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ویرایش: 1
نویسندگان: P. Michael Conn (Eds.)
سری: Methods in Enzymology 168
ISBN (شابک) : 9780121820695
ناشر: Academic Press
سال نشر: 1989
تعداد صفحات: 940
زبان: English
فرمت فایل : PDF (درصورت درخواست کاربر به PDF، EPUB یا AZW3 تبدیل می شود)
حجم فایل: 26 مگابایت
در صورت تبدیل فایل کتاب Hormone Action Part K: Neuroendocrine Peptides به فرمت های PDF، EPUB، AZW3، MOBI و یا DJVU می توانید به پشتیبان اطلاع دهید تا فایل مورد نظر را تبدیل نمایند.
توجه داشته باشید کتاب عمل هورمون قسمت K: پپتیدهای نورواندوکرین نسخه زبان اصلی می باشد و کتاب ترجمه شده به فارسی نمی باشد. وبسایت اینترنشنال لایبرری ارائه دهنده کتاب های زبان اصلی می باشد و هیچ گونه کتاب ترجمه شده یا نوشته شده به فارسی را ارائه نمی دهد.
جلد 168 از روشها در آنزیمولوژی نه تنها برای کسانی که در این زمینه هستند، بلکه برای کسانی که در رشتههای مرتبط هستند که مطالعات خود را به طور نزدیک با علوم اعصاب مرتبط میدانند، ارزشمند خواهد بود. روشهایی ارائه شدهاند تا امکان انطباق آسان با سیستمهای جدید و تاکید بر کاربرد کلی و محدودیتهای بالقوه آنها را فراهم کنند
Volume 168 of Methods in Enzymology will prove invaluable not only to those in the field but also to those in related disciplines who find their studies becoming closely linked to the neurosciences. Methodologies are presented to allow easy adaptation to new systems and to stress their general applicability and potential limitations
Content:
Contributors to volume 168
Pages xi-xvi
Preface
Page xvii
P.Michael Conn
Volumes in series
Pages xix-xxxii
Strategy and methodology for development of antisera against procholecystokinin Original Research Article
Pages 3-7
Margery C. Beinfeld, Thomas L. O'Donohue
High-density multiple antigen-peptide system for preparation of antipeptide antibodies Original Research Article
Pages 7-15
James P. Tam
Preparation and use of complementary peptides Original Research Article
Pages 16-28
Kenneth L. Bost, J.Edwin Blalock
Fluorescent and cytotoxic analogs of corticotropin-releasing factor: Probes for studying target cells in heterogeneous populations Original Research Article
Pages 29-44
Jeffrey Schwartz, Wylie Vale
Cell-to-cell communication in peptide target cells of anterior pituitary Original Research Article
Pages 47-72
Carl Denef, Philippe Maertens, Wilfried Allaerts, Annick Mignon, Wim Robberecht, Luc Swennen, Peter Carmeliet
Application of fast atom bombardment mass spectrometry to posttranslational modifications of neuropeptides Original Research Article
Pages 73-103
P.C. Andrews, Jack E. Dixon
Electrophysiological analysis of opioid peptides: Extracellular and intracellular approaches Original Research Article
Pages 103-129
Edward D. French, John T. Williams
Dose-response testing of peptides by hippocampal brain slice recording Original Research Article
Pages 129-144
M.Ian Phillips, Reinhard A. Palovcik
Patch clamp recording from anterior pituitary cells identified by reverse hemolytic plaque assay Original Research Article
Pages 144-166
Thomas L. Croxton, William McD. Armstrong, Nira Ben-Jonathan
Microdialysis for measurement of neuroendocrine peptides Original Research Article
Pages 166-181
Jon E. Levine, Keith D. Powell
Use of microdialysis in neuroendocrinology Original Research Article
Pages 182-205
Keith M. Kendrick
In vitro perifusion of human hypothalamic and pituitary tissue Original Research Article
Pages 206-218
Dennis D. Rasmussen
Transient permeabilization of endocrine cells: Inositol lipid metabolism Original Research Article
Pages 218-225
Irene Winicov, Marvin C. Gershengorn
Cell cracking: Permeabilizing cells to macromolecular probes Original Research Article
Pages 225-233
Thomas F.J. Martin
Microsurgical techniques for studying functional correlates of hypothalamohypophyseal axis Original Research Article
Pages 234-254
Ichiro Murai, Walter C. Low, Nira Ben-Jonathan
Superfusion and static culture techniques for measurement of rapid changes in prolactin secretion Original Research Article
Pages 254-263
Gonzalo MartГnez de la Escalera, Karen C. Swearingen, Richard I. Weiner
Intracellular calcium levels in rat anterior pituitary cells: Single-cell techniques Original Research Article
Pages 263-284
Denis A. Leong
Use of protein kinase C-depleted cells for investigation of the role of protein kinase C in stimulus-response coupling in the pituitary Original Research Article
Pages 287-301
Craig A. McArdle, P.Michael Conn
Purification of neuropeptides: Cholecystokinin and vasoactive intestinal peptide Original Research Article
Pages 302-308
John Eng, Rosalyn S. Yalow
Purification and characterization of insulin-like growth factor-II receptors Original Research Article
Pages 309-324
Carolyn D. Scott, Robert C. Baxter
Measurement of hormone secretion from individual cells by cell blot assay Original Research Article
Pages 327-338
Marcia E. Kendall, W.C. Hymer
Measurement of phosphoinositide hydrolysis in isolated cell membrane preparations Original Research Article
Pages 338-347
Millie M. Chien, John C. Cambier
A rapid method for the resolution of protein kinase C subspecies from rat brain tissue Original Research Article
Pages 347-351
Mark S. Shearman, Kouji Ogita, Ushio Kikkawa, Yasutomi Nishizuka
Assay of peptidylglycine monooxygenase: Glycine-directed amidating enzyme Original Research Article
Pages 351-358
Thomas Brock, Jane Humm, J.S. Kizer
Assay of glutaminylpeptide cyclase Original Research Article
Pages 358-365
Jeanne B. Koger, Jane Humm, John S. Kizer
Primary thyrotropin-releasing hormone-degrading enzymes Original Research Article
Pages 365-371
Charles H. Emerson
Quantification of the mass of tyrosine monooxygenase in the median eminence and superior cervical ganglion Original Research Article
Pages 371-385
John C. Porter, Paulus S. Wang, Wojciech Kedzierski, Hector A. Gonzalez
Measurement of vasopressin-converting aminopeptidase activity and vasopressin metabolites Original Research Article
Pages 385-397
J.P.H. Burbach, Bin Liu
Quantitation of vasopressin and oxytocin mRNA levels in the brain Original Research Article
Pages 398-413
Hubert H.M. Van Tol, J.Peter H. Burbach
Granulosa cell aromatase bioassay for follicle-stimulating hormone Original Research Article
Pages 414-422
Kristine D. Dahl, Xiao-Chi Jia, Aaron J.W. Hsueh
Neuroendocrine regulation of oocyte tissue plasminogen activator Original Research Article
Pages 422-430
Thomas A. Bicsak, Carla M. Hekman, Aaron J.W. Hsueh
Assessment of peptide regulation of the autonomic nervous system Original Research Article
Pages 431-443
Marvin R. Brown, Roberta Allen, Laurel A. Fisher
Recognition, purification, and structural elucidation of mammalian physalaemin-related molecules Original Research Article
Pages 444-462
Lawrence H. Lazarus, William E. Wilson
Radioligand-binding assays for study of neurotensin receptors Original Research Article
Pages 462-481
Michel Goedert
Characterization of receptors for bombesin/gastrin-releasing peptide in human and murine cells Original Research Article
Pages 481-493
Terry W. Moody, Richard M. Kris, Gary Fiskum, Carol D. Linden, M. Berg, Joseph Schlessinger
Isolation and identification of neuroendocrine peptides from milk Original Research Article
Pages 494-497
Eli Hazum
Passive immunoneutralization: A method for studying the regulation of basal and pulsatile hormone secretion Original Research Article
Pages 498-516
Michael D. Culler, AndrГ©s Negro-Vilar
Combined antibody-high-performance liquid chromatography approach to assess prohormone processing Original Research Article
Pages 517-545
W. Wetsel, A. Negro-Vilar
Chromatographic methods for characterization of angiotensin in brain tissue Original Research Article
Pages 545-566
Klaus Hermann, Mohan K. Raizada, M.Ian Phillips
Measurement of thyrotropin-releasing hormone and its metabolites Original Research Article
Pages 567-573
Charles H. Emerson
Monoclonal antibodies: Uses in studies on vasopressin Original Research Article
Pages 574-587
Guy Valiquette, Simon Neubort
Detection and purification of inhibin using antisera generated against synthetic peptide fragments Original Research Article
Pages 588-617
Joan M. Vaughan, Jean Rivier, Anne Z. Corrigan, Richard McClintock, Carolyn A. Campen, Diane Jolley, Josef K. Voglmayr, C.Wayne Bardin, Catherine Rivier, Wylie Vale
Regulation of neuroendocrine peptide gene expression Original Research Article
Pages 618-633
M. Blum
Solution hybridization-nuclease protection assays for sensitive detection of differentially spliced substance P- and neurokinin A-encoding messenger ribonucleic acids Original Research Article
Pages 634-652
James E. Krause, Jean D. Cremins, Mark S. Carter, Elaine R. Brown, Margaret R. MacDonald
Quantifying carrier-mediated transport of peptides from the brain to the blood Original Research Article
Pages 652-660
William A. Banks, Abba J. Kastin
Characterizing molecular heterogeneity of gastrin-releasing peptide and related peptides Original Research Article
Pages 660-677
Joseph R. Reeve Jr., John H. Walsh
Neuropeptide gene transcription in central and peripheral nervous system tissue by nuclear run-on assay Original Research Article
Pages 681-690
Jeffrey D. White, Edmund F. LaGamma
Assaying the reporter gene chloramphenicol acetyltransferase Original Research Article
Pages 690-701
David W. Crabb, Carolyn D. Minth, Jack E. Dixon
In situ hybridization histochemical detection of neuropeptide mRNA using DNA and RNA probes Original Research Article
Pages 702-710
W.Scott Young III
Use of metal complexes in neuroendocrine studies Original Research Article
Pages 710-715
Ayalla Barnea
Methods for the study of somatostatin Original Research Article
Pages 716-729
M.C. Aguila, S.M. McCann
Mapping of gonadotropin-releasing hormone-receptor binding site using selective chemical modifications Original Research Article
Pages 729-737
Eli Hazum
In situ hybridization: Quantitation using radiolabeled hybridization probes Original Research Article
Pages 741-752
George R. Uhl
Nonradioactive methods of in situ hybridization: Visualization of neuroendocrine mRNA Original Research Article
Pages 753-761
P.C. Emson, H. Arai, S. Agrawal, C. Christodoulou, M.J. Gait
High-resolution in situ hybridization histochemistry Original Research Article
Pages 761-777
Frank Baldino Jr., Marie-Francoise Chesselet, Michael E. Lewis
In situ hybridization histochemistry combined with markers of neuronal connectivity Original Research Article
Pages 778-791
James S. Schwaber, Bibie M. Chronwall, Michael E. Lewis
Cytochemical techniques for studying the diffuse neuroendocrine system Original Research Article
Pages 791-808
A.E. Bishop, J.M. Polak
Quantitation and digital representation of in situ hybridization histochemistry Original Research Article
Pages 808-821
Michael E. Lewis, Wade T. Rogers, Rudolph G. Krause II, James S. Schwaber
Graphical and statistical approaches to data analysis for in situ hybridization Original Research Article
Pages 822-848
Joseph T. McCabe, Robert A. Desharnais, Donald W. Pfaff
Author index
Pages 849-881
Subject index
Pages 883-906