Genetic Engineering: Principles and Methods

Front Matter....Pages i-xvi
Cloning and Characterization of DNAs with Palindromic Sequences....Pages 1-11
DNA Isolation, Manipulation and Characterization from Old Tissues....Pages 13-32
Growth Factors and Neural Connectivity....Pages 33-47
Gene Identification by 3′ Terminal Exon Trapping....Pages 49-56
Engineering Transgenes for USE in the Mammary Gland....Pages 57-81
Problems That Can Limit the Expression of Foreign Genes in Plants: Lessons to be Learned From B.t. Toxin Genes....Pages 83-99
Renaturation and Reconstitution of Functional Holoenzyme From Recombinant Subunits of Casein Kinase II Expressed as Inclusion Bodies in E. Coli ....Pages 101-110
Plant Acyl-ACP Thioesterases: Chain-Length Determining Enzymes in Plant Fatty Acid Biosynthesis....Pages 111-133
Genetic Engineering of an Insect Parasite....Pages 135-155
The Stop Signal Controls the Efficiency of Release Factor-Mediated Translational Termination....Pages 157-182
Mechanism of Replication and Copy Number Control of Plasmids in Gram-Positive Bacteria....Pages 183-201
Pathways of Protein Remodeling by Escherichia Coli Molecular Chaperones....Pages 203-217
Pheromones and Pheromone Receptors as Mating-Type Determinants in Basidiomycetes....Pages 219-247
Chemical Synthesis and Applications of Phosphopeptides....Pages 249-278
Back Matter....Pages 279-289