Fluorescent Biomolecules: Methodologies and Applications

Front Matter....Pages i-x
Luminescence: General Concepts and Applications to the Study of Solids....Pages 1-16
Protein Dynamics: Fluorescence Lifetime Distributions....Pages 17-32
Enhancement of Time-Resolved Fluorescence Spectroscopy by Overdetermination....Pages 33-59
Fluorescence Investigations on the Elongation Factor Tu System....Pages 61-86
Effects on Intrinsic Fluorescence Induced by Active Site Modifications of Rhodanese....Pages 87-90
Lifetime Measurements of Color Centers by Fluorometry with Synchrotron Radiation....Pages 91-100
Slab Gel Electrophoresis Performed at High Hydrostatic Pressure: A New Approach for the Study of Oligomeric Proteins....Pages 101-113
Lipid Transfer Reactions: Fluorescence Studies....Pages 115-132
Thoughts and Hints on Possible Violation of Parity in the Biological Matter....Pages 133-138
Digitized Fluorescence Microscopy in Cell Biology Applications....Pages 139-149
Membrane Fluidity and Diffusive Transport....Pages 151-171
Conformational Flexibility of the Polypeptide Hormone Bombesin: Time Resolved and Static Fluorescence Studies....Pages 173-193
Usefulness of the Linkage Concept to Understanding the Regulation of Rat Liver Phosphofructokinase....Pages 195-216
Fluorescence Studies of the Calcium-Dependent Functions of Calmodulin....Pages 217-245
Definition of Monoclonal Antibody-Fluorescent Ligand Interactions....Pages 247-268
Intramolecular Excitation Energy Transfer in Bichromophoric Molecules - Fundamental Aspects and Applications....Pages 269-303
Resolution at 2 GHz of Lifetimes and Correlation Times of Highly Purified Solutions of Human Hemoglobin....Pages 305-318
Time-Resolved Fluorescence Studies on Flavins....Pages 319-341
Final Words at Bocca di Magra....Pages 343-349
Front Matter....Pages 351-351
A Non-Linear Least-Squares Approach to the Isothermal Perrin-Weber Plot in Terms of Rotational Correlation Times....Pages 353-356
Front Matter....Pages 351-351
Fluorescence Studies on Membranes from Human Skin Fibroblasts Deficient in Plasmalogens....Pages 357-359
Nanosecond Time-Resolved Fluorescence Studies of Thioredoxin....Pages 360-360
Analyzing the Distribution of Decay Constants in Pulse Fluorimetry Using the Maximum Entropy Method....Pages 361-363
Thermodynamic Properties of Antifluorescyl Antibodies....Pages 364-364
Applications of Fluorescent Probes for Monitoring Energy-Linked Phenomena in Biomembranes and Proteoliposomes....Pages 365-368
Fluorescence Quenching Studies on Enzyme I of the Bacterial Phosphotransferase System....Pages 369-373
Spontaneous Transfer and Molecular Organization of Dehydroergosterol in Lipid Bilayers....Pages 374-374
Standardization of Fluorophores Using Phase Fluorometry....Pages 375-377
Phasefluorometry Using the Harmonic Content of a Sync-Pumped Mode-Locked Cavity-Dumped Dye Laser....Pages 378-380
The Study of Tubulin-Colchicine Interactions Using Fluorescence Techniques....Pages 381-382
Structural Dynamics of Myelin Basic Protein in the Assembly of a Model Membrane....Pages 383-388
Analysis of Complex Fluorescence Decay Data....Pages 389-392
Time Resolved Fluorescence Depolarization of Dph in the Nematic Liquid Crystal ZLI-1167....Pages 393-396
Molecular Dynamics and Structure of Peptide Hormones at Membrane-Water Interfaces....Pages 397-400
Tryptophan Fluorescence as a Structural Probe of Interaction of Amphiphilic Peptides with Calmodulin....Pages 401-405
Fluorescence Decay Measurements of 1-Methylindole in Alcohol Solutions: Direct Observation of Solvent Reorientation Kinetics at Ambient Temperatures....Pages 406-410
Time-Resolved Fluorescence Studies on Horse Spleen Ferritin....Pages 411-413
Metal Substitution and Fluorescence Modifications in Ascorbate-Alkaline Phosphatase Interaction....Pages 414-418
Interactions Between Aminotransferases and Monoclonal Antibodies....Pages 419-422
Intramolecular Excimer Fluorescence of Pyrene Maleimide-Labeled Dithiothreitol (Pyrene-DTT)....Pages 423-425
Front Matter....Pages 351-351
Time-Resolved Fluorescence Energy Transfer Immunoassay....Pages 426-429
Porphyrin Motions in Mb desFe and Hb desFe ....Pages 429-429
Fluorescence Anisotropy Experiments on Hog Renal Brush Border Membrane Vesicles....Pages 430-432
Photophysics of the Tryptophan Residue in RNaseTl and RNase Tl-2′GMP Complex....Pages 432-432
The Effect of Phenylhydrazine on the Membrane Phospholipid Organization....Pages 433-434
The Use of Disulfonatonaphthalimide Fluorescent Dyes for the Fluorescence Polarization Immunoassay of Steroids....Pages 434-434
Following Inverted Micelle Behavior by Intrinsic Fluorescence Probes....Pages 435-436
Tryptophan Dynamics and Cooperativity in ATCase from E. coli ....Pages 436-436
Multifrequency Phase/Modulation Fluorescence Lifetime Measurements with Ribonuclease T 1 , Alcohol Dehydrogenase, and Selected Two-Tryptophan Proteins....Pages 437-439
Applications of the Uranyl Ion as a Fluorescent Probe in Model Biomembranes....Pages 440-441
Variation of 1,6-Diphenyl-1,3,5-Hexatriene Lifetime Distributions in Differentiating Proerythroblasts. A Multifrequency Phase and Modulation Fluorometry Study....Pages 442-445
Back Matter....Pages 447-461