Imaging and Spectroscopic Analysis of Living Cells: Live Cell Imaging of Cellular Elements and Functions

Content: 
Series Page
Page ii

Copryight Page
Page iv

Contributors
Pages xiii-xviii

Preface
Page xix
P. Michael Conn

Volume in Series
Pages xxi-l

Chapter one - Monitoring Cytoskeletal Dynamics in Living Neurons Using Fluorescence Photoactivation
Pages 3-21
Anne Gauthier-Kemper, Carina Weissmann, Hans-JГјrgen Reyher, Roland Brandt

Chapter two - Red/Far-Red Fluorescing DNA-Specific Anthraquinones for Nucl: Cyto Segmentation and Viability Reporting in Cell-Based Assays
Pages 23-45
Roy Edward

Chapter three - Methods to Measure Actin Treadmilling Rate in Dendritic Spines
Pages 47-58
Mikko Koskinen, Enni Bertling, Pirta Hotulainen

Chapter four - Live-Cell Imaging of Clathrin Coats
Pages 59-80
Comert Kural, Tom Kirchhausen

Chapter five - Imaging the Mitotic Spindle
Pages 81-103
Paul S. Maddox, Anne-Marie Ladouceur, Rajesh Ranjan, Jonas Dorn, Hery Ratsima, Damien D\'Amours, Amy S. Maddox

Chapter six - Modeling of Spatial Intracellular Signaling Events in Neurons
Pages 105-124
Wendy C. Wenderski, Susana R. Neves

Chapter seven - Imaging Chromosome Dynamics in Meiosis in Plants
Pages 125-143
Moira J. Sheehan, Wojciech P. Pawlowski

Chapter eight - Analyzing Lysosomes in Live Cells
Pages 145-157
Paul R. Pryor

Chapter nine - Detection and Quantification of Biomolecular Association in Living Cells using Single-Molecule Microscopy
Pages 159-186
Mario Brameshuber, Gerhard J. SchГјtz

Chapter ten - Visualizing Cardiac Ion Channel Trafficking Pathways
Pages 187-202
James W. Smyth, Robin M. Shaw

Chapter eleven - Live Cell Imaging of the Cytoskeleton
Pages 203-217
Eve G. Stringham, Nancy Marcus-Gueret, Laura Ramsay, Kristopher L. Schmidt

Chapter twelve - Fluorescence Single-Molecule Imaging of Actin Turnover and Regulatory Mechanisms
Pages 219-232
Naoki Watanabe

Chapter thirteen - Analysis of Cell Dispersion and Migration by Video-Microscopy
Pages 233-254
Arnaud Bonnomet, Christine Terryn, JГ©rГґme Cutrona, Antoine Jonquet, Philippe Birembaut, Jean-Marie Zahm

Chapter fourteen - Real Time Qualitative and Quantitative GLUT4 Translocation Assay
Pages 257-271
Maleppillil Vavachan Vijayakumar, Manoj Kumar Bhat

Chapter fifteen - Advanced Imaging of Cellular Signaling Events
Pages 273-289
Marek Cebecauer, Jana HumpolГ­ДЌkovГЎ, JerГ©mie Rossy

Chapter sixteen - Dynamic Imaging of Homo-FRET in Live Cells by Fluorescence Anisotropy Microscopy
Pages 291-327
Subhasri Ghosh, Suvrajit Saha, Debanjan Goswami, Sameera Bilgrami, Satyajit Mayor

Chapter seventeen - Time-Resolved Luminescence Resonance Energy Transfer Imaging of Protein–Protein Interactions in Living Cells
Pages 329-345
Harsha E. Rajapakse, Lawrence W. Miller

Chapter eighteen - Imaging of Corticosteroid Receptors in Live Cells
Pages 347-361
Mayumi Nishi

Chapter nineteen - Investigating Second Messenger Signaling In Vivo
Pages 363-382
RГјdiger Rudolf, Mathias Hafner, Marco Mongillo

Chapter twenty - Probes for Intracellular RNA Imaging in Live Cells
Pages 383-399
Philip J. Santangelo, Eric Alonas, Jeenah Jung, Aaron W. Lifland, Chiara Zurla

Chapter twenty-one - Imaging the Glycome in Living Systems
Pages 401-419
Boyangzi Li, Feiyan Mock, Peng Wu

Chapter twenty-two - Intracellular Magnesium Detection by Fluorescent Indicators
Pages 421-444
Valentina Trapani, Monika Schweigel-Röntgen, Achille Cittadini, Federica I. Wolf

Chapter twenty-three - Illuminating Mobile Zinc with Fluorescence: From Cuvettes to Live Cells and Tissues
Pages 445-468
Zhen Huang, Stephen J. Lippard

Chapter twenty-four - Quantitative Fluorescent Live Cell Imaging of Intracellular Ca2+ and H+ Ions in Malaria Parasites
Pages 469-483
Petra Rohrbach

Author Index
Pages 485-509

Subject Index
Pages 511-523