Investigation of Membrane-Located Receptors

Front Matter....Pages i-xvi
Front Matter....Pages 1-1
Where Do We Stand on Receptors Today?....Pages 3-6
Strategy in Receptor Binding Studies....Pages 7-15
Principles of Receptor Binding Assays: The Gaba Receptor....Pages 17-29
Specificity Criteria for Receptor Sites in Binding Studies....Pages 31-46
Target Size Analysis of Cell Surface Receptors and Adenylate Cyclase....Pages 47-58
Determination of Molecular Weights of Receptors in Solution and in Situ ....Pages 59-82
Development and Purification of Calmodulin Andibodies: Use in Elucidating Multiple Functions of the Protein in Eukaryotic Cells....Pages 83-93
Approaches to Isolating Minor Membrane Components Using Monoclonal Antibodies, as in Purifying 5′-Nucleotidase....Pages 95-106
Receptor Purification: A Brief Overview....Pages 107-110
Isolation of Purified Membranes and Membranous Cell Components for Receptor Studies....Pages 111-118
Front Matter....Pages 119-119
Molecular Genetics Applied to Receptor Analysis....Pages 121-132
Front Matter....Pages 133-133
Isolation and Characterization of Spiny- (Clathrin)-Coated Membranes and Vesicles from Rodent Liver and Role in Receptor-Mediated Porcesses....Pages 135-141
Hormone Receptor — Adenylate Cyclase Interaction: Superpotency, Superaffinity and Desensitization Phenomena....Pages 143-150
Analyzing the Steps in Coupling the Opiate Receptor to Adenylate Cyclase in Neuroblastoma × Glioma Hybrid Cells Ng108-15....Pages 151-161
Architecture of Plasma Membrane Hormone Receptor-Effector Systems....Pages 163-180
Epithelial Sodium Channels: Ligand Binding Techniques in Intact Tissues and Homogenates....Pages 181-190
Role of Internal Membrane-Located Receptors in Intracellular Trafficking....Pages 191-201
Subcellular Strategies for Tracing Receptor-Mediated Ligand Uptake Pathways in Liver....Pages 203-210
Receptor-Mediated Transfer of Proteins from Blood to Bile....Pages 211-224
Involvement of Microtubules in Insulin-Receptor Turnover in Cardiac Myocytes....Pages 225-230
Front Matter....Pages 231-231
Thyroid Membrane Nad-Glycohydrolase and Adp•Ribosyl-Transferase, Possibly Relevant to TSH Action....Pages 233-236
Isolated Adenylate Cyclase and β-Adrenergic Receptor....Pages 237-238
Insulin Internalization as Studied by Subcellular Fractionation and Electron-Microscopic Radioautography....Pages 239-242
Synaptosomes and Synaptic Membranes: Isolation and Morphology....Pages 243-248
Front Matter....Pages 249-249
Characterization of Cell-Surface Receptors for Calcitonin in Hormone-Responsive Cancer Cells....Pages 251-262
Binding Sites for Pituitary Growth Hormone and Prolactin in the Liver and Mammary Gland of the Pregnant Rabbit....Pages 263-275
Affinity Labelling of the Thyrotropin Receptor....Pages 277-293
Affinity Labelling Techniques for Low-Abundance Membrane Components: Peptide Hormone Receptors and the D-Glucose Transporter....Pages 295-302
A Glucose-Sensitive Glycoprotein Recognition System on Rat Hepatic Endothelial Cells....Pages 303-309
Plasma Lipoproteins and the Regulation of Cellular Function....Pages 311-316
Liposomes Targeted to Cellular Receptors....Pages 317-330
Targeted Drug Carriers: Biological Activity of the N -Acetylcysteine-Liposome System....Pages 331-336
Front Matter....Pages 337-337
Hepatic Growth Hormone/Prolactin Receptors in Male Rats: Intracellular Localization, Induction And Internalization....Pages 339-342
The Ldl Receptor: Functional Studies And Partial Characterization....Pages 343-344
The Receptor for Immunoglobulin E on Mast Cells and Basophils....Pages 345-350
Preparation of Immunoliposomes....Pages 351-355
Targeting Toxins by Conjugation to Antibodies: The Effect of the Linkage Group on Biological Activity....Pages 357-358
Antibody-Targeted Liposomes: Specific Cell Binding and Cytotoxicity....Pages 359-359
Liposomes as Vehicles for Inhibitors of Intracytoplasmic Protein Synthesis....Pages 361-366
Front Matter....Pages 367-367
Examination of Ligand Occupation and the Permeability Response of the Nicotinic Acetylcholine Receptor on Intact Cells....Pages 369-380
Front Matter....Pages 367-367
Group-Specific Reagents as Probes for Measuring Agonist-Mediated Conformational Changes of β-Adrenergic and Muscarinic Acetylcholine Receptors....Pages 381-388
Physico-Chemical Studies of β-Adrenergic Receptors on Intact Cells....Pages 389-395
 In Vitro Regulation of β-Adrenergic Receptors....Pages 397-404
Characterization and Isolation of Brain Dopamine (D 2 ) Receptors....Pages 405-416
Front Matter....Pages 417-417
Affinity Labelling of a Cholinergic Nicotinic Receptor in Cultured Cells....Pages 419-420
Solubilization and Characterization of Muscarinic Acetylcholine Receptors....Pages 421-423
Antibodies to a Covalent Antagonist used to Isolate the Muscarinic Cholinergic Receptor from Rat Brain....Pages 425-427
Myasthenia Gravis — Some Experimental Approaches....Pages 429-431
Guanine Nucleotide Regulation of Agonist Binding to Hepatic α 1 -Adrenergic Receptors....Pages 433-434
Biochemical and Immunological Methods to Purify the β-Adrenergic Receptors....Pages 435-441
Front Matter....Pages 443-443
Solubilization and Characterization of Active Opiate Binding Sites....Pages 445-453
Selective Tolerance Development Facilitates a Differentiation of Multiple Opiate Receptors....Pages 455-460
Subcellular Fractionation of Insect Muscle....Pages 461-468
The Receptor for α-Latrotoxin, Studied in Pre-Synaptic Membranes and Membrane Extracts....Pages 469-479
Allosteric Interactions in Receptor Binding Studies....Pages 481-486
Front Matter....Pages 487-487
A Slowly Dissociating form of Bovine Hippocampal Synaptic Membrane Opiate Receptors....Pages 489-492
Binding of Reversible and Irreversible Ligands to Rat Brain Opiate Receptors....Pages 493-496
Affinity Probes for Opioid Receptors in Synaptosomes....Pages 497-498
Biochemical Pharmacology of Substance P Receptors....Pages 499-501
Substance P-Stimulated Phosphatidylinositol Breakdown in Guinea Pig Ileum....Pages 503-504
Front Matter....Pages 487-487
Soluble GABA/Benzodiazepine/Barbiturate Receptor Interactions in Mammalian Brain....Pages 505-507
Characterization and Purification of the Glycine Receptor of Rat Spinal Cord....Pages 509-511
Observations on Receptor Sites for Dihydropyridines....Pages 513-518
Specific High-Affinity Membrane Receptors for Biologically Active Phorbol and Ingenol Esters....Pages 519-521
Molecular Graphics and Computational Chemistry Techniques for Drug Design....Pages 523-530
Computer-Aided Conformational Approaches to Modelling....Pages 537-542
An Editor’s Guide to Receptor Studies with Cell-Free Systems....Pages 543-544
Back Matter....Pages 545-558