Hepatitis C Virus Protocols

Front Matter ....Pages i-xix
Front Matter ....Pages 1-1
 Overview of Direct-Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus  (Darrick K. Li, Raymond T. Chung)....Pages 3-32
 Similarities and Differences Between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV  (Laura Riva, Jean Dubuisson)....Pages 33-45
 Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR)  (Yun Zhang, Christian Zmasek, Guangyu Sun, Christopher N. Larsen, Richard H. Scheuermann)....Pages 47-69
Front Matter ....Pages 71-71
 Establishment of Replication-Competent HCV Strain with Minimum Modifications  (Takanobu Kato, Asako Murayama, Takaji Wakita)....Pages 73-83
 Full-Length Open Reading Frame Amplification of Hepatitis C Virus  (Ulrik Fahnøe, Jens Bukh)....Pages 85-91
 InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones  (Barnabas King, Richard Urbanowicz, Alexander W. Tarr, Jonathan K. Ball, C. Patrick McClure)....Pages 93-104
 Production and Purification of Cell Culture Hepatitis C Virus  (Cynthia de la Fuente, Maria Teresa Catanese)....Pages 105-119
 Embryonic or Induced Pluripotent Stem Cell-Derived Hepatocellular Systems for HCV Culture  (Xianfang Wu, Viet Loan Dao Thi)....Pages 121-135
Front Matter ....Pages 137-137
 Molecular Identification of Transmitted/Founder Hepatitis C Viruses and Their Progeny by Single Genome Sequencing  (Hui Li, Muhammad N. Zahid, Shuyi Wang, George M. Shaw)....Pages 139-155
 A Cell Culture Model for Persistent HCV Infection  (Victoria Castro, Ginés Ávila-Pérez, Lidia Mingorance, Pablo Gastaminza)....Pages 157-168
 Expression Cloning of Host Factors Required for the HCV Replication Cycle  (Sandra Ciesek, Thomas von Hahn)....Pages 169-182
 Application of Deep Mutational Scanning in Hepatitis C Virus  (Nicholas C. Wu, Hangfei Qi)....Pages 183-190
 Studying the Hepatitis C Virus-Induced Epigenetic Signature After Cure with Direct-Acting Antivirals  (Shira Perez, Meital Gal-Tanamy)....Pages 191-207
 Measuring Hepatitis C Virus Envelopment by Using a Proteinase K Protection Assay  (Allison E. Roder, Stacy M. Horner)....Pages 209-217
 Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells  (Solène Denolly, François-Loïc Cosset, Natalia Freitas)....Pages 219-234
 Functional Characterization of Apolipoproteins in the HCV Life Cycle  (Luhua Qiao, Guangxiang (George) Luo)....Pages 235-246
 Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy  (Pedro Matos Pereira, Caron Jacobs, Joe Grove)....Pages 247-261
 Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes  (Yasmine Baktash, Glenn Randall)....Pages 263-274
 Cloning and Analysis of Authentic Patient-Derived HCV E1/E2 Glycoproteins  (Richard A. Urbanowicz, Jonathan K. Ball, Alexander W. Tarr)....Pages 275-294
 Simultaneous Quantification of Hepatitis C Virus Envelope Glycoproteins E1 and E2 by Dual-Color Fluorescence Immunoblot Analysis  (Fang Chen, Erick Giang, Radhika Gopal, Mansun Law)....Pages 295-304
 Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells  (Samantha A. Yost, Jillian Whidby, Abdul G. Khan, Yuanyuan Wang, Joseph Marcotrigiano)....Pages 305-316
Front Matter ....Pages 317-317
 Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses Upon Cell–Cell Contact  (Séverin Coléon, Sonia Assil, Marlène Dreux)....Pages 319-335
 Complement Regulation and Immune Evasion by Hepatitis C Virus  (Young-Chan Kwon, Ranjit Ray)....Pages 337-347
 Direct Ex Vivo Functional Analysis of HCV-Specific T Cells  (Su-Hyung Park, Eui-Cheol Shin)....Pages 349-361
 Simultaneous Analysis of HCV-Specific CD4+ and CD8+ T Cells by Multicolor Flow Cytometry  (David Wolski, Georg M. Lauer)....Pages 363-380
 Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology  (Erick Giang, Fernando Aleman, Mansun Law)....Pages 381-393
 Isolation of HCV Neutralizing Antibodies by Yeast Display  (Zhen-yong Keck, Yong Wang, Patrick Lau, Steven K. H. Foung)....Pages 395-419
 Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA  (Marian Major, Mansun Law)....Pages 421-432
 In Vitro Neutralization Assay Using Cultured Hepatitis C Virus  (Jannick Prentoe, Jens Bukh)....Pages 433-439
 Standardized Method for the Study of Antibody Neutralization of HCV Pseudoparticles (HCVpp)  (Justin R. Bailey, Richard A. Urbanowicz, Jonathan K. Ball, Mansun Law, Steven K. H. Foung)....Pages 441-450
Front Matter ....Pages 451-451
 Detection of Antigen-Specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice  (Dustin A. Cobb, Aditya P. Dandekar, Young S. Hahn)....Pages 453-458
 Mice with Chimeric Human Livers and Their Applications  (Donna N. Douglas, Norman M. Kneteman)....Pages 459-479
 Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies  (Jenna M. Gaska, Qiang Ding, Alexander Ploss)....Pages 481-503
Back Matter ....Pages 505-508