In Vitro Toxicity Indicators

Content: 
Methods in Toxicology, Page ii
Front Matter, Page iii
Copyright, Page iv
Dedication, Page v
Contributors, Pages xiii-xvii
Preface, Pages xix-xx, Charles A. Tyson, John M. Frazier
1 - Cellular and Molecular Pathobiology of Reversible and Irreversible Injury, Pages 1-12,12a,13-22, Benjamin F. Trump, Irene K. Berezesky
2 - Molecular Mechanisms of Toxic Cell Death: An Overview, Pages 23-32, Pierluigi Nicotera, Sten Orrenius
3 - Membrane Lipid Peroxidation: Cellular Mechanisms and Toxicological Implications, Pages 33-45, Lars Ernster, Paul Hochstein
4 - Enzyme Leakage as an Indicator of Cytotoxicity in Cultured Cells, Pages 46-49, Allison A. Welder, Daniel Acosta
5 - Cellular K+, Pages 50-57, V. Kefalas, N.H. Stacey
6 - Morphological and Biochemical Investigations on Plasma Membrane Blebbing during Cell Injury, Pages 58-71, Giorgio Bellomo, Francesca Mirabelli, Mariapia Vairetti, Francesca losi, Walter Malorni
7 - Morphological Evaluation of Gap Junctional Intercellular Communication, Pages 72-80, James E. Klaunig, Thomas K. Baker
8 - Fluorescence Microscopic Examination of Cytoskeletal Elements, Pages 81-95, Iih-Nan Chou, Wande Li, Glen B. Zamansky
9 - Energy Coupled Na+ Cotransport, Pages 97-107, Sue M. Ford
10 - Renal Organic Ion Transport, Pages 108-120, Carlotta E. Groves, Hilary V. Sheevers, Sharon J. McGuinness
11 - Endoplasmic Reticulum Calcium Pump, Pages 121-127, Leon Moore
12 - Measurement of Oxygen Consumption, Pages 128-139, Rick G. Schnellmann
13 - ATP and Other Energetic Parameters as Indicators of Cell Injury, Pages 140-151, Youngja Park, Tak Yee Aw, Dean P. Jones
14 - Evaluation of Glucose Metabolism in Vitro: Altered Competence of Intermediary Metabolism as an Index of Hepatotoxicity, Pages 152-163, Michael J. Olson
15 - Quantitation of DNA by Fluorometry, Pages 164-177, Sean R. Gallagher, Zbigniew Rymaszewski
16 - Techniques for Measuring Cell Proliferation, Pages 178-200, Frank Dolbeare, Martin Vanderlaan
17 - Nuclease P1-Enhanced 32P-Postlabeling Assay for Aromatic Carcinogen-DNA Adducts, Pages 201-215, M. Vijayaraj Reddy, Kurt Randerath
18 - Genomic Hypomethylation, Pages 216-227, Vincent L. Wilson
19 - DNA Degradation: Double-Strand Breaks, Pages 228-236, Andrew H. Wyllie, Mark J. Arends, Robert M. Hogg, Alistair Nunn
20 - Genotypic Mutation Analysis by RFLP/PCR, Pages 237-247, Fernando Aguilar, Peter Cerutti
21 - Gluconeogenesis in Liver Cells, Pages 248-257, Richard G. Knowles, Chris I. Pogson
22 - Measurement of Protein Synthesis, Pages 258-269, Michael J. Meredith
23 - Measuring Collagen Synthesis, Pages 270-278, William J. Lindblad
24 - Measurement of Macrophage Phagocytosis Using a Sheep Red Blood Cell Assay, Pages 279-286, Gregory L. Finch
25 - Cell Function Studies Using Fluorescent Ca2+ Indicators, Pages 287-314, Andrew P. Thomas
26 - Ca2+-Bioluminescent Indicators, Pages 315-327, André B. Borle
27 - Measurement of Receptor-Activated Accumulation of Inositol Phosphates as an Index of Phospholipase C Activity, Pages 328-339, Stephen B. Shears
28 - Covalent Binding to Proteins, Pages 340-348, Sidney D. Nelson
29 - Glutathione, Oxidized Glutathione, and Mixed Disulfides in Biological Samples, Pages 349-366, Evan P. Gallagher, Terrance J. Kavanagh, David L. Eaton
30 - Thiobarbituric Acid Assay Reactants, Pages 367-376, Steven D. Aust
31 - Volatile Hydrocarbons, Pages 377-383, Hermann Kappus
32 - Electronically Excited State Formation, Pages 384-399, Enrique Cadenas, Cecilia Giulivi, Fulvio Ursini, Alberto Boveris
33 - Spectrophotometric Detection of Lipid-Conjugated Dienes, Pages 400-406, Eric A. Glende Jr., Richard O. Recknagel
34 - Quantitation of Conjugated Dienes by a Diels-Alder Reaction, Pages 407-414, D.A.G. Mickle
35 - Quantitation of Conjugated Dienes by Second-Derivative UV Spectroscopy, Pages 415-420, F.P. Corongiu, S. Banni, B. Lombardi
36 - A CENTRIFUGE FILTRATION SEPARATION OF CELLS, AND THE MEASUREMENT OF CONSTITUENTS, Pages 421-430, Donald J. Reed
37 - Flow Cytometry, Pages 431-437, Jeffrey D. Laskin, Debra L. Laskin
38 - Single-Cell Microscopic Techniques for Studying Toxic Injury, Pages 438-455, John J. Lemasters, Anna-Liisa Nieminen, Enrique Chacon, John M. Bond, Ian Harper, Jeffrey M. Reece, Brian Herman
39 - Measurement of Macrophage and Neutrophil Chemotaxis, Pages 456-462, Debra L. Laskin
40 - Two-Dimensional Electrophoresis as Applied to Problems of in Vitro and in Vivo Toxicology, Pages 463-473, N.L. Anderson, R. Esquer-Blasco, N.G. Anderson
Index, Pages 475-497