Laboratory Exercises in Organismal and Molecular Microbiology

Cover......Page 1
Preface......Page 2
Safety Guidelines for the Microbiology Laboratory......Page 4
1. Structure, Function, and Use of the Microscope......Page 7
2. Micro. Comparisons of Microorganisms, Multi. Parasites & Micro. Invert.......Page 21
3. Microbial Procaryotes:Bacteria and Cyanobacteria......Page 29
4. Microbial Eucaryotes: Fungi......Page 36
5. Microbial Eucaryotes: Protozoans and Algae......Page 42
6. Flatworms and Roundworms......Page 50
7. Zooplankton......Page 58
8. Disease Vectors......Page 64
9. Negative Stain......Page 68
10. Smear Preparation and the Simple Stain......Page 72
11. Gram Stain......Page 78
12. Acid-fast Stain......Page 85
13. Spore Stain......Page 88
14. Capsule and Flagella Stains......Page 94
15. The Staining Characterization of a Bacterial Unknown......Page 100
16. Bacteria & Fungi in the Lab.Environ: The Necessity of Aseptic Technique......Page 107
17. Preparation and Inoculation of Growth Media......Page 116
18. Culture Characterization of Bacteria......Page 129
19. Biochemical Tests Used to Identify Bacteria......Page 136
20. Application: Identification of a Nonclinical Bacterial Unknown......Page 144
21. Isolation and Identification of Staphylococci from the Skin......Page 147
22. Isolation and Identification of Streptococci from the Throat......Page 154
23. Iden. of Ent. Bacteria, Enterotubes, a Rapid System to Iden. Ent. Bacteria......Page 160
24. Isolation and Identification of Bacteria from the Urinary Tract......Page 170
25. Assessing Antibiotic Effectiveness: The Kirby-Bauer Method......Page 176
26. Identification of a Clinical Bacterial Unknown......Page 184
27. Killing Bacteria with High Temperature......Page 190
28. Skin Disinfection: Evaluating Antiseptics and Hand Sanitizers......Page 194
29. Cleaning Countertops with Disinfectants......Page 200
30. Bacteriological Examination of Drinking Water Using the MPN Method......Page 203
31. Bacterial DNA Isolation and Southern Analysis......Page 210
32. Mutagenesis in Bacteria: The Ames Test......Page 225
33. Plasmid Isolation and Restriction Mapping......Page 231
34. Acquiring Antibiotic Resistance Through Bacterial Transformation......Page 239
35. Acquiring Antibiotic Resistance Through Bacterial Conjugation......Page 247
36. Viral DNA Fingerprinting......Page 252
37. Isolation of Bacteriophage from Sewage and Determination of Phage Titer......Page 261
38. The Virus Infection Cycle: The One-step Growth Curve......Page 269
39. Infection of Plant Leaves with Tobacco Mosaic Virus......Page 275
40. Identification and Enumeration of White Blood Cells......Page 281
41. Antigen-Antibody Precipitation Reactions and Determination of Antibody Titer......Page 288
42. Agglutination Reactions: ABO Blood Typing......Page 294
43. Immunodiffusion: Antigen-Antibody Precipitatioin Reactions in Gels......Page 300
44. Enzyme-linked Immunosorbent Assay (ELISA)......Page 309
45. Bacterial Protein Fingerprinting and Western Blotting......Page 315
46. The Neutralization of Viruses by Antibodies......Page 327
Credits......Page 334
Index......Page 335
Class Notes......Page 343