Interacting Protein Domains: Their Role in Signal and Energy Transduction

Front Matter....Pages I-X
Front Matter....Pages 1-1
Control of Cellular Processes by Reversible Protein Phosphorylation....Pages 3-8
From Phosphorylase to Phosphorylase Kinase....Pages 9-14
Protein Kinase X: A Novel Human Protein Kinase closely related to the Catalytic Subunit of cAMP-dependent Protein Kinase....Pages 15-18
Interaction of Protein Kinase A c from Ascaris suum with Proteins and Peptides: Comparison with the Mammalian Enzyme....Pages 19-24
Front Matter....Pages 25-25
Interaction Studies Using Biosensors....Pages 27-36
Advances in Determination of Protein Structure by X-ray Diffraction Methods....Pages 37-44
Spectroscopical studies on the interacti on between ww domain and proline-rich peptides ....Pages 45-48
Novel Microscope-Based Approaches for the Investigation of Protein-Protein Interactions in Signal Transduction....Pages 49-52
Binding studies with SH2 domains from the phosphotyrosine kinase ZAP70 using surface plasmon resonance and scintillation proximity assays....Pages 53-57
Leucine Zipper mediated Homodimerization of Autoantigen L7 analyzed by Electrospray Ionization Mass Spectrometry and Yeast Two Hybrid Interactions....Pages 59-62
Proteins at Work: Time-Resolved FTIR Studies of Bacteriorhodopsin and the GTP-Binding Protein P21....Pages 63-72
Front Matter....Pages 73-73
Downstream Signaling from Phosphoinositide 3-Kinase....Pages 75-81
Phosphoinositide-3-Kinase Mediated Activation of JNK by the ßPDGFR....Pages 83-86
Regulation of Phospholipase C isozymes....Pages 87-92
Substrate Binding and Catalytic Mechanism in Phospholipase C from Bacillus cereus ....Pages 93-96
Identification of Three Active Site Residues Involved in Substrate Binding by Human 43 kDa-D- myo -Inositol 1,4,5- Tris phosphate 5-Phosphatase....Pages 97-100
Structural basis of protein-ligand interactions in the Pleckstrin Homology domain....Pages 101-104
Front Matter....Pages 105-105
Cell Signaling by Tyrosine Phosphorylation: The Other Side of the Coin....Pages 107-112
Binding studies on the neuronal isoform of the non-receptor protein tyrosine kinase pp60 c-src , pp60 c-srcN and potential target proteins....Pages 113-116
The switch cycle of the Ras protein and its role in signal transduction....Pages 117-138
Front Matter....Pages 105-105
 In vivo quantitative assessment of Ras/Raf interaction using the two-hybrid system....Pages 139-142
 in vivo Analysis of C-Raf1 — 14-3-3 Interaction....Pages 143-146
Signal Transduction through the MAP kinase Pathway....Pages 147-151
Identification and Characterization of MKKX, a novel mammalian MAP kinase kinase....Pages 153-156
Interleukin-1 activates a novel p54 MAP Kinase Kinase in rabbit liver....Pages 157-160
The Protein Kinase B Family — Structure, Regulation and Function....Pages 161-170
Regulation of p70 s6k ....Pages 171-185
Rapamycin, FRAP and the Control of 5’TOP mRNA Translation....Pages 187-194
Structure, Regulation and Targeting of Protein Phosphatase 2A....Pages 195-206
The Constitutive Activation of MET, RON and SEA Genes Induces Different Biological Responses....Pages 207-212
Nucleoside Diphosphate Kinase: Effect of the P100S Mutation on Activity and Quaternary Structure....Pages 213-216
Front Matter....Pages 217-217
Interaction of adenylyl cyclase type 1 with βγ subunits of heterotrimeric G-proteins....Pages 219-222
Regulation of G-protein Activation in Retinal Rods by Phosducin....Pages 223-226
Selective interactions of the rat μ-opioid receptor and a chimeric μ-opioid receptor expressed in COS-7 cells with multiple G proteins....Pages 227-230
Structural requirements for the EF-Tu-directed kinase....Pages 231-234
Effect of the Phytotoxin Fusicoccin on Plant Plasma Membrane H + -ATPase Expressed in Yeast....Pages 235-238
Organization and putative regulation of the glucose-specific phosphotransferase system from Staphylococcus carnosus ....Pages 239-244
Front Matter....Pages 245-245
Single Molecule Myosin Mechanics Measured Using Optical Trapping....Pages 247-259
Studies on the ATP-binding Site of Actin Using Site-directed Mutagenesis....Pages 261-264
Characterisation of the actin-binding protein Insertin....Pages 265-268
Front Matter....Pages 245-245
Cardiac Troponin....Pages 269-279
Studies on the function of the different phosphoforms of cardiac troponin I....Pages 281-284
Back Matter....Pages 287-292