Gene amplification in mammalian cells: a comprehensive guide

Cover......Page 1
Half Title......Page 2
Title Page......Page 4
Copyright Page......Page 5
Foreword......Page 6
Preface......Page 16
Table of Contents......Page 18
Contributors......Page 24
Introduction to Gene Amplification: Historical Background and Biological Perspective......Page 34
Part I: Organization and Structure of Amplified Genes......Page 60
 1. Cytogenetic Consequences of Gene Amplification: HSRs and DMs in Antifolate-Resistant and Multidrug-Resistant Chinese Hamster and Mouse Cells and in Human Neuroblastoma Cells with Amplified N-myc Proto- Oncogenes......Page 62
 2. Organization and Structure of Dihydrofolate Reductase Amplicons......Page 76
 3. Analysis of Gene Amplification by In-Gel DNA Renaturation......Page 88
 4. Molecular Cytogenetic Characterization of the Sequential Development of Multidrug Resistance in Two Panels of Colchicine-Resistant Cell Lines......Page 104
 5. Effects of Methotrexate on Recombinant Sequences in Mammalian Cells......Page 118
Part II: Gene Amplification and Drug Resistance......Page 128
 6. Threonyl-tRNA Synthetase Gene Amplification in Borrelidin-Resistant Mammalian Cells......Page 130
 7. Amplification of Na,K-ATPase in Ouabain-Resistant HeLa Variants......Page 140
 8. Asparagine Synthetase Gene Amplification in Albizziin-Resistant Hamster and Human Cell Lines......Page 152
 9. Overproduction of Damage Recognition Proteins in Human Cells Resistant to Cisplatin and Ultraviolet Radiation......Page 164
 10. Ornithine Decarboxylase Gene Amplification......Page 174
 11. Amplification of the IMP Dehydrogenase Gene in Mammalian Cells......Page 182
 12. Amplification and Analysis of the Mouse Dihydrofolate Reductase Gene......Page 192
 13. Amplification of the Adenylate-Deaminase 2 and Linked Genes in Chinese Hamster Lung Fibroblasts......Page 206
 14. Organization and Amplification of Metallothionein Genes in Eukaryotic Cells......Page 218
 15. Thymidylate Synthetase Gene Amplification in Drug-Resistant Mammalian Cells......Page 228
 16. Adenosine Deaminase: A Dominant Amplifiable Genetic Marker......Page 240
 17. Amplification of the HPRT Gene......Page 256
 18. Amplification of the Gene for N-Acetylglucosaminyl Phosphate Transferase, an Enzyme Catalyzing an Early Step in Asn-Linked Glycosylation, in Chinese Hamster Ovary Cells Resistant to Tunicamycin......Page 266
 19. Amplification of CAD Genes......Page 276
 20. A Reversible Selection System for UMP Synthase Gene Amplification and Deamplification......Page 288
 21. Amplification of the Genes Encoding the R1 and R2 Subunits of Mammalian Ribonucleotide Reductase......Page 298
 22. DNA Amplification in Histidinol-Resistant Chinese Hamster Ovary Cells......Page 308
 23. Amplification of the P-Glycoprotein Gene Family and Multidrug Resistance......Page 320
 24. Glutamine Synthetase Gene Amplification in Chinese Hamster Ovary Cells......Page 334
Part III: Amplification of Transferred Genes......Page 346
 25. Amplification and Expression of Transfected Genes in Mammalian Cells......Page 348
 26. Consequences of Coamplification of Adenosine Deaminase and Nonselected Genes in a Heterodiploid Host......Page 378
 27. Production of Proteins by Mammalian Cells Using Dominant Selectable and Amplifiable Markers......Page 388
Part IV: Gene Amplification and Cancer......Page 402
 28. Oncogene Amplification: Analysis of myc Oncoproteins......Page 404
 29. Biological and Clinical Significance of MYCN Amplification in Human Neuroblastomas......Page 416
 30. Amplification of the c-abl Oncogene in Chronic Myelogenous Leukemia......Page 430
 31. Gene Amplification in Small Cell Carcinoma of the Lung......Page 438
 32. Amplification and Mutagenesis of the Acetylcholinesterase and Butyrylcholinesterase Genes in Primary Human Tumors......Page 450
 33. Gene Amplification in Nontumorigenic and Tumorigenic Mammalian Cells: Relationship to Carcinogenesis......Page 462
Part V: The Mechanism of Gene Amplification......Page 472
 34. Mechanisms of Arsenic-Induced Gene Amplification......Page 474
 35. Involvement of Inverted Duplications in the Generation of Gene Amplification in Mammalian Cells......Page 480
 36. The Genetics of Amplification......Page 494
 37. \"U-Turn\" Replication at Arrested Forks Leads to the Generation of Extrachromosomal Inverted Repeats......Page 504
 38. The Stimulation of Gene Amplification in Mammalian Cells by HSAG Middle Repetitive Elements......Page 518
 39. Amplisomes: Novel Submicroscopic Extrachromosomal Elements Containing Amplified Genes......Page 532
 40. Cytogenetic and Molecular Dynamics of Mammalian Gene Amplification: Evidence Supporting Chromosome Breakage as an Initiating Event......Page 546
 A......Page 566
 C......Page 567
 D......Page 569
 G......Page 570
 I......Page 571
 M......Page 572
 O......Page 573
 R......Page 574
 T......Page 575
 Y......Page 576