Immobilized Cells: Basics and Applications, Proceedings of an International Symposium organized under auspices of The Working Party on Applied Biocatalysis of the European Federation of Biotechnology Noordwijkerhout

Content: 
Preface
Pages v-vi

Acknowledgements
Page vii

Why immobilize? Original Research Article
Pages 3-16
C. Wandrey

Alginate gels — Some structure-function correlations relevant to their use as immobilization matrix for cells Original Research Article
Pages 19-30
B. Thu, O. Smidsrød, G. Skjak-Br˦k

Determination of biofilm diffusion coefficients using micro-electrodes Original Research Article
Pages 31-38
Evelien E. Beuling, Johannes C. Van den Heuvel, Simon P.P. Ottengraf

Complexity and heterogeneity of microenvironments in immobilised systems Original Research Article
Pages 39-46
J.E. Nava Saucedo, C. Roisin, J.-N. Barbotin

Fundamentals of dispersion in encapsulation technology Original Research Article
Pages 47-54
D. Poncelet, R.J. Neufeld

Stable support materials for the immobilization of viable cells Original Research Article
Pages 55-61
A. Muscat, U. Prüße, K.-D. Vorlop

Evaluation of density function of pore size distribution of calcium pectate hydrogel Original Research Article
Pages 62-69
M. Polakovič

Effects of formation conditions on size distribution of thermogel beads for cell immobilization Original Research Article
Pages 70-75
Cs. Sisak, T. Blickle, Zs. Ulbert, B. Szajáni

Calcium pectate gel could be a better alternative to calcium alginate gel in multiple applications of immobilized cells Original Research Article
Pages 76-83
P. Gemeiner, J. Nahálka, A. Vikartovská, J. Nahálková, M. Tomáška, E. àturdík, O. Markovič, A. Malovíková, I. Zatková, M. Ilavský

Adhesion of Lactococcus lactis diacetylactis to surfaces Original Research Article
Pages 84-89
S. Bourassa, J.-C. Vuillemard, P. Rouxhet

Immobilization of Arthrobacter globiformis 193 cells into PVA cryogel. Dehydrogenation of steroid substrates Original Research Article
Pages 90-97
V. Fokina, N. Suzina, A. Arinbasarova, A. Zubov, V. Lozinsky, K. Koshcheyenko

Screening of immobilization materials for anaerobic wastewater treatment Original Research Article
Pages 98-105
C.-S. Hwu, S.-K. Tseng

Ecologically pure process of acetate synthesis on diverse gaseous substrates by homoacetogenic bacteria, entrapped in POLY(VINYL ALCOHOL) CRYOGEL Original Research Article
Pages 106-111
A.M. Ryabokon, M.V. Kevbrina, M.A. Pusheva, A.L. Zubov, V.I. Lozinsky, E.I. Rainina

Entrapment of Zymomonas mobilis cells into PVA-Cryogel carrier in the presence of polyol cryoprotectants Original Research Article
Pages 112-117
V.I. Lozinsky, A.L. Zubov, T.A. Makhlis

Some microorganisms during their entrapment in Paag Act as “Biological accelerators” In how they affect the gel-formation rate Original Research Article
Pages 118-125
V.I. Lozinsky, A.S. Savvichev, B.L. Tumansky, D.I. Nikitin

Rhodococcus sp. immobilized by adsorption on chitin Original Research Article
Pages 126-131
G. Bianchi, L. Setti, G. Spagna, P.G. Pifferi

Immobilization of Aspergillus niger and Phanerochaete chrysosporium on polyurethane foam Original Research Article
Pages 132-135
A. Sanromán, G. Feijoo, J. M.Lema

Effect of C/N ratio and cellulose type on the cellulolytic activity of free and immobilized Trichoderma reesci Original Research Article
Pages 136-139
V. Jirku

Analysis of fungal β-glucanase system produced under conditions of mycelial immobilization Original Research Article
Pages 140-147
V. Jirku

Immobilization as a contact stimulation of yeast cell wall alterations Original Research Article
Pages 148-153
V. Jirku

Modelling the immobilisation of cells in a packed bed of porous carriers Original Research Article
Pages 154-161
Ronnie Willaert, Luc De Backer, Gino V. Baron

Porous silicone rubber as an immobilization matrix for microbial and mammalian cells : Natural immobilization of a mass transfer limited culture environment Original Research Article
Pages 162-172
A.J. Knights

Continuous fermentation by conventional and recombinant Saccharomyces cerevisiae immobilized in Ca-alginate beads hardened with trivalent ion Original Research Article
Pages 173-180
E. Roca, N. Meinander, M.J. Núñez, B. Hahn-Hägerdal, J.M. Lema

Encapsulation by polyelectrolyte complex formation — a way to make hepatocyte cultures safe, efficient and on-line available Original Research Article
Pages 181-188
H. Dautzenberg, J. Stange, S. Mitzner, B. Lukanoff

Influence of the microenvironment on immobilised Gibberella fujikuroi Original Research Article
Pages 189-195
C. Roisin, C. Bienaimé, J.E. Nava Saucedo, J.-N. Barbotin.

Local Mass Transfer Coefficients in Bacterial Biofilms Using Fluorescence Recovery After Photobleaching (FRAP) Original Research Article
Pages 196-204
James D. Bryers, F. Drummonc

Characteristics and selection criteria of support materials for immobilization of nitrifying bacteria Original Research Article
Pages 205-212
E.J.T.M. Leenen, V.A.P.M. Dos Santos, J. Tramper, R.H. Wijffels

Stability of alginate gels applied for cell entrapment in open systems Original Research Article
Pages 213-220
Christian Vogelsang, Kjetill Østgaard

Studies of cell viability of immobilized Chlamydomonas reinhardtii and glycerol photoproduction Original Research Article
Pages 221-228
R. León, J.A. Pizzano, F. Galván

Effect of drying medium on the viability of dried Lactobacillus helveticus CNRZ 303 immobilized in calcium-alginate beads Original Research Article
Pages 229-235
E. Selmer-Olsen, R. Pehrson, T. Sørhaug, S.-E. Birkeland

Comparison of retention and expression of recombinant plasmids between suspended and Biofilm-Bound bacteria degrading TCE Original Research Article
Pages 239-248
James D. Bryers, Robert R. Sharp

Immobilized-cell growth: Diffusion limitation in expanding micro-colonies Original Research Article
Pages 249-256
R.H. Wijffels, C.D. de Gooijer, A.W. Schepers, J. Tramper

Competition and cooperation of microorganisms in a coimmobilized aerobic/anaerobic mixed culture Original Research Article
Pages 257-263
G. John, Cs. Sisak, P. Komáromi, L. Hellendoom, K. Schügerl

Viability of immobilised cells: Use of specific ATP levels and oxygen uptake rates Original Research Article
Pages 264-271
P. Gikas, A.G. Livingston

Gelatin immobilized growing yeast cells: Changes in the glycosylation level of external invertase and cell wall composition Original Research Article
Pages 272-279
E. de Alteriis, J. Zueco, R. Sentandreu, P. Parascandola

Nitrification Activity of Immobilized Activated Sludge Evaluated by Respiration Rate Original Research Article
Pages 280-289
H. Nakamura, S. Miyabayashi, K. Noto, T. Sumino

Growth of immobilised cells: Results and predictions for membrane-attached biofilms using a novel in situ biofilm thickness measurement technique Original Research Article
Pages 290-297
L.M. Freitas dos Santos, P. Pavasant, E.N. Pistikopoulos, A.G. Livingston

Cryo-Electron microscopy of polymer particles in a high cell density synthetic biofilm Original Research Article
Pages 298-303
V. Thiagarajan, Y. Ming, L.E. Scriven, M.C. Flickinger

Investigation of Oxygen Consumption by E. coli Immobilized in a Synthetic Biofilm Using a Thin Film Plug Reactor (TFPR) Original Research Article
Pages 304-312
V. Thiagarajan, K.L. Swope, M.C. Flickinger

Investigation of Gene Expression in Synthetic Biofilms to Extend the Activity of Immobilized Whole Cell Catalysts Original Research Article
Pages 313-319
K.L. Swope, J. Liu, L.E. Scriven, J.L. Schottel, M.C. Flickinger

Screening and design of immobilized biocatalysts through the kinetic characterization by flow microcalorimetry Original Research Article
Pages 320-327
Peter Gemeiner, Vladimír àtefuca, Alica Welwardová-Vikartovská

Pitfalls of initial reaction rate measurements Original Research Article
Pages 328-335
M. Polakovič, G. Handriková, P. Ačai, V. àtefuca, V. Báleš

Growth and eruption of gel-entrapped microcolonies Original Research Article
Pages 336-340
Leonie E. Hüsken, Johannes Tramper, René H. Wijffels

Quantitative characterization of viability and growth dynamics of immobilized nitrifying cells Original Research Article
Pages 341-348
E.J.T.M. Leenen, A.A. Boogert, A.A.M. van Lammeren, J. Tramper, R.H. Wijffels

Modelling of the biotransformation from geraniol to nerol by freely suspended and immobilised grape (Vitis vinifera) cells Original Research Article
Pages 349-354
J. Guardiola, M. Cánovas, J.L. Iborra

Modelling and experimental validation of cell and substrate evolution in an immobilized system Original Research Article
Pages 355-361
C. Quirós, M. Rendueles, L.A. García, M. Díaz

Mass transfer limitations in a bioartificial pancreas Original Research Article
Pages 362-369
Ronnie Willaert, Gino V. Baron

Effect of dissolved oxygen concentration on pH-controlled fed- batch gluconate production by immobilised Aspergillus niger Original Research Article
Pages 370-378
M. Moresi, E. Parente, A. Ricciardi, M. Lanorte

Alginate-Immobilized thermotolerant yeast for conversion of cellulose to ethanol Original Research Article
Pages 379-383
N. Barron, D. Brady, G. Love, R. Marchant, P. Nigam, L. McHale, A.P. McHale.

Further research on polysaccharide production by immobilized cells of the li- chen Pseudevernia furfuracea (L.) Zopf. in polyacrylamide Original Research Article
Pages 384-389
Teresa Pereyra, C. Vicente

Study of parietin production by immobilized cells of Xanthoria parietina in calcium-alginate matrix Original Research Article
Pages 390-394
Teresa Pereyra, María del Carmen Molina, María Segovia, J.L. Mateos, C. Vicente

Effects of immobilization on polyol production by Pichia farinosa Original Research Article
Pages 395-401
B. Bisping, U. Baumann, R. Simmering

Bioprocess engineering considerations in cyclosporin A fermentation by immobilized fungus Tolypocladium inflatum Original Research Article
Pages 402-409
T.H. Lee, G.-T. Chun, Y.K. Chang, Jongdae Lee, S.N. Agathos

Nitrate uptake by immobilized growing Chlamydomonas reinhardtii cells Original Research Article
Pages 410-415
I. Garbayo, C. Braban, M.V. Lobato, C. Vflchez

Influence of the immobilization methodology in the stability and activity of P. putida UV4 Immobilized whole cells Original Research Article
Pages 416-423
J.V. Sinisterra, H. Dalton

Immobilization of the extremly thermophilic archaeon Pyrococcus furiosus in macro-porous carriers Original Research Article
Pages 424-430
Ralf Pörtner, Herbert Märkl

Immobilization of Mycobacterium sp. cells for sitosterol side chain cleavage in organic solvents Original Research Article
Pages 431-436
P. Fernandes, J.M.S. Cabrai, H.M. Pinheiro

Oxytetracycline production by free and immobilized cells of Streptomyces rimosus in batch and repeated batch cultures Original Research Article
Pages 437-443
H.A. El-Enshasy, M.A. Farid, A.I. El-Diwany

Bacterial conjugation within к-carrageenan gel beads: Biotic and abiotic factors affecting plasmid transfer Original Research Article
Pages 444-451
D.D.G. Mater, M. Craynest, J.-N. Barbotin, N. Truffaut, D. Thomas

Plasmid stability in immobilized Bacillus subtilis continuous cultures Original Research Article
Pages 452-457
M. Craynest, D. Mater, J.N. Barbotin, N. Truffaut, D. Thomas

Improved stability of a naturally occurring TOL plasmid in Pseudomonas putida by immobilization Original Research Article
Pages 458-463
M. Karbasi, E. Asilonu, T. Keshavarz

Strategies in the design of an enzymatic process for the synthesis of ampicillin: A whole cell E. coli recombinant penicillin amidase biocatalyst Original Research Article
Pages 464-471
S. Ospina, E. Barzana, O.T. Ramírez, A. López-Munguía

Immobilization of recombinant E. coli cells with phenol-lyase activity Original Research Article
Pages 472-478
S. Bielecki, R. Bolek

Kinetics of a co-immobilised system Original Research Article
Pages 479-485
L. Hellendoorn, J.C. van den Heuvel, S.P.P. Ottengraf

Dynamic modelling of an integrated nitrogen removal system using Co-immobilized microorganisms Original Research Article
Pages 486-493
Vitor A.P.M., Dos Santos, Johannes Tramper, René H. Wijffels

Scale-up aspects of immobilized cell reactors Original Research Article
Pages 497-504
J.J. Heijnen

Problems in Scale-up of Immobilized Cell Cultures Original Research Article
Pages 505-510
T. Keshavarz, C. Bucke, M.D. Lilly

Performance of a liquid-impelled loop reactor with immobilized cells Original Research Article
Pages 511-517
M.M.R. da Fonseca, D.M.R. Mateus, S.S. Alves

Effect of pulsation on morphology of Aspergillus niger and Phanerochaete chrysosporium in a fluidized-bed reactor Original Research Article
Pages 518-523
M.T. Moreira, G. Feijoo, A. Sanromán, J.M. Lema

The Estimation of the Optimum Amount of Solid Support in an Immobilized Cell Bioreactor Original Research Article
Pages 524-531
Simon D Goldfarb, Howard A.Chase

Double-chambered bioreactors based on plane immobilized-cell membrane structures Original Research Article
Pages 532-537
T. Lebeau, T. Jouenne, L. Mignot, G.-A. Junter

Continuous production and in situ extraction of isovaleraldehyde in a membrane bioreactor Original Research Article
Pages 538-545
F. Molinari, F. Aragozzini, J.M.S. Cabral, D.M.F. Prazeres

PEGASUS Innovative Biological Nitrogen Removal Process Using Entrapped Nitrifiers Original Research Article
Pages 546-555
H Emori, K Mikawa, M Hamaya, T Yamaguchi, K Tanaka, T Takeshima

Treatment of nitrogenous wastewaters by immobilized cyanobacteria in an airlift-fluidized photo-bioreactor Original Research Article
Pages 556-562
Chi Mei Lee, Chungsying Lu, Yi-Hsien Yin, Pei-Chung Chen

Photosynthetic bio-fuel cells using immobilized cyanobacterium Anabaena variabilis M-3 Original Research Article
Pages 563-569
Tatsuo Yagishita, Shigeki Sawayama, Ken-ichiro Tsukahara, Tomoko Ogi

Lactic acid fermentation using immobilized Lactobacillus casei cells Original Research Article
Pages 570-575
A. Senthuran, V. Senthuran, R. Kaul, B. Mattiasson

Lay-out of fixed bed reactor systems for effective production of biologicals with immobilized animal cells Original Research Article
Pages 576-582
Ralf Pörtner, Ines Lüdemann, Herbert Märkl

Industrial Application of Immobilized Biocatalysts in Japan Original Research Article
Pages 585-591
Takeji Shibatani

Enzyme production with immobilized filamentous fungi Original Research Article
Pages 592-599
Susan Linko, Reetta Haapala, Yi-Hong Zhu

Microbiological stability of an immobilized cell bioreactor with mixed lactic acid bacteria during continuous fermentation of milk Original Research Article
Pages 600-607
C. Lacroix, I. Sodini, G. Corrieu

Characterization of monoclonal IgA production and activity in hollow-fiber and fluidized-bed reactors Original Research Article
Pages 608-614
T.S. Stoll, P.-A. Ruffieux, E. Lüllau, U. von Stockar, I.W. Marison

Organoleptic profiles of different ciders after continuous fermentation (encapsulated living cells) versus batch fermentation (free cells) Original Research Article
Pages 615-621
J-P. Simon, A. Durieux, V. Pinnel, V. Garré, J. Vandegans, P. Rosseels, N. Godan, A.M. Plaisant, J-P. Defroyennes, G. Foroni

Application of nitrification by cells immobilized in polyethylene glycol Original Research Article
Pages 622-632
K. Tanaka, T. Sumino, H. Nakamura, T. Ogasawara, H. Emori

Immobilized cell technology in food processing Original Research Article
Pages 633-640
Claude P. Champagne

Bioencapsulation of carrot somatic embryos Original Research Article
Pages 641-648
J.-N. Barbotin, R. Timbert, C. Bazinet, D. Thomas

Alginate-polycation microcapsules for cell transplantation - Long time stability Original Research Article
Pages 649-654
B. Thu, O. Smidsrød, G. Skjak-Br˦k

Fumaric acid production by Rhizopus arrhizus immobilized on polyurethane sponge Original Research Article
Pages 655-660
F. Federici, M. Petruccioli

Application of immobilized yeast cells in the brewing industry Original Research Article
Pages 661-671
N.A. Mensour, A. Margaritis, C.L. Briens, H. Pilkington, I. Russell

Design and application of an immobilized loop bioreactor for continuous beer fermentation. Original Research Article
Pages 672-678
M. Andries, P.C. van Beveren, O. Goffin, C.A. Masschelein

Leuconostoc oenos entrapment: Application to continuous malo-lactic fermentation Original Research Article
Pages 679-686
A. Durieux, V. Garre, J. Mukamana, J.-M. Jourdain, D. Silva, A.-M. Plaisant, J.-P. Defroyennes, G. Foroni, J.-P. Simon

Practical Use of an Immobilized Cell Bioreactor for Continuous Prefermentation of Milk Original Research Article
Pages 687-694
Sodini I., Corrieu G., Lacroix C.

Behavior of Immobilized Nitrosomonas europaea and Paracoccus denitrificans in Tubular Gel for Nitrogen Removal in Wastewater Original Research Article
Pages 695-702
Hiroaki Uemoto, Hiroshi Saiki

Treatment of high strength ammonia wastewaters using immobilized biomass Original Research Article
Pages 703-709
W.M. Rostron, D.C. Stuckey, A.A. Young

Comparison of a system using immobilized microorganisms with a conventional activated sludge process for wastewater treatment Original Research Article
Pages 710-717
P. Chudoba, R. Pujol, H. Emori, J.C. Bourdelot, J.M. Rovel

Nitrification in PVAL beads: Influence of pH and temperature on nitrite oxidation Original Research Article
Pages 718-724
Th. Willke, K.-D. Vorlop

The concept of combined phosphorus and nitrogen removal by immobilized biomass Original Research Article
Pages 725-730
I. Wojnowska-Baryła, D. Stachowiak, E. Klimiuk

Anion-active surfactants degradation by immobilized cells Original Research Article
Pages 731-738
J. Húska, I. Závadská, M. Dobrotová, D. Tóth, P. Gemeiner, A. Vrbanová, J. Augustín

Entrapped microbial cell process for treatment of chlorophenolic compounds Original Research Article
Pages 739-744
Chi Mei Lee, Chih-Jen Lu, Chiou-Zong Huang, Chun-Chin Wang