Foodborne Bacterial Pathogens: Methods and Protocols

Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
 Accelerated Sample Preparation for Fast Salmonella Detection in Poultry Products  (Eduardo Ximenes, Seockmo Ku, Lori Hoagland, Michael R. Ladisch)....Pages 3-20
 Direct or DNA Extraction-Free Amplification and Quantification of Foodborne Pathogens  (Maggie R. Williams, Syed A. Hashsham)....Pages 21-33
 The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens  (Alejandro Garrido-Maestu, David Tomás Fornés, Marta Prado Rodríguez)....Pages 35-45
 Sequence-Specific End Labeling of Oligonucleotides (SSELO)-Based Microbial Detection  (Tanja Kostic, Levente Bodrossy)....Pages 47-56
 Rapid On-Site Detection and Quantification of Foodborne Pathogens Using Microfluidic Devices  (Nobuyasu Yamaguchi)....Pages 57-66
 Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria  (Matthew Dunne, Martin J. Loessner)....Pages 67-86
 EIS-Based Biosensors in Foodborne Pathogen Detection with a Special Focus on Listeria monocytogenes  (Palmiro Poltronieri, Elisabetta Primiceri, Rajeswaran Radhakrishnan)....Pages 87-101
Front Matter ....Pages 103-103
 Method to Study the Survival Abilities of Foodborne Bacterial Pathogens Under Food Processing Conditions  (Olivier Habimana)....Pages 105-116
 Viability Detection of Foodborne Bacterial Pathogens in Food Environment by PMA-qPCR and by Microscopic Observation  (Thomas Brauge, Graziella Midelet-Bourdin, Christophe Soumet)....Pages 117-128
 Isolation of Bacterial RNA from Foods Inoculated with Pathogens  (Deepti Tyagi, Autumn L. Kraft, Teresa M. Bergholz)....Pages 129-137
 Use of Two-Dimensional Electrophoresis to Explore Foodborne Bacterial Pathogen Responses to Gastrointestinal Stress  (Denise Schrama, Maria Leonor Faleiro)....Pages 139-147
 Identification of Putative Biomarkers Specific to Foodborne Pathogens Using Metabolomics  (Snehal R. Jadhav, Rohan M. Shah, Avinash V. Karpe, David J. Beale, Konstantinos A. Kouremenos, Enzo A. Palombo)....Pages 149-164
 Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells  (Florence Dubois-Brissonnet)....Pages 165-170
 High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 4D Structural Dynamic of Foodborne Pathogens  (Alexis Canette, Julien Deschamps, Romain Briandet)....Pages 171-182
 Static Immersion and Injection Methods for Live Cell Imaging of Foodborne Pathogen Infections in Zebrafish Larvae  (Macarena A. Varas, Javiera Ortíz-Severín, Andrés E. Marcoleta, Carlos A. Santiviago, Miguel L. Allende, Francisco P. Chávez)....Pages 183-190
 Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection  (Francisco P. Chávez, Andrea Calixto)....Pages 191-197
Front Matter ....Pages 199-199
 Utilizing the Public GenomeTrakr Database for Foodborne Pathogen Traceback  (Ruth E. Timme, Maria Sanchez Leon, Marc W. Allard)....Pages 201-212
 Establishment of a Standardized 16S rDNA Library Preparation to Enable Analysis of Microbiome in Poultry Processing Using Illumina MiSeq Platform  (Kristina M. Feye, Steven C. Ricke)....Pages 213-227
 Exploring Foodborne Pathogen Ecology and Antimicrobial Resistance in the Light of Shotgun Metagenomics  (Arnaud Bridier)....Pages 229-245
 Modeling Growth of Listeria and Lactic Acid Bacteria in Food Environments  (Paw Dalgaard, Ole Mejlholm)....Pages 247-264
 Network Biology Approaches to Identify Molecular and Systems-Level Differences Between Salmonella Pathovars  (Marton Olbei, Robert A. Kingsley, Tamas Korcsmaros, Padhmanand Sudhakar)....Pages 265-273
Back Matter ....Pages 275-278