Guide to Yeast Genetics and Molecular Biology

Content: 
Contributors to volume 194
Pages xi-xvi

Preface
Page xvii
Christine Guthrie, Gerald R. Fink

Volumes in series
Pages xix-xxxiv

Process guide
Pages xxxv-xxxvii

[1] Getting started with yeast Original Research Article
Pages 3-21
Fred Sherman

[2] Micromanipulation and dissection of asci Original Research Article
Pages 21-37
Fred Sherman, James Hicks

[3] Mapping yeast genes Original Research Article
Pages 38-57
Fred Sherman, Paul Wakem

[4] Positional mapping of genes by chromosome blotting and chromosome fragmentation Original Research Article
Pages 57-77
Sandra L. Gerring, Carla Connelly, Philip Hieter

[5] Assay of yeast mating reaction Original Research Article
Pages 77-93
George F. Sprague Jr.

[6] Monitoring meiosis and sporulation in Saccharomyces cerevisiae Original Research Article
Pages 94-110
Yona Kassir, Giora Simchen

[7] Identifying sporulation genes, visualizing synaptonemal complexes, and large-scale spore and spore wall purification Original Research Article
Pages 110-131
Rochelle Easton Esposito, Michael Dresser, Michael Breitenbach

[8] Putting the HO gene to work: Practical uses for mating-type switching Original Research Article
Pages 132-146
Ira Herskowitz, Robert E. Jensen

[9] Spore enrichment Original Research Article
Pages 146-149
Beth Rockmill, Eric J. Lambie, G.Shirleen Roeder

[10] Analysis and manipulation of yeast mitochondrial genes Original Research Article
Pages 149-165
Thomas D. Fox, Linda S. Folley, Julio J. Mulero, Thomas W. McMullin, Peter E. Thorsness, Lars O. Hedin, Maria C. Costanzo

[11] DNA of Saccharomyces cerevisiae Original Research Article
Pages 169-182
Peter Philippsen, Agathe Stotz, Christine Scherf

[12] High-efficiency transformation of yeast by electroporation Original Research Article
Pages 182-187
Daniel M. Becker, Leonard Guarente

[13] Transmission of plasmid DNA to yeast by conjugation with bacteria Original Research Article
Pages 187-195
Jack A. Heinemann, George F. Sprague Jr.

[14] Cloning genes by complementation in yeast Original Research Article
Pages 195-230
Mark D. Rose, James R. Broach

[15] Gene isolation with О»gt11 system Original Research Article
Pages 230-238
Richard A. Young, Ronald W. Davis

[16] Gene overexpression in studies of Saccharomyces cerevisiae Original Research Article
Pages 239-251
Jasper Rine

[17] Preparation of clone libraries in yeast artificial-chromosome vectors Original Research Article
Pages 251-270
David T. Burke, Maynard V. Olson

[18] Classical mutagenesis techniques Original Research Article
Pages 273-281
Christopher W. Lawrence

[19] Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast Original Research Article
Pages 281-301
Rodney Rothstein

[20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast Original Research Article
Pages 302-318
Robert S. Sikorski, Jef D. Boeke

[21] Recovery of plasmids from yeast into Escherichia coli: Shuttle vectors Original Research Article
Pages 319-329
Jeffrey N. Strathern, David R. Higgins

[22] Shuttle mutagenesis: Bacterial transposons for genetic manipulations in yeast Original Research Article
Pages 329-342
Merl F. Hoekstra, H.Steven Seifert, Jac Nickoloff, Fred Heffron

[23] Ty mutagenesis in Saccharomyces cerevisiae Original Research Article
Pages 342-361
David J. Garfinkel, Jeffrey N. Strathern

[24] Transformation of yeast directly with synthetic oligonucleotides Original Research Article
Pages 362-369
Richard P. Moerschell, Goutam Das, Fred Sherman

[25] Vectors for expression of cloned genes in yeast: Regulation, overproduction, and underproduction Original Research Article
Pages 373-388
Jane C. Schneider, Leonard Guarente

[26] Vectors for constitutive and inducible gene expression in yeast Original Research Article
Pages 389-398
Mark Schena, Didier Picard, Keith R. Yamamoto

[27] Preparation of high molecular weight RNA Original Research Article
Pages 398-405
Karl Köhrer, Horst Domdey

[28] Preparation and analysis of low molecular weight RNAs and small ribonucleoproteins Original Research Article
Pages 405-415
Jo Ann Wise

[29] Measurement of mRNA decay rates in Saccharomyces cerevisiae Original Research Article
Pages 415-423
Roy Parker, David Herrick, Stuart W. Peltz, Allan Jacobson

[30] Labeling of RNA and phosphoproteins in Saccharomyces cerevisiae Original Research Article
Pages 423-428
Jonathan R. Warner

[31] Tackling the protease problem in Saccharomyces cerevisiae Original Research Article
Pages 428-453
Elizabeth W. Jones

[32] Structural and functional analysis of yeast ribosomal proteins Original Research Article
Pages 453-477
H.A. RauГ©, W.H. Mager, R.J. Planta

[33] High-expression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors Original Research Article
Pages 477-490
T.J. Koerner, John E. Hill, Alan M. Myers, Alexander Tzagoloff

[34] Production of proteins by secretion from yeast Original Research Article
Pages 491-507
Donald T. Moir, Lance S. Davidow

[35] Epitope tagging and protein surveillance Original Research Article
Pages 508-519
Peter A. Kolodziej, Richard A. Young

[36] Reverse biochemistry: Methods and applications for synthesizing yeast proteins in vitro Original Research Article
Pages 520-535
Kevin Struhl

[37] In vitro protein synthesis Original Research Article
Pages 536-545
Michael J. Leibowitz, Francis P. Barbone, Denise E. Georgopoulos

[38] RNA polymerase II transcription in vitro Original Research Article
Pages 545-550
Neal F. Lue, Peter M. Flanagan, Raymond J. Kelleher III, Aled M. Edwards, Roger D. Kornberg

[49] Direct sequence and footprint analysis of yeast DNA by primer extension Original Research Article
Pages 550-562
Jon M. Huibregtse, David R. Engelke

[40] Immunofluorescence methods for yeast Original Research Article
Pages 565-602
John R. Pringle, Alison E.M. Adams, David G. Drubin, Brian K. Haarer

[41] Preparation of yeast cells for thin-section electron microscopy Original Research Article
Pages 602-608
Breck Byers, Loretta Goetsch

[42] Immunogold labeling of yeast ultrathin sections Original Research Article
Pages 608-626
Michael W. Clark

[43] Analysis of mitochondrial function and assembly Original Research Article
Pages 627-643
Michael P. Yaffe

[44] Methods for studying the yeast vacuole Original Research Article
Pages 644-661
Christopher J. Roberts, Christopher K. Raymond, Carl T. Yamashiro, Tom H. Stevens

[45] Analysis of polypeptide transit through yeast secretory pathway Original Research Article
Pages 662-674
Alex Franzusoff, Jonathan Rothblatt, Randy Schekman

[46] In vitro protein translocation across microsomal membranes of Saccharomyces cerevisiae Original Research Article
Pages 675-682
Pablo D. Garcia, William Hansen, Peter Walter

[47] Analysis of glycoproteins from Saccharomyces cerevisiae Original Research Article
Pages 682-697
P. Orlean, M.J. Kuranda, C.F. Albright

[48] Yeast endocytosis assays Original Research Article
Pages 697-710
Vjekoslav Dulic, Mark Egerton, Ibrahim Elguindi, Susan Raths, Birgit Singer, Howard Riezman

[49] Inducing and assaying heat-shock response in Saccharomyces cerevisiae Original Research Article
Pages 710-717
Charles M. Nicolet, Elizabeth A. Craig

[50] Nucleolar-specific positive stains for optical and electron microscopy Original Research Article
Pages 717-728
Michael W. Clark

[51] Staining of actin with fluorochrome-conjugated phalloidin Original Research Article
Pages 729-731
Alison E.M. Adams, John R. Pringle

[52] Staining of bud scars and other cell wall chitin with Calcofluor Original Research Article
Pages 732-735
John R. Pringle

[53] Isolation of yeast nuclei Original Research Article
Pages 735-749
John P. Aris, GГјnter Blobel

[54] Analysis of chromosome segregation in Saccharomyces cerevisiae Original Research Article
Pages 749-773
James H. Shero, Michael Koval, Forrest Spencer, Robert E. Palmer, Philip Hieter, Douglas Koshland

[55] Genetic screens and selections for cell and nuclear fusion mutants Original Research Article
Pages 774-792
Vivian Berlin, Julie A. Brill, Joshua Trueheart, Jef D. Boeke, Gerald R. Fink

[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe Original Research Article
Pages 795-823
Sergio Moreno, Amar Klar, Paul Nurse

[57] Genetic and physical maps of Saccharomyces cerevisiae Original Research Article
Pages 827-863
Robert K. Mortimer, David Schild, C.Rebecca Contopoulou, Jonathan A. Kans

Author index
Pages 865-903

Subject index
Pages 905-933