Handbook for Rhizobia: Methods in Legume-Rhizobium Technology

Front Matter....Pages i-xvi
Front Matter....Pages 1-6
Collecting Nodules and Isolating Rhizobia....Pages 7-23
Observing the Infection Process....Pages 24-30
Cultural Properties, Cell Morphology, and Nutritional Requirements of Rhizobia....Pages 31-37
Demonstrating Genetic Diversity in Rhizobia Using Patterns of Carbohydrate Utilization and Intrinsic Antibiotic Resistance....Pages 38-46
Quantifying the Growth of Rhizobia....Pages 47-57
Counting Rhizobia by a Plant Infection Method....Pages 58-64
Counting Serologically Specific Rhizobia in Soil and Peat Inoculants Using Membrane Filters and Immunofluorescence....Pages 65-74
Back Matter....Pages 75-77
Front Matter....Pages 79-88
Developing Antisera....Pages 89-93
Somatic Agglutination Reactions with Pure Cultures of Rhizobia....Pages 94-101
Agglutinating Antigens from Root Nodules....Pages 102-106
Performing Rhizobial Antigen-Antibody Reactions by Gel Immunodiffusion....Pages 107-111
Determining Strain Occupancy in Soybean Nodules by Gel Immunodiffusion....Pages 112-119
Producing and Applying Fluorescent Antibodies....Pages 120-130
Identifying Rhizobia by the Indirect Enzyme-Linked Immunosorbent Assay....Pages 131-139
Identifying Rhizobia by Immunoblot....Pages 140-148
Isolating Spontaneous Antibiotic-Resistant Mutants of Rhizobia....Pages 149-152
Analyzing Nodule Occupancy Using Antibiotic-Resistant Markers....Pages 153-157
Distinguishing between Strains of Rhizobia by Rhizobiophage Susceptibility....Pages 158-162
Back Matter....Pages 163-164
Front Matter....Pages 165-169
Testing For Genetic Compatibility between Rhizobia and Legumes....Pages 171-176
Screening Rhizobia for Nitrogen-Fixation Potential....Pages 177-181
Back Matter....Pages 214-215
Front Matter....Pages 165-169
Screening Effective Strains of Rhizobia in Potted Field Soil....Pages 182-188
Verifying the Nitrogen-Fixing Potential of Glasshouse-Selected Soybean Rhizobia in the Field Environment....Pages 189-197
Evaluating the Symbiotic Potential of Indigenous Rhizobial Populations of Soils Using the Whole-Soil Inocula Technique....Pages 198-205
Investigating the Importance of Optimal Soil Fertility in the Response of a Legume to Inoculation with Rhizobia....Pages 206-213
Back Matter....Pages 214-215
Front Matter....Pages 217-223
Producing Broth Cultures in Simple Glass Fermentors....Pages 225-231
Producing Inoculum in a Steel Fermentor....Pages 232-239
Preparing a Range of Carrier Materials and Producing Inoculants....Pages 240-248
Preparing Inoculants Using Diluted Cultures of Rhizobia and Presterilized Peat....Pages 249-258
Testing the Survival of Rhizobia on Inoculated Seeds....Pages 259-264
Back Matter....Pages 265-266
Front Matter....Pages 267-272
Analyzing Plasmid Profiles of Rhizobium spp. by a Modified Eckhardt Vertical Gel Electrophoresis Procedure....Pages 273-278
Isolating and Purifying Genomic DNA of Rhizobia Using a Large-Scale Method....Pages 279-283
Isolating and Purifying Genomic DNA of Rhizobia Using a Rapid Small-Scale Method....Pages 284-288
Digesting Genomic DNA of Rhizobia with Restriction Endonucleases....Pages 289-292
Separating Restriction Fragments of Genomic DNA by Horizontal Agarose Gel Electrophoresis....Pages 293-297
Transferring Electrophoretically Separated DNA from Agarose Gels to a Membrane by Southern Blotting....Pages 298-302
Preparing a DNA Probe for Detecting the nif Genes on Symbiotic Plasmids of Rhizobium spp.....Pages 303-309
Incorporating a Nonradioactive Label into a DNA Probe by Nick Translation....Pages 310-312
Using a Nonradioactively Labeled nif KDH Gene Probe to Locate Complementary Sequences of Rhizobial DNA Immobilized on Membranes....Pages 313-317
Back Matter....Pages 318-319
Back Matter....Pages 321-450