G Protein Pathways Part C: Effector Mechanisms

Content: 
Contributors to volume 345
Pages xi-xvi

Preface
Pages xvii-xix
John D. Hildebrandt, Ravi Iyengar

Volume in series
Pages xxi-xl

Use of kinetikit and GENESIS for modeling signaling pathways Original Research Article
Pages 3-23
Upinder S. Bhalla

Assays of G protein/cGMP-phosphodiesterase interactions Original Research Article
Pages 27-37
Alexey E. Granovsky, Nikolai O. Artemyev

Assaying G protein-phosphodiesterase interactions in sensory systems Original Research Article
Pages 37-48
Kosei Moriyama, Manjiri M. Bakre, Farooq Ahmed, Nancy Spickofsky, Marianna Max, Robert F. Margolskee

Studies of endogenous G-protein-mediated pathways in neurons by whole-cell electrophysiology Original Research Article
Pages 51-59
Arun Anantharam, MarГ­a A. DiversГ©-Pierluissi

Biochemical approaches to study interaction of calcium channels with RGS12 in primary neuronal cultures Original Research Article
Pages 60-70
Arun Anantharam, MarГ­a A. DiversГ©-Pierluissi

Assaying phosphatidylinositol bisphosphate regulation of potassium channels Original Research Article
Pages 71-92
Tibor RohГЎcs, Coeli Lopes, Tooraj Mirshahi, Taihao Jin, Hailin Zhang, Diomedes E. Logothetis

Purification of soluble adenylyl cyclase Original Research Article
Pages 95-105
Jochen Buck Meeghan, L. Sinclair, Lonny R. Levin

Calcium-sensitive adenylyl cyclase/aequorin chimeras as sensitive probes for discrete modes of elevation of cytosolic calcium Original Research Article
Pages 105-112
Dermot M.F. Cooper

Kinetic analysis of the action of P-site analogs Original Research Article
Pages 112-126
Carmen W. Dessauer

Expression, purification, and assay of cytosolic (catalytic) domains of membrane-bound mammalian adenylyl cyclases Original Research Article
Pages 127-140
Mark E. Hatley, Alfred G. Gilman, Roger K. Sunahara

Identification of putative direct effectors for GО±o, using yeast two-hybrid method Original Research Article
Pages 140-149
J. Dedrick Jordan, Ravi Iyengar

Identification of transmembrane adenylyl cyclase isoforms Original Research Article
Pages 150-159
Martin J. Cann, Lonny R. Levin

Functional analyses of type V adenylyl cyclase Original Research Article
Pages 160-187
Tarun B. Patel, Claus Wittpoth, Ann J. Barbier, Yinges Yigzaw, Klaus Scholich

Photoaffinity labeling of adenylyl cyclase Original Research Article
Pages 188-197
Michael K. Sievert, GГјlhan Pilli, Yu Liu, Elizabeth M. Sutkowski, Kenneth B. Seamon, Arnold E. Rijoho

Crystallization of complex between soluble domains of adenylyl cyclase and activated GsО± Original Research Article
Pages 198-206
John J.G. Tesmer, Roger K. Sunahara, David A. Fancy, Alfred G. Gilman, Stephen R. Sprang

Generation of adenylyl cyclase knockout mice Original Research Article
Pages 206-231
Scott T. Wong, Daniel R. Storm

Construction of soluble adenylyl cyclase from human membrane-bound type 7 adenylyl cyclase Original Research Article
Pages 231-241
Shui-Zhong Yan, Wei-Jen Tang

Genetic selection of regulatory mutants of mammalian adenylyl cyclases Original Research Article
Pages 241-251
Peter Clapp, Austin B Capper, Ronald Taussig

Expression and characterization of rat brain phospholipase D Original Research Article
Pages 255-264
Zhi Xie, Ha Kun Kim, John H. Exton

G-protein-coupled receptor regulation of phospholipase D Original Research Article
Pages 265-274
Guangwei Du, Andrew J. Morris, Vicki A. Sciorra, Michael A. Frohman

Analysis and quantitation of ceramide Original Research Article
Pages 275-294
Paola Signorelli, Yusuf A. Hannun

Assays for phospholipase D reaction products Original Research Article
Pages 294-305
Yuhuan Xie, Kathryn E. Meier

Determination of strength and specificity of membrane-bound G protein-phospholipase C association using fluorescence spectroscopy Original Research Article
Pages 306-327
Suzanne Scarlata

Assays and characterization of mammalian phosphatidylinositol 4, 5-bisphosphate-sensitive phospholipase D Original Research Article
Pages 328-334
Xuejun Jiang, Stephen Gutowski, William D. Singer, Paul C. Sternweis

Characterization and purification of phosphatidylinositol trisphosphate 5-phosphatase from rat brain tissues Original Research Article
Pages 335-345
Rudiger Woscholski

Assay of Cdc42, Rac, and Rho GTPase activation by affinity methods Original Research Article
Pages 349-359
Valerie Benard, Gary M. Bokoch

Assays of ADP-ribosylation factor function Original Research Article
Pages 359-370
Jun Kuai, Richard A. Kahn

Functional characterization of p115 RhoGEF Original Research Article
Pages 371-382
Clark Wells, Xuejun Jiang, Stephen Gutowski, Paul C. Sternweis

Nonisotopic methods for detecting activation of small G proteins Original Research Article
Pages 383-397
Kendall D. Carey, Philip J.S. Stork

BIG1 and BIG2: Brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors Original Research Article
Pages 397-404
Gustavo Pacheco-Rodriguez, Joel Moss, Martha Vaughan

Functional interaction of GО±13 with p115RhoGEF determined with transcriptional reporter system Original Research Article
Pages 404-410
Junhao Mao, Dianqing Wu

Analysis of c-Jun n-terminal kinase regulation and function Original Research Article
Pages 413-425
J. Perry Hall, Roger J. Davis

Double-label confocal microscopy of phosphorylated protein kinases involved in long-term potentiation Original Research Article
Pages 426-436
Maria Grazia Giovannini

Regulation of mitogen-activated protein kinases by G-protein-coupled receptors Original Research Article
Pages 437-447
Mario Chiariello, J. Silvio Gutkind

Analysis of protein kinase B/Akt Original Research Article
Pages 448-463
Michelle M. Hill, Brian A. Hemmings

Direct stimulation of Bruton's tyrosine kinase by G protein a subunits Original Research Article
Pages 464-469
William E. Lowry, Yong-Chao Ma, Svetlana Cvejic, Xin-Yun Huang

Isozyme-specific inhibitors and activators of protein kinase C Original Research Article
Pages 470-489
Deborah Schechtman, Daria Mochly-Rosen

Assay of Raf-1 activity Original Research Article
Pages 490-498
Jürgen Möller, Deborah K. Morrison

Analyzing protein kinase C activation Original Research Article
Pages 499-506
Alexandra C. Newton

Assays for protein-tyrosine phosphatases Original Research Article
Pages 507-518
Daniel F. McCain, Zhong-Yin Zhang

Differential display of mRNAs regulated by G-protein signaling Original Research Article
Pages 521-529
Hironori Edamatsu, Yoshito Kaziro, Hiroshi Itoh

Gene profiling of transgenic mice with targeted expression of activated heterotrimeric G protein О± subunits using DNA microarray Original Research Article
Pages 529-539
Hsien-Yu Wang, Xiaosong Song, Xi-Ping Wang, Jiangchuan Tao

Retroviral vectors applied to gene regulation studies Original Research Article
Pages 539-551
T.J. Murphy, Grace K. Pavlath, Xiaofei Wang, Valerie Boss, Karen L. Abbott, Aaron M. Robida, Jim Nichols, Kaiming Xu, Michelle L. Ellington, James R. Loss II

Overexpression of tightly regulated proteins: protein phosphatase 2A overexpression in NIH 343 cells Original Research Article
Pages 551-555
Prahlad T. Ram

Monitoring G-protein-coupled receptor signaling with DNA microarrays and real-time polymerase chain reaction Original Research Article
Pages 556-569
Tony Yuen, Wen Zhang, Barbara J. Ebersole, Stuart C. Sealfon

cAMP response element-mediated gene expression in transgenic reporter gene mouse strain Original Research Article
Pages 570-584
Karl Obrietan, Soren Impey, Daniel R. Storm

Functional genomic search of G-protein-coupled receptors using microarrays with normalized cDNA library Original Research Article
Pages 585-600
Susumu Katsuma, Satoshi Shiojima, Akira Hirasawa, Yasuhito Suzuki, Hiroshi Ikawa, Kazuchika Takagaki, Yoshinori Kaminishi, Masatoshi Murai, Tadaaki Ohgi, Junichi Yang, Gozoh Tsujimoto

Author index
Pages 601-633

Subject index
Pages 635-653