HLA from Benchtop to Bedside

Front-Matter_2021_HLA-from-Benchtop-to-Bedside
	HLA from Benchtop to Bedside
Copyright_2021_HLA-from-Benchtop-to-Bedside
	Copyright
Preface_2021_HLA-from-Benchtop-to-Bedside
	Preface
Acknowledgments_2021_HLA-from-Benchtop-to-Bedside
	Acknowledgments
Chapter-1---Introduction-and-some-history_2021_HLA-from-Benchtop-to-Bedside
	1 . Introduction and some history
		Fundamentals: innate and adaptive immunity
		References
Chapter-2---The-structure-of-the-major-histocompatibil_2021_HLA-from-Benchto
	2 . The structure of the major histocompatibility complex and major HLA components
		HLA class I
		Function of HLA Class Ia molecules
		HLA Ib molecules
		Other HLA class I histocompatibility genes: MICA and MICB
		HLA class II
		References
Chapter-3---Nomenclature-and-naming-conventions_2021_HLA-from-Benchtop-to-Be
	3 . Nomenclature and naming conventions for HLA
		HLA allele naming
		References
Chapter-4---The-crossmatch_2021_HLA-from-Benchtop-to-Bedside
	4 . The crossmatch
		Microcytotoxicity and the ``NIH standard'' technique
		Antihuman globulin
		Crossmatching by microcytotoxicity
		ABO and solid organ transplantation
		T and B cell isolation
		Immunofluorescence
		Flow cytometry
		HLA antibody detection in tissues
		References
		Further reading
Chapter-5---HLA-antibody-detection--screening--an_2021_HLA-from-Benchtop-to-
	5 . HLA antibody detection (screening) and identification
		Antibody identification by microlymphocytotoxicity
		Interpretation of antibody specificity in cell-based assays
		Panel reactive antibody
		Solid-phase antibody screening and identification
		Commercial solid-phase HLA antibody assays are qualitative, not quantitative
		Clinical indications for HLA antibody testing
		Single antigen bead result interpretation in clinical practice
		MFI as a quantitative result
		Virtual crossmatch
		Management of antibody-mediated transplant rejection
		HLA in Transfusion Medicine and Blood Banking
			TRALI
			Platelet transfusion support
		References
Chapter-6---HLA-typing-by-serologic-methods_2021_HLA-from-Benchtop-to-Bedsid
	6 . HLA typing by serologic methods
		References
Chapter-7---HLA-typing-by-molecular-methods_2021_HLA-from-Benchtop-to-Bedsid
	7 . HLA typing by molecular methods
		Molecular HLA typing ``before PCR''
			Sequencing
		Oligonucleotide probing and endonuclease cutting
			Restriction fragment length polymorphism
		Polymerase chain reaction and the molecular revolution in clinical medicine
		Polymerase chain reaction sequence specific primers
		PCR-RFLP
		PCR-rSSO
		High resolution typing ambiguities in molecular HLA typing
		The challenge of null alleles
		rtPCR (aka qPCR, real-time PCR, and quantitative PCR)
		References
Chapter-8---Massively-parallel--next-generation-_2021_HLA-from-Benchtop-to-B
	8 . Massively parallel (next generation) sequencing
		NGS sequencing by Hybridization and Ligation
		Selection of molecular HLA typing methods
		References
Chapter-9---HLA-alleles--disease-associations--and-_2021_HLA-from-Benchtop-t
	9 . HLA alleles, disease associations, and HLA pharmacogenetics
		The mathematics of disease associations
		How do HLA alleles cause disease?
		References
Chapter-10---Engraftment-monitoring_2021_HLA-from-Benchtop-to-Bedside
	10 . Engraftment monitoring
		A little history
		Microsatellite DNA, VNTRs, STRs
		Engraftment monitoring
		Engraftment monitoring by capillary electrophoresis
		Alternative platforms for engraftment monitoring
		References
		Further reading
Index_2021_HLA-from-Benchtop-to-Bedside
	Index
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