From Gene to Protein: Translation Into Biotechnology

Content: 
MIAMI WINTER SYMPOSIA—VOLUME 19, Page ii
Front Matter, Page iii
Copyright, Page iv
SPEAKERS AND DISCUSSANTS, Pages xvii-xx
PREFACE, Pages xxi-xxii, Fazal Ahmad, Julius Schultz, Eric E. Smith, William J. Whelan
MESSING ABOUT WITH ISOTOPES AND ENZYMES AND ANTIBODIES, Pages 3-24, César Milstein
MOVING GENES: PROMISES KEPT AND PENDING, Pages 27-42, Philip Leder, Philip A. Hieter, Gregory F. Hollis, Aya Leder
CELL CULTURE AND PHYSIOLOGY, Pages 45-52, Gordon H. Sato
REGULATION OF GROWTH AND DIFFERENTIATION OF MAMMALIAN CELLS BY HORMONES AND EXTRACELLULAR MATRIX, Pages 53-73, Lola C.M. Reid
MICROCARRIERS AND THE PROBLEM OF HIGH DENSITY CELL CULTURE, Pages 75-103, William G. Thilly, Debra Barngrover, James N. Thomas
CROWN GALL — NATURE'S GENETIC ENGINEER, Pages 105-125, Milton Gordon, David Garfinkel, Harry Klee, Vic Knauf, William Kwok, Conrad Lichtenstein, Joan McPherson, Alice Montoya, Eugene Nester, Patrick O'Hara, Ann Powell, Lloyd W. Ream, Robert Simpson, Brian Taylor, Frank White
MONOCLONAL ANTIBODIES: THE PRODUCTION OF TAILOR-MADE SEROLOGICAL REAGENTS, Pages 129-142, Dale E. Yelton, Pallaiah Thammana, Catherine Desaymard, Susan B. Roberts, Sau-Ping Kwan, Angela Giusti, Donald J. Zack, Roberta R. Pollock, Matthew D. Scharff
MONOCLONAL ANTIBODIES IN THE ANALYSIS OF THE MOLECULAR BASIS FOR HUMAN GENETIC DISEASES, Pages 143-164, Roger H. Kennett, Kendra B. Eager, Barbara Meyer, Virginia Braman, Suzanne Newberry, David W. Buck
HUMAN CELL SURFACE ANTIGENS STUDIED WITH MONOCLONAL ANTIBODIES, Pages 165-174, Jack L. Strominger
IMMUNOCYTOCHEMISTRY WITH MONOCLONAL ANTIBODIES: POTENTIAL APPLICATIONS IN BASIC SCIENCES AND HISTOPATHOLOGY, Pages 175-191, A. Claudio Cuello, César Milstein
HUMAN IMMUNOGLOBULIN EXPRESSION IN HYBRID CELLS, Pages 193-210, Carlo M. Croce
THE ROLE OF SYNTHETIC DNA IN THE PREPARATION OF STRUCTURAL GENES CODING FOR PROTEINS, Pages 213-234, John J. Rossi, Ryszard Kierzek, Ting Huang, Peter Walker, Keiichi Itakura
THE SYNTHESIS AND BIOCHEMICAL REACTIVITY OF BIOLOGICALLY IMPORTANT GENES AND GENE CONTROL REGIONS, Pages 235-247, Marvin H. Caruthers
SYNTHESIS OF HUMAN INTERFERONS AND ANALOGS IN HETERO1OGOUS CELLS, Pages 249-262, R. Derynck, P.W. Gray, E. Yelverton, D.W. Leung, H.M. Shepard, R.M. Lawn, A. Ullrich, R. Najarian, D. Pennica, F.E. Hagie, R.A. Hitzeman, P.J. Sherwood, A.D. Levinson, D.V. Goeddel
MAKING MUTATIONS IN VITRO AND PUTTING THEM BACK INTO YEAST, Pages 265-274, David Botstein, David Shortle, Mark Rose
EXPRESSION OF THE HUMAN GROWTH HORMONE GENE IS REGULATED IN MOUSE CELLS, Pages 275-288, Diane M. Robins, Inbok Paek, Richard Axel, Peter H. Seeburg
TRANSFER OF THE MOUSE METALLOTHIONEIN-I GENE INTO CULTURED CELLS AND INTO ANIMALS, Pages 289-305, Richard D. Palmiter, Ralph L. Brinster
CONSTRUCTION OF THREE HYBRID PROMOTERS AND THEIR PROPERTIES IN ESCHERICHIA COLI, Pages 309-327, Hermande Boer, Herbert Heyneker, Lisa Comstock, Alice Wieland, Mark Vasser, Thomas Horn
REGULATION OF HISTONE GENE EXPRESSION IN HUMAN CELLS, Pages 331-365, G.S. Stein, L. Baumbach, A. Lichtler, F. Marashi, M. Plumb, R. Rickles, F. Sierra, T. Van Dyke, J.L. Stein, A. Leza
CLINICAL INVESTIGATION OF PARTIALLY PURE AND RECOMBINANT DNA-DERIVED LEUKOCYTE INTERFERON IN HUMAN CANCER, Pages 367-390, Jordan Gutterman, Jorge Quesada
THE USE OF BIOSYNTHETIC HUMAN INSULIN IN MAN, Pages 391-417, John A. Galloway, Mary A. Root
THE BIOLOGICAL EFFECTIVENESS OF PITUITARY-DERIVED AND BIOSYNTHETIC METHIONYL-hGH IN ANIMALS AND MAN, Pages 419-428, S.L. Kaplan, R. Hintz, R. Swift, J. Fenno, N. Stebbing
BIOLOGICAL ACTIVITY OF A CLONED HUMAN ENZYME UROKINASE, Pages 429-444, P.P. Hung
ACTIVITY OF CLONED GENE PRODUCTS IN ANIMAL SYSTEMS, Pages 445-458, Nowell Stebbing, Sang He Lee, Benedict J. Marafino, Phillip K. Weck, Kenneth W. Renton
STUDIES ON POLIOVIRUS PRODUCED BY TRANSFECTION WITH CLONED VIRAL cDNA, Pages 459-470, Vincent R. Racaniello, David Baltimore
Friday Afternoon Panel Discussion, Pages 473-503
GENES FOR CYTOCHROME P-450 AND THEIR REGULATION, Page 507, Michael Atchison, Eugene Ryvkin, Andrea Lippman, Cynthia Raphael, Milton Adesnik
LOCALIZATION OF A HEPARIN BINDING SITE OF FIBRONECTIN WITH THE AID OF MONOCLONAL ANTIBODIES, Page 508, Blair T. Atherton, Elizabeth V. Hayes, Richard O. Hynes
NUCLEOTIDE SEQUENCE OF THE glg C GENE CODING FOR ADP-GLUCOSE SYNTHETASE FROM E. COLI, Page 509, Preston A. Baecker, Clement E. Furlong, Jack Preiss
ISOLATION AND CHARACTERIZATION OF A SOYBEAN GENE FOR THE SMALL SUBUNIT OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE, Page 510, S.L. Berry, D.M. Shah, R.B. Meagher
STUDYING POLYOMA VIRUS GENE EXPRESSION AND FUNCTION BY SITEDIRECTED MUTAGENESIS USING SYNTHETIC OLIGONUCLEOTIDES, Page 511, Gordon G. Carmichael, David Dorsky, Brian Schaffhausen, Donald Oliver, Thomas L. Benjamin
CHARACTERIZATION OF FOUR DIVERGENT HUMAN GENOMIC CLONES HOMOLOGOUS TO THE TRANSFORMING P21 GENES OF HARVEY AND KIRSTEN MURINE SARCOMA VIRUSES, Page 512, Esther H. Chang, Matthew A. Gonda, Ronald W. Ellis, Edward M. Scolnick, Douglas R. Lowy
IN VITRO SYNTHESIS OF ENZYMES INVOLVED IN MEMBRANE BIOSYNTHESIS IN E. COLI, Page 513, Yang Chang Chen, William Dowhan
STUDIES ON EXPRESSION OF THE PHASEOLIN GENE OF FRENCH BEAN SEEDS IN SUNFLOWER PLANT CELLS, Page 514, Prabhakara V. Choudary, Timothy C. Hall, John D. Kemp, Dennis W. Sutton
DNA STIMULATES ATP-DEPENDENT PROTEOLYSIS AND PROTEIN-PENDENT ATPase ACTIVITY OF PROTEASE La FROM ESCHERICHIA COLI, Page 515, Chin Ha Chung, Lloyd Waxman, Alfred L. Goldberg
METHYLATION IN CELLULAR MEMBRANES OF FRIEND VIRUS TUMORS OF MICE, Page 516, W.E. Cornatzer, Dennis R. Hoffman, Judy A. Haning
IDENTIFICATION OF A NOVEL, H-2-LIKE GENE BY ANALYSIS OF cDNA CLONES, Page 517, David Cosman, George Khoury, Gilbert Jay
A GENETIC ANALYSIS OF THE GLUCOCORTICOID RESPONSE, Page 518, Mark Danielsen, Michael R. Stallcup
NUCLEOTIDE SEQUENCE OF THE REGION SURROUNDING THE 5′ END OF 18S rRNA FROM SOYBEAN INFERRED FROM THE GENE SEQUENCE, Page 519, V.K. Eckenrode, R.T. Nagao, R.B. Meagher
AN ELISA ASSAY FOR DETECTING CELL SURFACE ANTIGENS ON ADHERENT CELLS, Page 520, C Feit, A H Bartal, G Tauber, Y Hirshaut
THE INVOLVEMENT OF DNA POLYMERASE α IN ADENOVIRUS DNA REPLICATION, Page 521, David A. Foster, Geoffrey Zubay
DEVELOPMENT OF HUMAN GROWTH HORMONE PRODUCED IN RECOMBINANT BACTERIA AS A THERAPEUTIC AGENT, Page 522, L. Fryklund, K. Fhölenhag, H. Flodh, B. Holmström, A. Skottner-Lundin, B. Strindberg, A. Wichman
Expression of the Genes for the Pst Restriction-Modification Enzymes after Cloning into a Temperature-Sensitive Replication Plasmid, Page 523, Roy Fuchs, James F. Kane
THE NUCLEOTIDE SEQUENCE OF pACYC 177, Page 524, J. Galen, D. Kuebbing, L. Runkel, K. Rushlow
THE ISOLATION OF SEA NETTLE LETHAL FACTOR I VIA IMMOBILIZED MONOCLONAL ANTIBODIES, Page 525, Pramod K. Gaur, Carrington S. Cobb, A.J. Russo, Gary J. Calton, Joseph W. Burnett
SYNTHESIS OF A SPECIFIC SEQUENCE OF DNA USING RESTRICTION ENZYMES, Page 526, Richard Gayle, George Bennett
INDUCTION OF STRESS PROTEINS BY HEAT SHOCK AND METAL IONS, Page 527, L. Gedamu, N. Shworak, J.J. Heikkila, G.A. Schultz
Analysis of the Rous Sarcoma Virus Promoter Region by Site-Directed Mutagenesis, Page 528, Gregory M. Gilmartin, J.T. Parsons
CLONING AND SEQUENCING OF AN INSULIN-LIKE RNA FROM HUMAN BRAIN, Page 529, Antonio Gonzalez, Lydia Villa-Komaroff
VASOACTIVE INTESTINAL POLYPEPTIDE—FROM GENE TO PROTEIN TO PEPTIDE, Page 530, I. Gozes, R.J. Milner, R. Benoit, E. Johnson, E.L.F. Battenberg, F.E. Bloom, F.-T. Liu, R.A. Lerner, D.H. Katz
DETECTION OF SPECIFIC MOUSE HISTONE mRNAs BY SI NUCLEASE MAPPING, Page 531, Reed A. Graves, Donald B. Sittman, Ing-Ming Chiu, William F. Marzluff
ISOLATION AND CHARACTERIZATION OF HUMAN IMMUNE INTERFERON (IFN-γ) MESSENGER RNA, Page 532, Patrick W. Gray, David Leung, Pamela J. Sherwood, David V. Goeddel, Shelby L. Berger, Donald M. Wallace
MONOCLONAL ANTIBODIES THAT INHIBIT THE RAT LIVER RECEPTOR FOR ASIALOGLYCOPROTEINS, Page 533, Joe Harford, Mark Lowe, Gilbert Ashwell
SOLID PHASE NUCLEOTIDE AND PEPTIDE SYNTHESIS BY ADAPTATION OF LABORATORY HPLC EQUIPMENT, Page 534, Derek Hudson, Ronald M. Cook
CLONING AND SEQUENCING OF A cDNA FOR αA CRYSTALLIN mRNA OF THE MOUSE LENS: PRESENCE OF AN EXTENSIVE 3′ UNTRANSLATED REGION, Page 535, Charles R. King, Toshimichi Shinohara, Joram Piatigorsky
MONOCLONAL ANTIBODIES RECOGNIZING COMMON ANTIGENIC DETERMINANTS ON LYSOSOMAL ENZYMES, Page 536, David A. Knecht, Robert C. Mierendorf Jr., Randall L. Dimond
PROTEINS OF A MINIPLASMID DERIVATIVE OF THE BROAD HOST RANGE PLASMID RK2, Page 537, Jon A. Kornacki, William Firshein
MONOCLONAL IgM ANTIBODIES THAT INDUCE T CELL-MEDIATED CYTOTOXICITY OF MURINE SARCOMA CELLS, Page 538, Eddie W. Lamon, John F. Kearney
DEVELOPMENT OF METHODS FOR THE LARGE-SCALE CULTURE OF OXYTRICHA NOVA, Page 539, Tommie J. Laughlin, J. Michael Henry, Edward F. Phares, Mary V. Long, Donald E. Qlins
CLONING OF CDNA ENCODING THE SWEET-TASTING PLANT PROTEIN THAUMATIN AND ITS EXPRESSION IN ESCHERICHIA COLI, Page 540, J. Maat, L. Edens, A.M. Ledeboer, M. Toonen, C. Visser, I. Bom, C.T. Verrips
AMPLIFICATION OF THE METALLOTHIONEIN-I GENE IN CADMIUM- AND ZINC-RESISTANT CHINESE HAMSTER OVARY CELLS, Page 541, Kenneth S. McCarty Sr., Gregory G. Gick, Kenneth S. McCarty Jr.
CONSTITUTIVE THYMIDINE KINASE ACTIVITY IN DIFFERENTIATING MOUSE MYOBLASTS TRANSFORMED WITH CLONED HERPES VIRUS THYMIDINE KINASE GENES, Page 542, Gary F. Merrill, Stephen D. Hauschka
Cloning of the Gene for alpha-Amylase from Bacillus stearothermophilus into Escherichia coli, Page 543, Jonathan R. Mielenz
EXPRESSION OF FOREIGN GENES IN RECOMBINANT PLASMIDS UNDER THE CONTROL OF T7 AND T3 LATE PROMOTERS, Page 544, Claire E. Morris, William T. McAllister
THE INABILITY OF IN VITRO TRANSFORMING SV40 SUBGENOMES TO CAUSE TUMORS IN VIVO, Page 545, M.P. Moyer, R.C. Moyer
MODEL RIBOSOMES, Page 546, Tadayoshi Nakashima, Sidney W. Fox
SELECTIVE INDUCTION BY PEPTIDE GROWTH FACTORS OF THE SYNTHESIS OF SECRETED PROTEINS IN 3T3 CELLS, Page 547, Marit Nilsen-Hamilton, Richard T. Hamilton, W. Ross Allen
DELETION OF DNA SEQUENCES BETWEEN PRE-SELECTED BASES, Page 548, Nikos Panayotatos, Kim Truong
EXPRESSION OF HERPESVIRUS THYMIDINE KINASE GENE UNDER CONTROL OF EARLY PROMOTER OF SV40, Page 549, Mary M. Pater, Alan Pater, Giampiero di Mayorca, James R. Smiley
REGULATION OF HEAVY CHAIN GENE EXPRESSION IN MOUSE MYELOMA MOPC 315 CELLS, Page 550, Phyllis Ponte, Michael Dean, Vincent H. Pepe, Gail E. Sonenshein
AMPLIFICATION AND RAPID PURIFICATION OF EXONUCLEASE I FROM ESCHERICHIA COLI K-12, Page 551, Douglas Prasher, Sidney R. Kushner
ANALYSIS OF β-INTERFERON mRNA IN HUMAN CELLS, Page 552, N.B.K. Raj, Paula M. Pitha, Kevin Kelley
THE PURIFICATION OF MICROSOMAL GLUCOSE-6-PHOSPHATASE FROM HUMAN LIVER: AIDS TO THE STUDY OF GLYCOGEN STORAGE DISEASE, Page 553, Peter R. Reczek, Claude A. Villee
TRANSFER AND MAPPING OF GENES USING METAPHASE CHROMOSOMES FROM ICR 2A FROG CELLS, Page 554, Barry S. Rosenstein
INTRACELLULAR SELECTION FOR PLASMIDS THAT CARRY GENES FOR HUMAN INSULIN, Page 555, Paul R. Rosteck Jr., Charles L. Hershberger
IMMUNOELECTRON MICROSCOPIC ANALYSIS OF THE IMMUNOGLOBUL IN MOLECULE FOR IDIOTYPES AND ALLOTYPES, Page 556, Kenneth H. Roux, Dennis W. Metzger
CROSS REACTIVITY OF LETHAL VENOMS TO A MONOCLONAL ANTIBODY, Page 557, Russo A.J. Cobb, Carrington S. Calton, J. Gary, Joseph W. Burnett
CLONING OF AAV INTO pBR322: RESCUE OF INTACT VIRUS FROM THE RECOMBINANT PLASMID IN HUMAN CELLS, Page 558, Richard J. Samulski, Kenneth I. Berns, Ming Tan, Nicholas Muzyczka
GENERATION, CHARACTERIZATION, AND UTILIZATION OF MONOCLONAL ANTIBODIES REACTIVE WITH HUMAN MAMMARY CARCINOMA ASSOCIATED ANTIGENS, Page 559, J. Schlom, D. Colcher, P. Horan Hand, M. Nuti, D. Stramignoni, Y. Teramoto, D. Wunderlich
STRUCTURE OF THE ACTIN MULTIGENE FAMILY OF SOYBEAN (Glycine max), Page 560, D.M. Shah, T.D. McKnight, R.C. Hightower, B.R. Dhruva, R.B. Meagher
IDENTIFICATION OF DEFECTIVE GLOBIN mRNA IN MEL x FIBROBLAST HYBRID CELLS, Page 561, J.A. Shapiro, Y. Chiang, T. Ley, A.W. Nienhuis, W.F. Anderson
A PLASMID CLONING VECTOR FOR INDUCIBLE OVERPRODUCTION OF PROTEINS IN BACTERIAL CELLS, Page 562, A.R. Shatzman, M. Rosenberg
CHARACTERIZATION OF HUMAN GLOBIN ψβ2 — A PHANTOM PSEUDOGENE, Page 563, Shi-Hsiang Shen, Patricia A. Powers, Oliver Smithies
PRODUCTION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI, Page 564, Roger Sherwood, Jeremy Court, Ann Mothershaw, William Keevil, Derek Ellwood, George Jack, Harry Gilbert, Richard Blazek, Jim Wade, Tony Atkinson
Novel expression of an interferon gene in E, coli in high yields, Page 565, P. Slocombe, A. Easton, P. Boseley, D.C. Burke
HMG PROTEINS 1 AND 2 ARE REQUIRED FOR TRANSCRIPTION, Page 566, Jose A. Stoute, William F. Marzluff
TISSUE-SPECIFIC METHYLATION PATTERNS IN BOVINE SATELLITE DNA, Page 567, K. Sturm, R.A. McGraw, J.H. Taylor
FOXOMAS: A SECOND GENERATION METHOD FOR OBTAINING STABLE HEAVY CHAIN IMMUNOGLOBULIN PRODUCING HYBRIDOMAS, Page 568, R. Thomas Taggart
IN VITRO AND IN VIVO TRANSCRIPTIONAL ANALYSIS OF MOUSE GLOBIN GENE PROMOTERS, Page 569, Carol A. Talkington, Philip Leder
PURIFICATION OF PLASMID DNAs AND SYNTHETIC OLIGODEOXYRIBO-CLEOTIDES USING LOW PRESSURE ANION ENCHANGE CHROMATOGRAPHY, Page 570, John A. Thompson, Paul W. Armstrong, Keith E. Rushlow, Kathleen Doran, Robert W. Blakesley
MONOCLONAL ANTIBODY AGAINST OUABAIN SENSITIVITY RELATED HUMAN CELL SURFACE ANTIGEN, Page 571, Yean-Kai Tsung, Aubrey Milunsky, David Reiss
SELECTION OF MONOCLONAL ANTIBODIES FOR DESIGNATED USES, Page 572, V. van Heyningen, L. Barron, D. Brock, W.M. Hunter
TOWARD ANALYSIS OF THE HLA-DR SYSTEM WITH MONOCLONAL ANTIBODIES, Page 573, V. van Heyningen, K. Guy, B.B. Cohen, D.L. Deane, C.M. Steel
A HUMAN PROINSULIN cDNA RECOMBINANT CLONE, Page 574, L.L. Villa, R.R. Brentani
PREPARATION OF DNA LABELED WITH HIGH SPECIFIC ACTIVITY [35S] DEOXYADENOSINE 5′-(α-THIO) TRIPHOSPHATE; THE USE OF 35S-LABELED NUCLEIC ACIDS AS MOLECULAR HYBRIDIZATION PROBES, Page 575, Mark J. Vincent, William R. Beltz, Sarah H. Ashton
DNA TRANSFER OF BACTERIAL ASPARAGINE SYNTHETASE INTO MAMMALIAN CELLS, Page 576, Mary M.Y. Wave, Clifford P. Stanners
Production and Characterization of Monoclonal Antibodies to a Calcium-activated Protease, Page 577, M. Wheelock, J. Schollmeyer, W. Dayton
PHENOTYPIC EXPRESSION OF MAMMALIAN AND FOREIGN PROCARYOTIC GENES CLONED IN BACILLUS SUBTILIS, Page 578, P.M. Williams, R.G. Schoner, Y-W. Chen, P.S. Lovett
THE HUMAN α1-ANTITRYPSIN GENE AND PULMONARY EMPHYSEMA, Page 579, Savio L.C. Woo, T. Chandra, Margaret Leicht, George Long, Kotoku Kurachi, Earl W. Davie
GENE EXPRESSION DURING MAMMALIAN TOOTH ORGAN DEVELOPMENT, Page 580, Margarita Zeichner-David, Mary MacDougall, Conny Bessem, Pablo Bringas Jr., Harold C. Slavkin
INDEX OF AUTHORS, Pages 581-585
SUBJECT INDEX, Pages 587-589