Laboratory Applications in Microbiology: A Case Study Approach

Title
Contents
Case Study Exercise 1 Safety Considerations in the Microbiology Laboratory
Case Study Exercise 2 Microscopy and Measurement of Microscopic Specimens
Case Study Exercise 3 Identification and Classification of Algae
Case Study Exercise 4 Survey of Medically Important Protozoa
Case Study Exercise 5 Identification and Classification of Fungi
Case Study Exercise 6 Ubiquity of Microorganisms
Case Study Exercise 7 Aseptic Techniques
Case Study Exercise 8 Pure Culture Techniques
Case Study Exercise 9 Simple Staining, Negative Staining, and Gram Staining
Case Study Exercise 10 Capsular Staining
Case Study Exercise 11 Acid-Fast and Endospore Staining
Case Study Exercise 12 Viable Plate Count
Case Study Exercise 13 Cultivation of Anaerobes
Case Study Exercise 14 Temperature Effects on Bacterial Growth and Survival
Case Study Exercise 15 pH and Microbial Growth
Case Study Exercise 16 Effects of Osmotic Pressure on Bacterial Growth
Case Study Exercise 17 Lethal Effects of Ultraviolet Light
Case Study Exercise 18 Evaluation of Disinfectants: Use-Dilution Method
Case Study Exercise 19 Effectiveness of Hand Scrubbing
Case Study Exercise 20 Antimicrobic Sensitivity Testing: Kirby-Bauer, Tube Dilution, and E-Test Methods
Case Study Exercise 21 Phage Typing of Bacteria
Case Study Exercise 22 Simulated Epidemic
Case Study Exercise 23 Morbidity and Mortality Weekly Report
Case Study Exercise 24 Bacterial Transformation
Case Study Exercise 25 The Ames Test
Case Study Exercise 26 DNA Extraction from Bacterial Cells
Case Study Exercise 27 DNA Profiling
Case Study Exercise 28 Measures of Water Quality: Most Probable Number Procedure
Case Study Exercise 29 Measures of Water Quality: Membrane Filtration Method
Case Study Exercise 30 Measures of Milk Quality: Methylene Blue Reductase Test
Case Study Exercise 31 Bacterial Counts of Food
Case Study Exercise 32 Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacteriaceae
Case Study Exercise 33 Isolation and Identification of Staphylococci
Case Study Exercise 34 Isolation and Identification of Streptococci
Case Study Exercise 35 Blood Typing
Case Study Exercise 36 Differential White Blood Cell Count
Case Study Exercise 37 Slide Agglutination
Case Study Exercise 38 Enzyme-Linked Immunosorbent Assay (ELISA)
Case Study Exercise 39 Identification of Bacterial Unknowns
Exercise 40 Colony Morphology
Exercise 41 Growth in Solid and Liquid Media
Exercise 42 Simple Stain
Exercise 43 Negative Stain
Exercise 44 Capsule Stain
Exercise 45 Gram Stain
Exercise 46 Endospore Stain
Exercise 47 Acid-Fast Stain
Exercise 48 Motility Methods: Wet Mount and Hanging Drop
Exercise 49 Flagella Stain
Exercise 50 Streak-Plate Isolation
Exercise 51 Loop Dilution
Exercise 52 Spread-Plate
Exercise 53 Fluid Thioglycollate Medium
Exercise 54 Mannitol Salt Agar
Exercise 55 MacConkey Agar
Exercise 56 Desoxycholate Agar
Exercise 57 Endo Agar
Exercise 58 Eosin Methylene Blue Agar
Exercise 59 Hektoen Enteric Agar
Exercise 60 Xylose Lysine Desoxycholate Agar
Exercise 61 Blood Agar
Exercise 62 Motility Media
Exercise 63 SIM Medium
Exercise 64 Kligler’s Iron Agar
Exercise 65 Triple Sugar Iron Agar
Exercise 66 Lysine Iron Agar
Exercise 67 Litmus Milk
Exercise 68 Oxidation-Fermentation Test
Exercise 69 Phenol Red Broth
Exercise 70 Purple Broth
Exercise 71 Methyl Red and Voges-Proskauer Tests
Exercise 72 Catalase Test
Exercise 73 Oxidase Test
Exercise 74 Nitrate Reduction Test
Exercise 75 Coagulase Test
Exercise 76 Citrate Test
Exercise 77 Malonate Test
Exercise 78 Decarboxylation Test
Exercise 79 Phenylalanine Deaminase Test
Exercise 80 Bile Esculin Test
Exercise 81 Starch Hydrolysis
Exercise 82 ONPG Test
Exercise 83 Urease Test
Exercise 84 Casease Test
Exercise 85 Gelatinase Test
Exercise 86 DNase Test
Exercise 87 Lipase Test
Exercise 88 CAMP Test
Exercise 89 PYR Test
Exercise 90 API 20E System
Exercise 91 Enterotube II System
Exercise 92 Antibiotic Disk Sensitivity Tests
Exercise 93 ?-Lactamase Test
Exercise 94 Viable Plate Count
Exercise 95 Direct Cell Count
Appendix A: Spectrophotometric Determination of Bacterial Growth: Use of the Spectrophotometer
Appendix B : Use of Pipettes in the Laboratory
Appendix C : Preparation of Culture Media
Appendix D : Media, Reagents, and Stain Formulas
Appendix E : Data Sheet for Unknown Identification
Glossary
Credits
Index