Infrared analysis of peptides and proteins: principles and applications

Title Page......Page 1
Copyright......Page 2
Advisory Board......Page 3
Foreword......Page 4
Preface......Page 5
Acknowledgments......Page 7
1 Basic Aspects of the Technique and Applications of IR Spectroscopy of Peptides and Proteins......Page 8
IR Spectroscopy of Proteins and Peptides......Page 9
Protein Secondary Structure by Amide I vs. Amide III......Page 22
Modern advanced IR techniques......Page 38
Concluding Remarks......Page 40
References......Page 41
2 Interpreting IR Spectra of Peptides and Proteins......Page 44
Characteristics of Peptide Group Modes......Page 45
Future Directions......Page 52
Literature Cited......Page 57
3 FT-IR Spectroscopic Studies of Peptides: Potentials and Pitfalls......Page 60
Measurement and Analysis of Peptide FTIR spectra......Page 61
Information on Peptide Secondary Structure from FTIR Spectra......Page 65
The Problem of Peptide Aggregation......Page 77
Side Chains and Chromophores - Structure and Overlap with Amide Bands......Page 85
Hydrogen-Deuterium Exchange Analysis Using FTIR Spectroscopy......Page 89
Influence of Environment on Protein Infrared Spectra......Page 91
Complexity of Membrane Protein FTIR Spectra......Page 95
Future prospects......Page 97
References......Page 98
4 ATR-IR\rSpectroscopy: Orientation and Tertiary Structural Changes of Proteins or Peptides Inserted into a Lipid Bilayer......Page 102
Polarized ATR-FTIR for orientation determination in proteins and lipids - General principles......Page 103
Potentialities for orientation determination of membrane components - Alignment of the Apolipophorin-III α-helices in complexes with dimyristoyl-phosphatidylcholine......Page 106
Amide hydrogen/deuterium exchange kinetics......Page 110
References......Page 120
5 Determination of the Secondary Structure of Proteins from Amide I and Amide III IR Bands Using PLS Method......Page 123
Partial least-square method (PLS)......Page 125
Prediction of secondary structure contents......Page 127
Advantages and Disadvantages of the approach......Page 131
References......Page 134
6 Determination of Secondary Structure in Protein Aggregates Using ATR-FTIR......Page 136
Protein Aggregation......Page 137
Attenuated Total Reflectance (ATR) FTIR......Page 138
Partially-folded Intermediates As Aggregation Precursors......Page 140
ATR-FTIR Investigations Of Inclusion Bodies And Folding Aggregates......Page 141
ATR-FTIR Investigations Of Amyloid Fibrils......Page 143
Models For The Mechanism Of Protein Aggregation......Page 145
Concluding remarks......Page 146
Literature cited......Page 147
7 Thermal Denaturation of Elastase in the Presence and Absence of Guanidinium Chloride: An IR Spectroscopic and DSC Investigation......Page 149
Materials and Methods......Page 151
Results......Page 153
Discussion......Page 156
Conclusion......Page 160
Literature Cited......Page 161
INTRODUCTION......Page 163
The light-induced BR to Κ transition......Page 166
The thermal reactions; Κ →L→M→N→O→BR......Page 167
Acknowledgement:......Page 168
References......Page 170
9 Investigation of Cytokine−Receptor Interactions by Isotope-Edited FTIR Spectroscopy......Page 171
Isotope-Edited FTIR Spectroscopy......Page 172
References......Page 185
Author Index......Page 187
A......Page 188
F......Page 189
I......Page 190
O......Page 191
S......Page 192
X......Page 193