Instant Noties in Molecular Biology

BOOK COVER......Page 1
TITLE......Page 4
COPYRIGHT......Page 5
CONTENTS......Page 6
ABBREVIATIONS......Page 10
PREFACE TO THE THIRD EDITION......Page 12
PREFACE TO THE SECOND EDITION......Page 13
PREFACE TO THE FIRST EDITION......Page 14
A1 CELLULAR CLASSIFICATION......Page 16
A2 SUBCELLULAR ORGANELLES......Page 19
A3 MACROMOLECULES......Page 22
A4 LARGE MACROMOLECULAR ASSEMBLIES......Page 26
B1 AMINO ACIDS......Page 30
B2 PROTEIN STRUCTURE AND FUNCTION......Page 33
B3 PROTEIN ANALYSIS......Page 40
C1 NUCLEIC ACID STRUCTURE......Page 48
C2 CHEMICAL AND PHYSICAL PROPERTIES OF NUCLEIC ACIDS......Page 55
C3 SPECTROSCOPIC AND THERMAL PROPERTIES OF NUCLEIC ACIDS......Page 59
C4 DNA SUPERCOILING......Page 62
D1 PROKARYOTIC CHROMOSOME STRUCTURE......Page 66
D2 CHROMATIN STRUCTURE......Page 68
D3 EUKARYOTIC CHROMOSOME STRUCTURE......Page 73
D4 GENOME COMPLEXITY......Page 78
D5 THE FLOW OF GENETIC INFORMATION......Page 83
E1 DNA REPLICATION: AN OVERVIEW......Page 88
E2 BACTERIAL DNA REPLICATION......Page 93
E3 THE CELL CYCLE......Page 97
E4 EUKARYOTIC DNA REPLICATION......Page 101
F1 MUTAGENESIS......Page 106
F2 DNA DAMAGE......Page 110
F3 DNA REPAIR......Page 113
F4 RECOMBINATION......Page 116
G1 DNA CLONING: AN OVERVIEW......Page 120
G2 PREPARATION OF PLASMID DNA......Page 125
G3 RESTRICTION ENZYMES AND ELECTROPHORESIS......Page 128
G4 LIGATION, TRANSFORMATION AND ANALYSIS OF RECOMBINANTS......Page 133
H1 DESIGN OF PLASMID VECTORS......Page 140
H2 BACTERIOPHAGE VECTORS......Page 144
H3 COSMIDS, YACS AND BACS......Page 149
H4 EUKARYOTIC VECTORS......Page 154
I1 GENOMIC LIBRARIES......Page 160
I2 CDNA LIBRARIES......Page 163
I3 SCREENING PROCEDURES......Page 168
J1 CHARACTERIZATION OF CLONES......Page 172
J2 NUCLEIC ACID SEQUENCING......Page 177
J3 POLYMERASE CHAIN REACTION......Page 182
J4 ORGANIZATION OF CLONED GENES......Page 187
J5 MUTAGENESIS OF CLONED GENES......Page 191
J6 APPLICATIONS OF CLONING......Page 195
K1 BASIC PRINCIPLES OF TRANSCRIPTION......Page 200
K2 ESCHERICHIA COLI RNA POLYMERASE......Page 203
K3 THE E. COLI SIGMA 70 PROMOTER......Page 205
K4 TRANSCRIPTION, INITIATION, ELONGATION AND TERMINATION......Page 208
L1 THE LAC OPERON......Page 214
L2 THE TRP OPERON......Page 218
L3 TRANSCRIPTIONAL REGULATION BY ALTERNATIVE SIGMA FACTORS......Page 222
M1 THE THREE RNA POLYMERASES: CHARACTERIZATION AND FUNCTION......Page 226
M2 RNA POL I GENES: THE RIBOSOMAL REPEAT......Page 228
M3 RNA POL III GENES: 5S AND tRNA TRANSCRIPTION......Page 232
M4 RNA POL II GENES: PROMOTERS AND ENHANCERS......Page 236
M5 GENERAL TRANSCRIPTION FACTORS AND RNA POL II INITIATION......Page 238
N1 EUKARYOTIC TRANSCRIPTION FACTORS......Page 242
N2 EXAMPLES OF TRANSCRIPTIONAL REGULATION......Page 248
O1 rRNA PROCESSING AND RIBOSOMES......Page 254
O2 tRNA PROCESSING AND OTHER SMALL RNAS......Page 260
O3 mRNA PROCESSING, hnRNPS AND snRNPS......Page 264
O4 ALTERNATIVE mRNA PROCESSING......Page 270
P1 THE GENETIC CODE......Page 274
P2 tRNA STRUCTURE AND FUNCTION......Page 278
Q1 ASPECTS OF PROTEIN SYNTHESIS......Page 284
Q2 MECHANISM OF PROTEIN SYNTHESIS......Page 288
Q3 INITIATION IN EUKARYOTES......Page 294
Q4 TRANSLATIONAL CONTROL AND POST-TRANSLATIONAL EVENTS......Page 298
R1 INTRODUCTION TO VIRUSES......Page 304
R2 BACTERIOPHAGES......Page 308
R3 DNA VIRUSES......Page 313
R4 RNA VIRUSES......Page 317
S1 ONCOGENES FOUND IN TUMOR VIRUSES......Page 322
S2 CATEGORIES OF ONCOGENES......Page 326
S3 TUMOR SUPPRESSOR GENES......Page 329
S4 APOPTOSIS......Page 333
T1 INTRODUCTION TO THE ’OMICS......Page 338
T2 GLOBAL GENE EXPRESSION ANALYSIS......Page 342
T3 PROTEOMICS......Page 347
T4 CELL AND MOLECULAR IMAGING......Page 352
T5 TRANSGENICS AND STEM CELL TECHNOLOGY......Page 357
U1 INTRODUCTION TO BIOINFORMATICS......Page 362
FURTHER READING......Page 372
INDEX......Page 377