Immunological Methods. Volume IV

Content: 
Front Matter, Page iii
Copyright, Page iv
Contributors, Pages xiii-xv
Preface, Pages xvii-xviii, Ivan Lefkovits
Contents of Previous Volumes, Pages xix-xxiv
Abbreviations List, Pages xxv-xxvii
1 - Solubilization of Cell Surface Membrane-Associated Molecules by Genetic Engineering, Pages 1-12, André Traunecker
2 - Methods and Vectors for Positive Selection and Cell-Free Expression of Lymphocyte cDNA Clones, Pages 13-33, Christopher Coleclough
3 - Application of Polymerase Chain Reaction in Molecular Immunology, Pages 35-60, Hans-Jürgen Thiesen, Giulia Casorati, Roland Lauster, Michael V. Wiles
4 - Target Detection Assay: A General Method to Determine DNA Binding Sites for Putative DNA-Binding Proteins, Pages 61-74, Hans-Jürgen Thiesen
5 - Analysis of cis- and trans-Acting Factors Regulating Gene Transcription, Pages 75-93, Richard H. Scheuermann
6 - Cell-Free System for Polyadenylation Using Mouse B Cell Extracts, Pages 95-105, Anders Virtanen, Una Chen
7 - In Vitro Transcriptional Rate Assay for Lymphoid Cells, Pages 107-119, Una Chen
8 - System for High-Level Production in Escherichia coli and Rapid Purification of Recombinant Proteins: Application to Epitope Mapping, Preparation of Antibodies, and Structure—Function Analysis, Pages 121-152, Dietrich Stüber, Hugues Matile, Gianni Garotta
9 - Analysis of Two-Dimensional Gel Electrophoretic Protein Patterns upon in Vivo Labeling of Mice, Pages 153-164, Gerd Pluschke, Ivan Lefkovits
10 - Biological Assay of Interleukins, Pages 165-174, Lucien Aarden, Maarten Helle
11 - Monoclonal Antibodies against Cytokine Receptors, Pages 175-185, Manfred Brockhaus, Hansruedi Loetscher
12 - Human Monoclonal Antibodies, Pages 187-195, Hubert Jacot-Guillarmod
13 - Isolation of Rat Immunoglobulin Class Switch Variants from Hybridomas by Sequential Sublining, Pages 197-213, Gerd Pluschke
14 - Mapping of T Cell Epitopes in Proteins: A Chemical Approach, Pages 215-219, Giampietro Corradin, Stéphane Demotz, Antonio Lanzavecchia
15 - Plasmodium Falciparum Malaria Parasite Cultures and Their Use in Immunology, Pages 221-234, Hugues Matile, J. Richard, L. Pink
16 - A Chemotactic Assay Enabling Recovery of Cells after Migration, Pages 235-249, Beat A. Imhof, Catherine Dargemont, Marie-Ange Deugnier, Dominique Dunon
17 - Assay for Quantitation of Cell-Cell Adhesion Using Fluorescent Dyes, Pages 251-263, Beat A. Imhof, Claude Schlienger, Klaus Handloser, Barbara Hesse, Roland H. Gisler
18 - Analysis of Avian T and B Lymphocytes, Pages 265-279, Olli Vainio, Antti Mansikka, Olli Lassila
19 - Isolation of Cells Involved in the Germinal Center Reaction: Germinal Center B Cells and Follicular Dendritic Cells, Pages 281-290, Marie H. Kosco, David Gray
20 - Applications of Fetal Thymus Organ Cultures in Studies of T Cell Development, Pages 291-309, Pawel Kisielow
21 - Transgenic Mice for Analysis of T Cell Development, Pages 311-325, Horst Bluethmann, Michael Steinmetz
Index, Pages 327-338