In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs

Front Matter....Pages i-xi
Front Matter....Pages 1-1
Introduction....Pages 3-5
Biomedical Significance and Development of “IVCT”....Pages 7-10
How to Perform “IVCT”....Pages 11-14
Technical Merits with “IVCT”....Pages 15-16
Front Matter....Pages 17-17
Histochemical Analyses of Living Mouse Liver Under Different Hemodynamic Conditions....Pages 19-23
Three-Dimensional Reconstruction of Liver Tissues with Confocal Laser Scanning Microscopy....Pages 25-28
Application of “In Vivo Cryotechnique” to Immunohistochemical Detection of Hypoxia in Mouse Liver Tissues Treated with Pimonidazole....Pages 29-32
Immunohistochemical Detection of Soluble Immunoglobulins in Small Intestines....Pages 33-38
Detection of MAPK Signal Transduction Proteins in an Ischemia/Reperfusion Model of Small Intestines....Pages 39-44
Histological Study and LYVE-1 Immunolocalization of Mesenteric Lymph Nodes....Pages 45-49
Distribution of Immunoglobulin-Producing Cells in Immunized Mouse Spleens....Pages 51-56
Alteration of Erythrocyte Shapes in Various Organs of Living Mice or Under Blood Flow Conditions....Pages 57-61
Dynamic Ultrastructure of Smooth Muscle Cells in Dystrophin-Deficient mdx or Normal scn Mice....Pages 63-66
Dynamic Bioimaging of Serum Proteins in Beating Mouse Hearts....Pages 67-76
Histochemical Analyses and Quantum Dot Imaging of Microvascular Blood Flow with Pulmonary Edema....Pages 77-81
Dynamic Ultrastructure of Pulmonary Alveoli of Living Mice Under Respiratory Conditions....Pages 83-86
Immunohistochemical Analysis of Various Serum Proteins in Living Mouse Thymus....Pages 87-92
Immunohistochemistry and Enzyme Histochemistry of HRP for Demonstration of Blood Vessel Permeability in Mouse Thymic Tissues....Pages 93-99
Immunolocalization of Serum Proteins in Living Mouse Glomeruli Under Various Hemodynamic Conditions....Pages 101-104
Immunohistochemical Analyses on Albumin and IgG in Acute Hypertensive Mouse Kidneys....Pages 105-108
Front Matter....Pages 17-17
Application of Novel “In Vivo Cryotechnique” in Living Animal Kidneys....Pages 109-113
Application of Periodic Acid-Schiff Fluorescence Emission for Immunohistochemistry of Living Mouse Renal Glomeruli....Pages 115-119
Immunohistochemical Analyses on Serum Proteins in Nephrons of Protein-Overload Mice....Pages 121-125
Immunohistochemical Analyses of Serum Proteins in Puromycin Aminonucleoside Nephropathy of Living Rat Kidneys....Pages 127-133
Immunolocalization of Phospho-Arg-Directed Protein Kinase-Substrate in Hypoxic Kidneys....Pages 135-140
Dynamic Ultrastructures of Renal Glomeruli in Living Mice Under Various Hemodynamic Conditions....Pages 141-143
Function of Dynamin-2 in the Formation of Discoid Vesicles in Urinary Bladder Umbrella Cells....Pages 145-147
Involvement of Follicular Basement Membrane and Vascular Endothelium in Blood-Follicle Barrier Formation of Mice....Pages 149-153
Permselectivity of Blood-Follicle Barriers in Mouse Polycystic Ovary Model....Pages 155-160
Application of “In Vivo Cryotechnique” to Immunohistochemical Study of Serum Albumin in Normal and Cadmium-Treated Mouse Testis Organs....Pages 161-163
Immunohistochemical Detection of Angiotensin II Receptors in Mouse Cerebellum....Pages 165-170
Application of “In Vivo Cryotechnique” to Immunohistochemical Analyses for Effects of Anoxia on Serum Immunoglobulin and Albumin Leakage Through Blood–Brain Barrier in Mouse Cerebellum....Pages 171-173
Extracellular Space in Central Nervous System....Pages 175-178
Overview on Recent Applications of In Vivo Cryotechnique in Neurosciences....Pages 179-183
Application of “In Vivo Cryotechnique” to Immunohistochemical Detection of Phosphorylated Rhodopsin in Light-Exposed Retina of Living Mouse....Pages 185-187
Application of “In Vivo Cryotechnique” to Immunohistochemical Detection of Glutamate in Mouse Retina Inner Segment of Photoreceptors....Pages 189-191
Application of “In Vivo Cryotechnique” to Immunohistochemical Study of Mouse Sciatic Nerves Under Various Stretching Conditions....Pages 193-196
Front Matter....Pages 197-197
Freeze-Substitution (FS) Fixation Method....Pages 199-202
Front Matter....Pages 203-203
Cryotechniques and Freeze-Substitution for Immunostaining of Intranuclear Antigens and Fluorescence In Situ Hybridization....Pages 205-209
Replica Immunoelectron Microscopy for Caveolin in Living Smooth Muscle Cells....Pages 211-214
Front Matter....Pages 203-203
Application of “In Vivo Cryotechnique” to Detection of Injected Fluorescence-Conjugated IgG in Living Mouse Organs....Pages 215-217
Application of “In Vivo Cryotechnique” to Visualization of Microvascular Blood Flow in Mouse Kidney by Quantum Dot Injection....Pages 219-221
Front Matter....Pages 223-223
Application of “In Vivo Cryotechnique” to Raman Microscopy of Freeze-Dried Mouse Eyeball Slice....Pages 225-227
Application of “In Vivo Cryotechnique” to Detection of Erythrocyte Oxygen Saturation in Mouse Blood Vessels with Confocal Raman Cryomicroscopy....Pages 229-231
Front Matter....Pages 233-233
Imaging of Thrombosis and Microcirculation in Mouse Lungs of Initial Melanoma Metastasis....Pages 235-239
Differential Distribution of Blood-Derived Proteins in Xenografted Human Adenocarcinoma Tissues....Pages 241-245
Front Matter....Pages 247-247
Morphological and Histochemical Analysis of Living Mouse Livers by New “Cryobiopsy” Technique....Pages 249-253
Recent Development of In Vivo Cryotechnique to Cryobiopsy for Living Animals....Pages 255-259
Front Matter....Pages 261-261
Significance of Cryobiopsy for Morphological and Immunohistochemical Analyses of Xenografted Human Lung Cancer Tissues and Functional Blood Vessels....Pages 263-268
Immunolocalization of Serum Proteins in Xenografted Mouse Model of Human Tumor Cells....Pages 269-274
Histochemical Approach of Cryobiopsy for Glycogen Distribution in Living Mouse Livers Under Fasting and Local Circulation Loss Conditions....Pages 275-279
Front Matter....Pages 281-281
Bioimaging of Fluorescence-Labeled Mitochondria in Subcutaneously Grafted Murine Melanoma Cells by the “In Vivo Cryotechnique”....Pages 283-288
Application of “In Vivo Cryotechnique” to Visualization of ATP with Luciferin-Luciferase Reaction in Mouse Skeletal Muscles....Pages 289-292
Concluding Remarks....Pages 293-293
Back Matter....Pages 295-300