Intracellular Lipid Transport: Methods and Protocols

Front Matter ....Pages i-xi
 Analysis of Phosphatidylinositol Transfer at ER-PM Junctions in Receptor-Stimulated Live Cells  (Chi-Lun Chang, Jen Liou)....Pages 1-11
 Monitoring Non-vesicular Transport of Phosphatidylserine and Phosphatidylinositol 4-Phosphate in Intact Cells by BRET Analysis  (Mira Sohn, Daniel J. Toth, Tamas Balla)....Pages 13-22
 Development of Nonspecific BRET-Based Biosensors to Monitor Plasma Membrane Inositol Lipids in Living Cells  (József T. Tóth, Gergő Gulyás, László Hunyady, Péter Várnai)....Pages 23-34
 Following Anterograde Transport of Phosphatidylserine in Yeast in Real Time  (Juan Martín D’Ambrosio, Véronique Albanèse, Alenka Čopič)....Pages 35-46
 Imaging Lipid Metabolism at the Golgi Complex  (Serena Capasso, Giovanni D’Angelo)....Pages 47-56
 Advanced In Vitro Assay System to Measure Phosphatidylserine and Phosphatidylethanolamine Transport at ER/Mitochondria Interface  (Yasushi Tamura, Rieko Kojima, Toshiya Endo)....Pages 57-67
 Measurement of Lipid Transport in Mitochondria by the MTL Complex  (Juliette Jouhet, Valérie Gros, Morgane Michaud)....Pages 69-93
 Bi- and Trifunctional Lipids for Visualization of Sphingolipid Dynamics within the Cell  (Doris Höglinger)....Pages 95-103
 Indirect Lipid Transfer Protein Activity Measurements Using Quantification of Glycosphingolipid Production  (Anders P. E. Backman, Josefin Halin, Matti A. Kjellberg, Peter Mattjus)....Pages 105-114
 Measurement of Intracellular Sterol Transport in Yeast  (Neha Chauhan, Julian A. Jentsch, Anant K. Menon)....Pages 115-136
 Intracellular and Plasma Membrane Cholesterol Labeling and Quantification Using Filipin and GFP-D4  (Léa P. Wilhelm, Laetitia Voilquin, Toshihide Kobayashi, Catherine Tomasetto, Fabien Alpy)....Pages 137-152
 Monitoring and Modulating Intracellular Cholesterol Trafficking Using ALOD4, a Cholesterol-Binding Protein  (Shreya Endapally, Rodney E. Infante, Arun Radhakrishnan)....Pages 153-163
 Measurement of Lysophospholipid Transport Across the Membrane Using Escherichia coli Spheroplasts  (Yibin Lin, Lei Zheng, Mikhail Bogdanov)....Pages 165-180
 Preparation of Proteoliposomes with Purified TMEM16 Protein for Accurate Measures of Lipid Scramblase Activity  (Janine Denise Brunner, Stephan Schenck)....Pages 181-199
 In Vitro Assays to Measure the Membrane Tethering and Lipid Transport Activities of the Extended Synaptotagmins  (Xin Bian, Pietro De Camilli)....Pages 201-212
 Determining the Lipid-Binding Specificity of SMP Domains: An ERMES Subunit as a Case Study  (Andrew P. AhYoung, Pascal F. Egea)....Pages 213-235
 In Vitro Measurement of Sphingolipid Intermembrane Transport Illustrated by GLTP Superfamily Members  (Roopa Kenoth, Rhoderick E. Brown, Ravi Kanth Kamlekar)....Pages 237-256
 Purification and Characterization of Human Niemann–Pick C1 Protein  (Xin Gong, Hongwu Qian)....Pages 257-267
 In Vitro Strategy to Measure Sterol/Phosphatidylinositol-4-Phosphate Exchange Between Membranes  (Nicolas-Frédéric Lipp, Guillaume Drin)....Pages 269-292
 Determination of Ligand Binding Affinity and Specificity of Purified START Domains by Thermal Shift Assays Using Circular Dichroism  (Danny Létourneau, Jean-Guy LeHoux, Pierre Lavigne)....Pages 293-306
 Synthesis of Fluorescent Membrane-Spanning Lipids for Studies of Lipid Transfer and Membrane Fusion  (Günter Schwarzmann)....Pages 307-324
 Setting Up All-Atom Molecular Dynamics Simulations to Study the Interactions of Peripheral Membrane Proteins with Model Lipid Bilayers  (Viviana Monje-Galvan, Linnea Warburton, Jeffery B. Klauda)....Pages 325-339
Back Matter ....Pages 341-345