High Content Screening: A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery

Front Matter ....Pages i-xiii
Front Matter ....Pages 1-1
 Applications and Caveats on the Utilization of DNA-Specific Probes in Cell-Based Assays  (Roy Edward)....Pages 3-19
 General Staining and Segmentation Procedures for High Content Imaging and Analysis  (Kevin M. Chambers, Bhaskar S. Mandavilli, Nick J. Dolman, Michael S. Janes)....Pages 21-31
 Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis  (Bhaskar S. Mandavilli, Robert J. Aggeler, Kevin M. Chambers)....Pages 33-46
 Cell-Based High Content Analysis of Cell Proliferation and Apoptosis  (Bhaskar S. Mandavilli, Michelle Yan, Scott Clarke)....Pages 47-57
 Tools to Measure Autophagy Using High Content Imaging and Analysis  (Nick J. Dolman, Brent A. Samson, Kevin M. Chambers, Michael S. Janes, Bhaskar S. Mandavilli)....Pages 59-71
Front Matter ....Pages 73-73
 Guidelines for Microplate Selection in High Content Imaging  (Oscar J. Trask)....Pages 75-88
 Quality Control for High-Throughput Imaging Experiments Using Machine Learning in Cellprofiler  (Mark-Anthony Bray, Anne E. Carpenter)....Pages 89-112
 High-Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR, and Small Molecule Screening  (Steven A. Haney)....Pages 113-130
 Strategies and Solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays  (K. Kozak, B. Rinn, O. Leven, M. Emmenlauer)....Pages 131-148
 Live-Cell High Content Screening in Drug Development  (Milan Esner, Felix Meyenhofer, Marc Bickle)....Pages 149-164
 Challenges and Opportunities in Enabling High-Throughput, Miniaturized High Content Screening  (Debra Nickischer, Lisa Elkin, Normand Cloutier, Jonathan O’Connell, Martyn Banks, Andrea Weston)....Pages 165-191
Front Matter ....Pages 193-193
 Translocation Biosensors—Versatile Tools to Probe Protein Functions in Living Cells  (Verena Fetz, Roland H. Stauber, Shirley K. Knauer)....Pages 195-210
 High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions  (Yun Hua, Daniel P. Camarco, Christopher J. Strock, Paul A. Johnston)....Pages 211-227
 High Content Imaging Assays for IL-6-Induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines  (Paul A. Johnston, Malabika Sen, Yun Hua, Daniel P. Camarco, Tong Ying Shun, John S. Lazo et al.)....Pages 229-244
 Single Cell and Population Level Analysis of HCA Data  (David Novo, Kaya Ghosh, Sean Burke)....Pages 245-266
 Utilization of Multidimensional Data in the Analysis of Ultra-High-Throughput High Content Phenotypic Screens  (Judith Wardwell-Swanson, Yanhua Hu)....Pages 267-290
Front Matter ....Pages 291-291
 High Content Screening of Mammalian Primary Cortical Neurons  (Dario Motti, Murray Blackmore, John L. Bixby, Vance P. Lemmon)....Pages 293-304
 Human-Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications  (Joshua A. Harrill)....Pages 305-338
 Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High Content Assays  (Oksana Sirenko, Evan F. Cromwell)....Pages 339-354
 The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-Well Ultra-Low Attachment Microplates  (David A. Close, Daniel P. Camarco, Feng Shan, Stanton J. Kochanek, Paul A. Johnston)....Pages 355-369
 An Endothelial Cell/Mesenchymal Stem Cell Coculture Cord Formation Assay to Model Vascular Biology In Vitro  (Michelle Swearingen, Beverly Falcon, Sudhakar Chintharlapalli, Mark Uhlik)....Pages 371-382
 High-Throughput Automated Chemical Screens in Zebrafish  (Manush Saydmohammed, Michael Tsang)....Pages 383-393
Back Matter ....Pages 395-397