Fluorescence Techniques in Cell Biology

Front Matter....Pages I-VIII
Opening Remarks....Pages 1-2
Front Matter....Pages 3-3
Polarized Fluorescence....Pages 5-13
Techniques for Fluorescence Lifetime Measurements and Time-Resolved Emission Spectroscopy....Pages 15-27
Front Matter....Pages 29-29
Some Aspects of Instrumentation and Methods as Applied to Fluorometry at the Microscale....Pages 31-39
Microcapillary Fluorometry and Standardization for Microscope Fluorometry....Pages 41-49
The Use of Human Leukocytes as a Standard for the Cytofluorometric Determination of Protein and DNA....Pages 51-55
A Microspectrofluorometer for Measuring Spectra of Excitation, Emission and Absorption in Cells and Tissues....Pages 57-63
Front Matter....Pages 65-65
Absorbance and Fluorescence Cytophotometry of Nuclear Feulgen DNA. A Comparative Study....Pages 67-77
Automation of Cytofluorometry by Use of the Impulsmicrophotometer....Pages 79-88
Quantitative Determination of DNA and Protein in Single Cells....Pages 89-93
Fluorescence Properties of the Monoaminoacridines and Some 2-Aminoacridine-Derivatives....Pages 95-105
Fluorometric Recognition of Chromosomes and Chromosome Regions....Pages 107-123
Chemical Aspects of the Fluorescence Analysis of Chromosomes....Pages 125-134
Fluorescence Cytochemical Studies of Chromosomes: Quantitative Applications of Fluorescein Mercuric Acetate....Pages 135-149
Microfluorimetric Comparison of Chromatin during Cytodifferentiation....Pages 151-162
Immunofluorometric Determinations of Surface Immunoglobulins of Individual Normal and Malignant Human Lymphoid Cells....Pages 163-170
Cytofluorometry of Biogenic Monoamines in the Falck-Hillarp Method. Structural Identification by Spectral Analysis....Pages 171-181
Microfluorimetric Quantitation of Biogenic Monoamines....Pages 183-189
Quantitation of Biogenic Monoamines Demonstrated with the Formaldehyde Fluorescence Method....Pages 191-197
The Identification of Arylethylamines by Microspectrofluorometry of Acid- and Aldehyde-Induced Fluorescence....Pages 199-204
Front Matter....Pages 205-205
Quantitative Aspects of Rapid Microfluorometry for the Study of Enzyme Reactions and Transport Mechanisms in Single Living Cells....Pages 207-218
Rapid Microfluorometry for Biochemistry of the Living Cell in Correlation with Cytomorphology and Transport Phenomena....Pages 219-233
Newer Fluorometric Methods for the Analysis of Biologically Important Compounds....Pages 235-242
Microfluorometric Investigations on the Intracellular Turnover of Fluorogenic Substrates....Pages 243-254
Changes in the Membrane Permeability of Human Leukocytes Measured by Fluorochromasia in a Rapid Flow Fluorometer....Pages 255-258
Front Matter....Pages 259-259
Probes for Enzyme Conformation....Pages 261-272
The Fluorescence of Bilirubin-Albumin Complexes....Pages 273-282
The Effect of the Excitation Wavelength on the Fluorescence Depolarization of Protein-Dye Complexes or Conjugates....Pages 283-290
Fluorescence, Absorption, and Optical Rotatory Behavior of Acridine Orange: Poly-L-Glutamic Acid Complexes....Pages 291-299
Ethidium Bromide: A Fluorescent Probe of Nucleic Acid Structure and Its Potential for in-Vivo Studies....Pages 301-309
Nanosecond Fluorescence Spectroscopy of Biological Macromolecules and Membranes....Pages 311-331
Measurements of Single Molecules of Antibody by their Ability to Activate a Defective Enzyme....Pages 333-337
Nanosecond Time-Resolved Fluorescence Spectroscopy in Molecular Biology....Pages 339-344
Fluorescence Spectroscopy as a Tool for Studying Drug Interactions with Biological Systems....Pages 345-357
Temperature Sensitivity of Fluorescent Probes in the Presence of Model Membranes and Mitochondria....Pages 359-365
Conformational Dynamics of Model Membranes....Pages 367-379
Atebrin and Related Fluorochromes as Quantitative Probes of Membrane Energization....Pages 381-394
Fluorescent Molecular Probes in Fluorescence Microspectrophotometry and Microspectropolarimetry....Pages 395-407
Back Matter....Pages 409-422