Guide to Research Techniques in Neuroscience

Front Cover
Guide to Research Techniques in Neuroscience
Copyright
Contents
Foreword to the Second Edition
Foreword to the First Edition
Preface to the Second Edition
Preface to the First Edition
Introduction
	LEVELS OF INVESTIGATION
	METHODS OF STUDYING THE NERVOUS SYSTEM
	UNDERSTANDING TECHNIQUES IN NEUROSCIENCE
Chapter 1 - Whole-Brain Imaging
	STRUCTURAL BRAIN IMAGING TECHNIQUES
	FUNCTIONAL BRAIN IMAGING TECHNIQUES
	FUNCTIONAL IMAGING EXPERIMENTAL DESIGN AND ANALYSIS
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
	Websites
Chapter 2 - Animal Behavior
	CONSIDERATIONS FOR CHOOSING AND PERFORMING A BEHAVIORAL ASSAY
	RODENT BEHAVIORAL PARADIGMS
	DROSOPHILA BEHAVIORAL PARADIGMS
	CAENORHABDITIS ELEGANS BEHAVIORAL PARADIGMS
	NONHUMAN PRIMATE BEHAVIORAL PARADIGMS
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books and Review Articles
	Primary Research Articles—Papers First Describing Standard Behavioral Assays
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
Chapter 3 - Stereotaxic Surgeries and In Vivo Techniques
	STEREOTAXIC SURGERIES
	IMPLANTS FOR LONG-TERM ACCESS TO THE BRAIN
	MANIPULATING NEURAL ACTIVITY IN VIVO
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
Chapter 4 - Electrophysiology
	A BRIEF REVIEW OF THE ELECTRICAL PROPERTIES OF NEURONS
	THE ELECTROPHYSIOLOGY RIG
	CATEGORIES OF ELECTROPHYSIOLOGY RECORDINGS
	ELECTROPHYSIOLOGY TISSUE PREPARATIONS
	MANIPULATING NEURAL ACTIVITY DURING ELECTROPHYSIOLOGY EXPERIMENTS
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
Chapter 5 - Microscopy
	ESSENTIAL PRINCIPLES OF MICROSCOPY
	LIGHT MICROSCOPY
	FLUORESCENCE MICROSCOPY
	ELECTRON MICROSCOPY
	PREPARING AND INTERPRETING MICROSCOPY DATA
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
	Websites
Chapter 6 - Visualizing Neural Structure
	TISSUE PREPARATION
	VISUALIZING MORPHOLOGY
	VISUALIZING GENE AND PROTEIN EXPRESSION
	VISUALIZING CIRCUITRY
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
	Websites
Chapter 7 - Visualizing Neural Function
	STATIC MARKERS OF ACTIVITY
	VISUALIZING NEURAL ACTIVITY
	VISUALIZING PROTEIN FUNCTION
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
Chapter 8 - Manipulating Neural Activity
	EXPERIMENTAL LOGIC OF MANIPULATING NEURAL ACTIVITY
	PHYSICAL MANIPULATION
	ELECTRICAL MANIPULATION
	PHARMACOLOGICAL MANIPULATION
	GENETIC MANIPULATION
	PHARMACOGENETIC MANIPULATION
	OPTOGENETIC MANIPULATION
	NEUROMODULATION TECHNIQUES USED IN HUMANS
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
	Websites
Chapter 9 - Identifying Genes and Proteins of Interest
	A BRIEF REVIEW OF GENES AND PROTEINS
	GENETIC SCREENS
	IN SILICO SCREENS
	MOLECULAR SCREENS
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
	Websites
Chapter 10 - Molecular Cloning and Recombinant DNA Technology
	ISOLATING DNA FRAGMENTS
	CLONING DNA
	IDENTIFYING DNA
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
	Websites
Chapter 11 - Gene Delivery Strategies
	PHYSICAL GENE DELIVERY
	CHEMICAL GENE DELIVERY
	VIRAL GENE DELIVERY
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
	Websites
Chapter 12 - Making and Using Transgenic Organisms
	TRANSGENES
	THE TRANSGENIC CONSTRUCT
	BINARY TRANSGENIC SYSTEMS
	MAKING TRANSGENIC ORGANISMS
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
	Websites
Chapter 13 - Manipulating Endogenous Genes
	CLASSICAL GENE TARGETING METHODS
	NUCLEASE-BASED GENE TARGETING METHODS
	DISRUPTING GENE PRODUCTS
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
Chapter 14 - Cell Culture Techniques
	CELL CULTURE EQUIPMENT AND REAGENTS
	IMMORTALIZED CELL LINES
	PRIMARY CELL AND TISSUE CULTURE
	STEM CELL CULTURES
	MANIPULATING CELLS IN CULTURE
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
	Websites
Chapter 15 - Biochemical Assays and Intracellular Signaling
	INTRODUCTION TO SIGNAL TRANSDUCTION AND INTRACELLULAR SIGNALING
	FUNDAMENTAL TOOLS USED TO STUDY PROTEINS
	INVESTIGATING PROTEIN EXPRESSION
	INVESTIGATING PROTEIN–PROTEIN INTERACTIONS
	INVESTIGATING POSTTRANSLATIONAL MODIFICATIONS
	INVESTIGATING PROTEIN–DNA INTERACTIONS
	CONCLUSION
	SUGGESTED READING AND REFERENCES
	Books
	Review Articles
	Primary Research Articles—Interesting Examples from the Literature
	Protocols
	Websites
Glossary
Index