Ghrelin

Content: 
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xv-xx

Preface
Page xxi
Masayasu Kojima, Kenji Kangawa

Methods in Enzymology
Pages xxiii-lvii

Chapter One - History to the Discovery of Ghrelin
Pages 3-32
Cyril Y. Bowers

Chapter Two - Orphan GPCRs and Methods for Identifying Their Ligands
Pages 33-44
Morikatsu Yoshida, Mikiya Miyazato, Kenji Kangawa

Chapter Three - Purification of Rat and Human Ghrelins
Pages 45-61
Masayasu Kojima, Hiroshi Hosoda, Kenji Kangawa

Chapter Four - Variety of Acyl Modifications in Mammalian Ghrelins
Pages 63-73
Takanori Ida

Chapter Five - Determination of Nonmammalian Ghrelin
Pages 75-87
Hiroyuki Kaiya, Hiroshi Hosoda, Kenji Kangawa, Mikiya Miyazato

Chapter Six - Morphological Analysis of Ghrelin Neurons in the Hypothalamus
Pages 91-99
Haruaki Kageyama, Fumiko Takenoya, Seiji Shioda

Chapter Seven - High-Performance Liquid Chromatography Analysis of Hypothalamic Ghrelin
Pages 101-112
Takahiro Sato, Masayasu Kojima

Chapter Eight - Standard Sample Collections for Blood Ghrelin Measurements
Pages 113-126
Hiroshi Hosoda, Kenji Kangawa

Chapter Nine - From Ghrelin to Ghrelin's O-Acyl Transferase
Pages 129-146
Jesus A. Gutierrez, Jill A. Willency, Michael D. Knierman, Tamer Coskun, Patricia J. Solenberg, Doug R. Perkins, Richard E. Higgs, John E. Hale

Chapter Ten - Enzymatic Characterization of GOAT, ghrelin O-acyltransferase
Pages 147-163
Hideko Ohgusu, Tomoko Takahashi, Masayasu Kojima

Chapter Eleven - The Study of Ghrelin Deacylation Enzymes
Pages 165-179
Motoyasu Satou, Hiroyuki Sugimoto

Chapter Twelve - Synthesis of Ghrelin: Chemical Synthesis and Semisynthesis for Large-Scale Preparation of Modified Peptides
Pages 183-203
Tomohiro Makino, Masaru Matsumoto, Yoshiharu Minamitake

Chapter Thirteen - Ghrelin O-Acyltransferase Assays and Inhibition
Pages 205-228
Martin S. Taylor, Yousang Hwang, Po-Yuan Hsiao, Jef D. Boeke, Philip A. Cole

Chapter Fourteen - Model-Based Evaluation of Growth Hormone Secretion
Pages 231-248
Johannes D. Veldhuis, Daniel M. Keenan

Chapter Fifteen - Ghrelin in the Control of Energy, Lipid, and Glucose Metabolism
Pages 249-260
Kristy M. Heppner, Timo D. Müller, Jenny Tong, Matthias H. Tschöp

Chapter Sixteen - Ghrelin and the Vagus Nerve
Pages 261-269
Yukari Date

Chapter Seventeen - Measurement of AMP-Activated Protein Kinase Activity and Expression in Response to Ghrelin
Pages 271-287
Chung Thong Lim, Francesca Lolli, Julia D. Thomas, Blerina Kola, MГЎrta Korbonits

Chapter Eighteen - Ghrelin and Gastrointestinal Movement
Pages 289-301
Naoki Fujitsuka, Akihiro Asakawa, Haruka Amitani, Mineko Fujimiya, Akio Inui

Chapter Nineteen - Ghrelin Acylation by Ingestion of Medium-Chain Fatty Acids
Pages 303-315
Yoshihiro Nishi, Hiroharu Mifune, Masayasu Kojima

Chapter Twenty - Islet ОІ-Cell Ghrelin Signaling for Inhibition of Insulin Secretion
Pages 317-331
Katsuya Dezaki, Toshihiko Yada

Chapter Twenty-One - Rikkunshito as a Ghrelin Enhancer
Pages 333-351
Hiroshi Takeda, Shuichi Muto, Koji Nakagawa, Shunsuke Ohnishi, Chiharu Sadakane, Yayoi Saegusa, Miwa Nahata, Tomohisa Hattori, Masahiro Asaka

Chapter Twenty-Two - Thermogenic Characterization of Ghrelin Receptor Null Mice
Pages 355-370
Ligen Lin, Yuxiang Sun

Chapter Twenty-Three - Transgenic Mice Overexpressing Ghrelin or Ghrelin Analog
Pages 371-377
Hiroyuki Ariyasu, Go Yamada, Hiroshi Iwakura, Takashi Akamizu, Kenji Kangawa, Kazuwa Nakao

Chapter Twenty-Four - Therapeutic Potential of Ghrelin in Restricting-Type Anorexia Nervosa
Pages 381-398
Mari Hotta, Rina Ohwada, Takashi Akamizu, Tamotsu Shibasaki, Kenji Kangawa

Chapter Twenty-Five - Clinical Application of Ghrelin for Chronic Respiratory Diseases
Pages 399-407
Nobuhiro Matsumoto, Masamitsu Nakazato

Chapter Twenty-Six - Clinical Trial of Ghrelin Synthesis Administration for Upper GI Surgery
Pages 409-431
Shuji Takiguchi, Yuichiro Hiura, Yasuhiro Miyazaki, Akihiro Takata, Kohei Murakami, Yuichiro Doki

Author Index
Pages 433-440

Subject Index
Pages 441-452