Laboratory Methods in Cell Biology

Content: 
Series Editors
Page ii

Front Matter
Page iii

Copyright
Page iv

Contributors
Pages xi-xvi

Series Page
Page xvii

Preface: Laboratory Methods in Cell Biology
Page xix
P. Michael Conn

Chapter 1 - Extraction/Characterization of DNA
Pages 1-32
Leland J. Cseke, Joseph R. Herdy

Chapter 2 - Co-Immunoprecipitation: Isolation of Protein Signaling Complexes from Native Tissues
Pages 33-54
Adele Stewart, Rory A. Fisher

Chapter 3 - Real-Time PCR
Pages 55-68
Yibing Jia

Chapter 4 - Single-Cell Gel Electrophoresis Combined with Lesion-Specific Enzymes to Measure Oxidative Damage to DNA
Pages 69-92
Andrew R. Collins, Amaya Azqueta

Chapter 5 - High-Throughput Methods for the Identification of Protein Purification Conditions Using a Cleavable Tag System
Pages 93-110
Mads Gabrielsen, Katherine S.H. Beckham, Andrew J. Roe

Chapter 6 - Two-Dimensional Difference Gel Electrophoresis (2D DIGE)
Pages 111-141
Jonathan S. Minden

Chapter 7 - Optimization of Cell Cycle Measurement by Time-Lapse Microscopy
Pages 143-161
Gabor Nagy, Gabor Kiraly, Gaspar Banfalvi

Chapter 8 - Transfection Methods
Pages 163-182
A. Ruedel, A.K. Bosserhoff

Chapter 9 - Cell Enrichment from Human Blood through Red Cell Lysis
Pages 183-192
Brandon L. Miller, Jeffrey J. Chalmers, William G. Lowrie

Chapter 10 - Magnetic, Batch Separation
Pages 193-201
Jeffrey J. Chalmers,, William G. Lowrie

Chapter 11 - Lateral Flow Sandwich Assay Utilizing Upconverting Phosphor (UCP) Reporters
Pages 203-234
Elisa M. Tjon Kon Fat, William R. Abrams, R. Sam Niedbala, Paul L.A.M. Corstjens

Chapter 12 - In Vivo Modulation of MicroRNA Levels during Spinal Cord Regeneration
Pages 235-246
Juan Felipe Diaz Quiroz, Karen Echeverri

Chapter 13 - CometChip: Single-Cell Microarray for High-Throughput Detection of DNA Damage
Pages 247-268
Jing Ge, David K. Wood, David M. Weingeist, Sangeeta N. Bhatia, Bevin P. Engelward

Chapter 14 - Purification of Cardiac Cells
Pages 269-279
Fumiyuki Hattori, Keiichi Fukuda

Chapter 15 - Tube Formation In Vitro Angiogenesis Assay
Pages 281-293
Diego Guidolin, Giovanna Albertin

Chapter 16 - Processing of Transmission Electron Microscopy Images for Single-Particle Analysis of Macromolecular Complexes
Pages 295-310
Zaelle Devaux, Carlos Oscar S. Sorzano, Slavica Jonic

Chapter 17 - An Asymmetric 3D In Vitro Assay for the Study of Tumor Cell Invasion
Pages 311-328
Vera Brekhman, Gera Neufeld

Chapter 18 - Isolation of Myoblasts from Muscle Tissue by Preplating
Pages 329-337
Yoon Ghil Park, Ahmi Baek, Sunmi Kim

Chapter 19 - High-Content Small Molecule Screening Strategies for Stem-Cell-Derived Motor Neurons
Pages 339-359
Eunju Pack-Chung, Courtney A. Ackeifi, Lida Katsimpardi, Kevin J. Kim, Maureen Sherry Lynes, Nina R. Makhortova, Lee L. Rubin

Chapter 20 - A Simple Technique for Quantifying Apoptosis in 96-Well Plates
Pages 361-368
Shilo M. Smith, Deborah Ribble, Nathaniel B. Goldstein, David A. Norris, Yiqun G. Shellman

Chapter 21 - Cell Screening Using Resistive-Pulse Sensing
Pages 369-387
Karthik Balakrishnan, Lydia L. Sohn

Chapter 22 - Quantitative Analysis of Basic Motile Behavior in Amoeboid Cells
Pages 389-402
Deborah Wessels, Spencer Kuhl, David R. Soll

Chapter 23 - Single Cell Deposition
Pages 403-420
Jun Liu, Christopher Moraes, Zhe Lu, Craig Simmons, Yu Sun

Chapter 24 - Enzyme Sensors for Living Cells
Pages 421-437
Ning Chen, Shen Tang, Shenghui Xue, Jenny J. Yang

Chapter 25 - Myoblast Purifying by Magnetic-Activated Cell Sorting (MACS)
Pages 439-447
Yoon Ghil Park, Ahmi Baek, Sunmi Kim

Color Plates
Pages 1-10

Subject Index
Pages 449-464

Volumes in Series
Pages 465-474