Functional Organization of the Nucleus: A Laboratory Guide

Content: 
Series Editor
Page ii

Editorial Page
Page iii

Copyright Page
Page iv

Contributors
Pages xiii-xv

Preface
Pages xvii-xviii

Chapter 1 DNA Sequence Localization in Metaphase and Interphase Cells by Fluorescence in Situ Hybridization Original Research Article
Pages 3-35
Barbara J. Trask

Chapter 2 Localization of mRNAs by in Situ Hybridization Original Research Article
Pages 37-71
Lynne M. Angerer, Robert C. Angerer

Chapter 3 Fluorescent Detection of Nuclear RNA and DNA: Implications for Genome Organization Original Research Article
Pages 73-99
Carol Villnave Johnson, Robert H. Singer, Jeanne Bentley Lawrence

Chapter 4 Visualization of DNA Sequences in Meiotic Chromosomes Original Research Article
Pages 101-108
Peter B. Moens, Ronald E. Pearlman

Chapter 5 Nucleic Acid Sequence Localization by Electron Microscopic in Situ Hybridization Original Research Article
Pages 109-132
Sandya Narayanswami, Nadja Dvorkin, Barbara A. Hamkalo

Chapter 6 The Use of Autoantibodies in the Study of Nuclear and Chromosomal Organization Original Research Article
Pages 135-175
W.C. Earnshaw, J.B. Rattner

Chapter 7 Meiotic Chromosome Preparation and Protein Labeling Original Research Article
Pages 177-202
C. Heyting, A.J.J. Dietrich

Chapter 8 Distribution of Chromosomal Proteins in Polytene Chromosomes of Drosophila Original Research Article
Pages 203-227
Robert F. Clark, Cynthia R. Wagner, Carolyn A. Craig, Sarah C.R. Elgin

Chapter 9 The Use of Monoclonal Antibody Libraries Original Research Article
Pages 229-252
H. Saumweber

Chapter 10 Optical Sectioning and Three-Dimensional Reconstruction of Diploid and Polytene Nuclei Original Research Article
Pages 253-286
Mary C. Rykowski

Chapter 11 Yeast Minichromosomes Original Research Article
Pages 289-314
Sharon Y. Roth, Robert T. Simpson

Chapter 12 Nucleosomes of Transcriptionally Active Chromatin: Isolation of Template-Active Nucleosomes by Affinity Chromatography Original Research Article
Pages 315-335
Vincent G. Allfrey, Thelma A. Chen

Chapter 13 The Nucleoprotein Hybridization Method for Isolating Active and Inactive Genes as Chromatin Original Research Article
Pages 337-367
Claudius Vincenz, Jan Fronk, Graeme A. Tank, Karen Findling, Susan Klein, John P. Langmore

Chapter 14 Protein—DNA Cross-Linking as a Means to Determine the Distribution of Proteins on DNA in Vivo Original Research Article
Pages 369-381
David S. Gilmour, Ann E. Rougvie, John T. Lis

Chapter 15 Protein—DNA Interactions in Vivo—Examining Genes in Saccharomyces cerevisiae and Drosophila melanogaster by Chromatin Footprinting Original Research Article
Pages 383-415
Melissa W. Hull, Graham Thomas, Jon M. Huibregtse, David R. Engelke

Chapter 16 Control of Class II Gene Transcription during in Vitro Nucleosome Assembly Original Research Article
Pages 419-447
Jerry L. Workman, Ian C.A. Taylor, Robert E. Kingston, Robert G. Roeder

Chapter 17 Systems for the Study of Nuclear Assembly, DNA Replication, and Nuclear Breakdown in Xenopus laevis Egg Extracts Original Research Article
Pages 449-468
Carl Smythe, John W. Newport

Chapter 18 In Vitro Nuclear Protein Import Using Permeabilized Mammalian Cells Original Research Article
Pages 469-482
Stephen A. Adam, Rachel Sterne-Marr, Larry Gerace

Chapter 19 Mutations That Affect Chromosomal Proteins in Yeast Original Research Article
Pages 485-523
M. Mitchell Smith

Chapter 20 Mutations That Affect Nuclear Organization in Yeast Original Research Article
Pages 525-541
Ann O. Sperry, Barbara R. Fishel, W.T. Garrard

Chapter 21 Mutations Affecting Cell Division in Drosophila Original Research Article
Pages 543-586
Maurizio Gatti, Michael L. Goldberg

Chapter 22 Position-Effect Variegation—An Assay for Nonhistone Chromosomal Proteins and Chromatin Assembly and Modifying Factors Original Research Article
Pages 587-627
T. Grigliattia

Index
Pages 629-661

Contents of Recent Volumes
Pages 663-670