G Proteins

Content: 
Contributors
Pages ix-xiii

Preface
Page xv+(1*)
Patrick C. Roche

Methods in neurosciences
Pages xvii-xviii

[1] G proteins controlling differentiation, growth, and development: Analysis by antisense RNA/DNA technology Original Research Article
Pages 1-18
Christopher M. Moxham, Hsien-yu Wang, Craig C. Malbon

[2] Analysis of adenylylcyclase subspecies gene expression in brain by in Situ hybridization histochemistry Original Research Article
Pages 19-33
Nicole Mons

[3] Site-directed mutagenesis of G protein α subunits: Analysis of αz phosphorylation and fatty acid acylation Original Research Article
Pages 34-49
Hazem Hallak, Karen M. Lounsbury, David R. Manning

[4] G protein activity in solubilized membrane preparations Original Research Article
Pages 50-57
Irene Litosch, Hong Xie

[5] Cross-linking of synaptoneurosome G proteins Original Research Article
Pages 58-63
Sherry Coulter, Saleem Jahangeer, Martin Rodbell

[6] Quantitative reconstitution and ADP-ribosylation assays for G proteins Original Research Article
Pages 64-74
Joel Abramowitz

[7] Functional assays to study coupling of dopamine D2 receptors to G proteins Original Research Article
Pages 75-89
Ja-Hyun Baik, Madhav Bhatia, Roberto Picetti, Graziella Thiriet, Emiliana Borrelli

[8] Assays for G protein βγ subunit activity Original Research Article
Pages 90-100
Joyce B. Higgins, Patrick J. Casey

[9] α2-adrenergic receptor coupling to G proteins Original Research Article
Pages 101-119
Ann E. Remmers

[10] Somatostatin receptor coupling to G proteins Original Research Article
Pages 120-132
John R. Hadcock, Joann Strnad

[11] Galanin receptor-G protein interactions and stimulation of GTPase activity Original Research Article
Pages 133-142
Susan L. Gillison, Gunnar Skoglund, Geoffrey W.G. Sharp

[12] Cloning and site-directed mutagenesis studies of gonadotropin-releasing hormone receptor Original Research Article
Pages 143-169
Stuart C. Sealfon, Wei Zhou, Niva Almaula, Vladimir Rodic

[13] Use of Xenopus laevis expression system for structure-function analysis of G-protein-coupled receptor for parathyroid hormone and parathyroid hormone-related protein Original Research Article
Pages 170-186
Paul R. Turner, Zhengmin Huang, Robert A. Nissenson, Dolores M. Shoback

[14] Production of G protein α-subunit-specific monoclonal antibodies Original Research Article
Pages 187-206
Xiaohua Li, Richard S. Jope

[15] Expression and purification of G protein βγ subunits using baculovirus expression system Original Research Article
Pages 207-226
Stephen G. Graber, Margaret A. Lindorfer, James C. Garrison

[16] Analysis of G protein γ subunits using baculovirus expression system: Requirement for posttranslational processing Original Research Article
Pages 227-245
Janet D. Robishaw

[17] Expression, purification, and functional reconstitution of recombinant phospholipase C-β isozymes Original Research Article
Pages 246-263
Andrew Paterson, T. Kendall Harden

[18] Measuring cooperative binding between retinal rod G protein, transducin, and activated rhodopsin Original Research Article
Pages 264-279
Barry M. Willardson, Tatsuro Yoshida, Mark W. Bitensky

[19] Crosstalk between tyrosine kinase and G-protein-linked signal transduction systems Original Research Article
Pages 280-297
Ross D. Feldman, Michel Bouvier

[20] Subcellular distribution and posttranslational modifications of GTP-binding proteins in insulin-secreting cells Original Research Article
Pages 298-318
Anjaneyulu Kowluru, Stewart A. Metz

[21] Epidermal growth factor-mediated regulation of G proteins and adenylylcyclase in cardiac muscle Original Research Article
Pages 319-343
Tarun B. Patel, Hui Sun, Helen Poppleton, Bipin G. Nair, Hani M. Rashed, Yi-Ming Yu

[22] G protein dependence of α1-adrenergic receptor subtype action in cardiac myocytes Original Research Article
Pages 344-361
Vitalyi Rybin, Hyung-Mee Han, Susan F. Steinberg

[23] Gsα mutations and pituitary adenomas Original Research Article
Pages 362-378
Joseph M. Alexander

[24] Molecular methods for analysis of genetic polymorphisms: Application to the molecular genetic study of genes encoding β2-adrenoceptor and stimulatory G protein α subunit Original Research Article
Pages 379-400
Charles W. Emala, Michael A. Levine

Index
Pages 401-409