Genetic Engineering: Principles and Methods Volume 9

Front Matter....Pages N2-viii
Gene Transfer in the Sea Urchin....Pages 1-25
Properties and Uses of Heat Shock Promoters....Pages 27-44
The Expression of Introduced Genes in Regenerated Plants....Pages 45-59
Control of Maize Zein Gene Expression....Pages 61-74
DNase I Footprinting as an Assay for Mammalian Gene Regulatory Proteins....Pages 75-87
Use of Gene Transfer in the Isolation of Cell Surface Receptor Genes....Pages 89-103
A New Method for Synthesizing RNA on Silica Supports....Pages 105-120
Activity Gels: Reformation of Functional Proteins in SDS-Polyacrylamide Gels....Pages 121-133
Plasmid Vectors Carrying the Replication Origin of Filamentous Single-Stranded Phages....Pages 135-154
High Level Production of Proteins in Mammalian Cells....Pages 155-198
Plant Microinjection Techniques....Pages 199-227
Genetic Transformation to Confer Resistance to Plant Virus Disease....Pages 229-247
Alternative Splicing: Mechanistic and Biological Implications of Generating Multiple Proteins from a Single Gene....Pages 249-280
Back Matter....Pages 281-292