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ویرایش: 1
نویسندگان: Ravi Iyengar. John D. Hildebrandt (Eds.)
سری: Methods in Enzymology 344
ISBN (شابک) : 9780121822453
ناشر: Academic Press
سال نشر: 2002
تعداد صفحات: 850
زبان: English
فرمت فایل : PDF (درصورت درخواست کاربر به PDF، EPUB یا AZW3 تبدیل می شود)
حجم فایل: 21 مگابایت
در صورت تبدیل فایل کتاب G Protein Pathways, Part B: G Proteins and their Regulators به فرمت های PDF، EPUB، AZW3، MOBI و یا DJVU می توانید به پشتیبان اطلاع دهید تا فایل مورد نظر را تبدیل نمایند.
توجه داشته باشید کتاب پروتئین G، بخش B: پروتئین G و تنظیم کننده های آنها نسخه زبان اصلی می باشد و کتاب ترجمه شده به فارسی نمی باشد. وبسایت اینترنشنال لایبرری ارائه دهنده کتاب های زبان اصلی می باشد و هیچ گونه کتاب ترجمه شده یا نوشته شده به فارسی را ارائه نمی دهد.
این جلد موضوعاتی مانند ساختار و شناسایی حوزههای عملکردی
پروتئینهای G و فعالسازی پروتئینهای G توسط گیرندهها یا
تنظیمکنندههای دیگر را پوشش میدهد. این متن رویکردی یکپارچه
برای مطالعه سوالات تجربی رایج در سطوح مختلف مرتبط با پروتئین
های G دارد. روشهای مربوط به پروتئینهای G با استفاده از
مدلسازی مولکولی، زیستشناسی سیستمها، مهندسی پروتئین، بیوشیمی
پروتئین، زیستشناسی سلولی و فیزیولوژی همگی در یک حجم قابل
دسترسی هستند.
استاندارد آزمایشگاهی تحسین شده برای بیش از چهل سال، روش ها
در آنزیمولوژی یکی از معتبرترین انتشارات در زمینه بیوشیمی
است. از سال 1955، هر جلد مشتاقانه مورد انتظار، مکرراً مورد
مشورت و تمجید محققان و داوران قرار گرفته است. اکنون با بیش از
300 جلد (همه آنها هنوز در دست چاپ هستند)، این مجموعه حاوی مطالب
زیادی است که امروزه هنوز مرتبط است - واقعاً یک نشریه ضروری برای
محققان در همه زمینه های علوم زیستی.
This volume covers topics such as the structure and
identification of functional domains of G proteins, and
activation of G proteins by receptors or other regulators. The
text takes an integrated approach to studying common
experimental questions at many different levels related to G
proteins. Methods related to G proteins using molecular
modeling, systems biology, protein engineering, protein
biochemistry, cell biology, and physiology are all accessible
in the same volume.
The critically acclaimed laboratory standard for more than
forty years, Methods in Enzymology is one of the most
highly respected publications in the field of biochemistry.
Since 1955, each volume has been eagerly awaited, frequently
consulted, and praised by researchers and reviewers alike. Now
with more than 300 volumes (all of them still in print), the
series contains much material still relevant today—truly an
essential publication for researchers in all fields of life
sciences
Content:
Contributors to volume 344
Pages xi-xvi
Preface
Pages xvii-xix
John D. Hildebrandt, Ravi Iyengar
Volume in series
Pages xxi-xl
Analysis of G protein activation in Sf9 and mammalian cells by agonist-promoted [35S] GTОіS Binding Original Research Article
Pages 3-14
Rolf T. Windh, David R. Manning
Elucidating kinetic and thermodynamic constants for interaction of g protein subunits and receptors by surface plasmon resonance spectroscopy Original Research Article
Pages 15-42
R. Victor Rebois, Peter Schuck, John K. Northup
Neuroanatomical localization of receptor-activated G proteins in brain Original Research Article
Pages 42-58
Laura J. Sim-Selley, Steven R. Childers
Design and use of C-terminal minigene vectors for studying role of heterotrimeric G proteins Original Research Article
Pages 58-69
Annette Gilchrist, Anli Li, Heidi E. Hamm
Dissecting receptor-G protein specificity using GО± chimeras Original Research Article
Pages 69-81
Theresa M. Cabrera-Vera, Tarita O. Thomas, Jurgen Vanhauwe, Karyn M. Depree, Stephen G. Graber, Heidi E. Hamm
Use of dominant negative mutations in analysis of G protein function in Saccharomyces cervisiae Original Research Article
Pages 82-91
Gregor Jansen, Ekkehard Leberer, David Y. Thomas, Malcolm Whiteway
Functional assays for mammalian G-protein-coupled receptors in yeast Original Research Article
Pages 92-111
Pamela E. Mentesana, Mercedes Dosil, James B. Konopka
Role of G protein ОІОі complex in receptor-G protein interaction Original Research Article
Pages 112-125
Inaki Azpiazu, N. Gautam
Phosducin down-regulation of G-protein coupling: Reconstitution of phosducin and transducin of cGMP cascade in bovine rod photoreceptor cells Original Research Article
Pages 126-139
Yee-Kin Ho, Tuow Daniel Ting, Rehwa Ho Lee
Analysis of signal transfer from receptor to in different membrane environments and receptor-independent activators of brain G protein Original Research Article
Pages 140-152
Catalina Ribas, Motohiko Sato, John D. Hildebrandt, Stephen M. Lanier
Identification of modulators of mammalian G-protein signaling by functional screens in the yeast Saccharomyces cerevisiae Original Research Article
Pages 153-168
Mary J. Cismowski, Aya Takesono, Chienling Ma, Stephen M. Lanier, Emir Duzic
Expression of О± subunit of GS in Escherichia coli Original Research Article
Pages 171-175
Shui-Zhong Yan, Wei-Jen Tang
Purification of G protein isoforms GOA and GOC from bovine brain Original Research Article
Pages 176-185
Jane Dingus, William E. McIntire, Michael D. Wilcox, John D. Hildebrandt
Coexpression of proteins with methionine aminopeptidase and/or N-myristoyltransferase in Escherichia coli to Increase acylation and homogeneity of protein preparations Original Research Article
Pages 186-193
Hillary A. Van Valkenburgh, Richard A. Kahn
Purification of G protein ОІОі from bovine brain Original Research Article
Pages 194-208
Jane Dingus, Bronwyn S. Tatum, Govindan Vaidyanathan, John D. Hildebrandt
Separation and analysis of G protein Оі subunits Original Research Article
Pages 209-233
Lana A. Cook, Michael D. Wilcox, Jane Dingus, Kevin L. Schey, John D. Hildebrandt
Activity of GОі prenylcysteine carboxyl methyltransferase Original Research Article
Pages 234-245
David Michaelson, Mark R. Philips
Preparation and application of G protein Оі subunit-derived peptides incorporating a photoactive isoprenoid Original Research Article
Pages 245-258
Tamara A. Kale, Tammy C. Turek, Vanessa Chang, N. Gautam, Mark D. Distefano
Expression and functional analysis of G protein О± subunits in S49 lymphoma cells Original Research Article
Pages 261-277
Catherine H. Berlot
Mouse gene knockout and knockin strategies in application to О± subunits of Gi/Go family of G proteins Original Research Article
Pages 277-298
Meisheng Jiang, Karsten Spicher, Guylain Boulay, Angeles MartГn-Requero, Catherine A. Dye, Uwe Rudolph, Lutz Birnbaumer
Determining cellular role of GО±12 Original Research Article
Pages 298-309
Jonathan M. Dermott, N. Dhanasekaran
Targeted, regulatable expression of activated heterotrimeric G protein О± subunits in transgenic mice Original Research Article
Pages 309-318
Xiaosong Song, Jiangchuan Tao, Xi-Ping Huang, Thomas A. Rosenquist, Craig C. Malbon, Hsien-Yu Wang
Inducible, tissue-specific suppression of heterotrimeric G protein О± subunits in vivo Original Research Article
Pages 318-327
Xi-Ping Huang, Thomas A. Rosenquist, Hsien-Yu Wang, Craig C. Malbon
Construction of replication defective adenovirus that expresses mutant GО±sQ227L Original Research Article
Pages 328-343
Tara Ann Santore, Ravi Iyengar
Expression of adenovirus-directed expression of activated GПѓs in rat hippocampal slices Original Research Article
Pages 343-349
George P. Brown, Ravi Iyengar
Quech-flow kinetic measurement of individual reactions of G-protein-catalyzed GTPase cycle Original Research Article
Pages 350-369
Suchetana Mukhopadhyay, Elliott M. Ross
Analysis of genomic imprinting of GsО± gene Original Research Article
Pages 369-383
Lee S. Weinstein, Shuhua Yu, Jie Liu
Subcellular localization of G protein subunits Original Research Article
Pages 383-403
Susanne M. Mumby
Fluorescence approaches to study G protein mechanisms Original Research Article
Pages 403-420
Dyke P. Ewen, Kyle R. Gee, Hee C. Kang, Richard R. Neubig
Defining G protein ОІОі specificity for effector recognition Original Research Article
Pages 421-434
E.J. Dell, Trillium Blackmer, Nikolai P. Skiba, Yehia Daaka, Lee R. Shekter, Ramon Rosal, Eitan Reuveny, Heidi E. Hamm
Ribozyme-mediated suppression of G protein Оі subunits Original Research Article
Pages 435-451
Janet D. Robishaw, Qin Wang, William F. Schwindinger
Use of scanning mutagenesis to delineate structure-function relationships in G protein О± subunits Original Research Article
Pages 455-468
Catherine H. Berlot
Development of Gs-selective inhibitory compounds Original Research Article
Pages 469-480
Christian Nanoff, Oliver Kudlacek, Michael Freissmuth
Characterization of deamidated G protein subunits Original Research Article
Pages 481-505
William E. McIntire, Kevin L. Schey, Daniel R. Knapp, Jane Dingus, John D. Hildebrandt
Determining G protein heterotrimer formation Original Research Article
Pages 505-512
Yongmin Hou, Vanessa Chang, N. Gautam
Use of peptide probes to determine function of interaction sites in G protein interactions with effectors Original Research Article
Pages 513-521
Elizabeth Buck, Ravi Iyengar
Protein interaction assays with G proteins Original Research Article
Pages 521-535
Guangyu Wu, Michael L. Bernard, Stephen M. Lanier
Evolutionary traces of functional surfaces along G protein signaling pathway Original Research Article
Pages 536-556
Olivier Lichtarge, Mathew E. Sowa, Anne Philippi
Discovery of ligands for ОІОі subunits from phage-displayed peptide libraries Original Research Article
Pages 557-576
Alan V. Smrcka, Jamie K. Scott
Exploring protein-protein interactions by peptide docking protocols Original Research Article
Pages 577-586
Gezhi Weng
Structural characterization of intact G protein Оі subunits by mass spectrometry Original Research Article
Pages 586-597
Kevin L. Schey, Mark Busman, Lana A. Cook, Heidi E. Hamm, John D. Hildebrandt
Quantitative assays for GTPase-activating proteins Original Research Article
Pages 601-617
Elliott M. Ross
Analysis of RGS proteins in saccharomyces cervisiae Original Research Article
Pages 617-631
Ginger A. Hoffman, Tiffany Runyan Garrison, Henrik G. Dohlman
Purification of RGS Protein, Sst2, from Saccharomyces cereuisiae and Escherichia coli Original Research Article
Pages 632-647
Tiffany Runyan Garrison, Donald M. Apanovitch, Henrik G. Dohlman
RGS domain: Production and uses of recombinant protein Original Research Article
Pages 647-657
David Yowe, Kan Yu, Thomas M. Wilkie, Serguei Popov
Screening for interacting partners for GО±i3 and RGS-GRIP using the two-hybrid system Original Research Article
Pages 657-673
Luc de Vries, Marilyn Gist Farquhar
Assay of RGS protein activity in vitro using purified components Original Research Article
Pages 673-685
Andrejs M. Krumins, Alfred G. Gilman
Measuring RGS protein interactions with GqО± Original Research Article
Pages 686-702
Peter Chidiac, Martha E. Gadd, John R. Hepler
Assays of complex formation between RGS protein GОі subunit-like domains and GОІ subunits Original Research Article
Pages 702-723
David P. Siderovski, Bryan E. Snow, Stephen Chung, Greg M. Brothers, John Sondek, Laurie Betts
RGS function in visual signal transduction Original Research Article
Pages 724-740
Wei He, Theodore G. Wensel
Molecular cloning of regulators of G-protein signaling family members and characterization of binding specificity of RGS 12 PDZ domain Original Research Article
Pages 740-761
Bryan E. Snow, Greg M. Brothers, David P. Siderovski
Author index
Pages 763-792
Subject index
Pages 793-813