - RNA Imaging: Tracking in Real-Time RNA Transport in Neurons Using Molecular Beacons and Confocal Microscopy
Pages 361-389
Angélica Zepeda, Clorinda Arias, Fabian Flores-Jasso, Luis Vaca

Index
Pages 391-404

Volumes in Series
Pages 405-414

Color Plate
Pages 1-16