Fluorescence Microscopy of Living Cells in Culture Part B. Quantitative Fluorescence Microscopy—Imaging and Spectroscopy

Content: 
Series Editor
Page ii

Edited by
Page iii

Copyright Page
Page iv

Colour Plate
Page x

Contributors
Pages xi-xii

Preface
Pages xiii-xiv
D. Lansing Taylor, Yu-Li Wang

Chapter 1 Image Fidelity: Characterizing the Imaging Transfer Function Original Research Article
Pages 1-45
Ian T. Young

Chapter 2 Validation of an Imaging System: Steps to Evaluate and Validate a Microscope Imaging System for Quantitative Studies Original Research Article
Pages 47-83
Željko Jeričević, Brent Wiese, Joseph Bryan, Louis C. Smith

Chapter 3 Imaging of Unresolved Objects, Superresolution, and Precision of Distance Measurement with Video Microscopy Original Research Article
Pages 85-112
Shinya Inoué

Chapter 4 Fluorescent Standards Original Research Article
Pages 113-126
Jesse E. Sisken

Chapter 5 Fluorescent Indicators of Ion Concentrations Original Research Article
Pages 127-156
Roger Y. Tsien

Chapter 6 Fluorescence Ratio Imaging Microscopy Original Research Article
Pages 157-192
G.R. Bright, G.W. Fisher, J. Rogowska, D.L. Taylor

Chapter 7 Fluorescent Indicators of Membrane Potential: Microspectrofluorometry and Imaging Original Research Article
Pages 193-218
David Gross, Leslie M. Loew

Chapter 8 Resonance Energy Transfer Microscopy Original Research Article
Pages 219-243
Brian Herman

Chapter 9 Total Internal Reflection Fluorescence Microscopy Original Research Article
Pages 245-270
Daniel Axelrod

Chapter 10 Designing, Building, and Using a Fluorescence Recovery after Photobleaching Instrument Original Research Article
Pages 271-306
David E. Wolf

Chapter 11 Interpretation of Fluorescence Correlation Spectroscopy and Photobleaching Recovery in Terms of Molecular Interactions Original Research Article
Pages 307-332
Elliot. L. Elson, Hong Qian

Chapter 12 Fluorescence Polarization Microscopy Original Research Article
Pages 333-352
Daniel Axelrod

Chapter 13 Fluorescence Microscopy in Three Dimensions Original Research Article
Pages 353-374,374a,375-377
David A. Agard, Yasushi Hiraoka, Peter Shaw, John W. Sedat

Chapter 14 Three-Dimensional Confocal Fluorescence Microscopy Original Research Article
Pages 379-398
G.J. Brakenhoff, E.A. Van Spronsen, H.T.M. Van Der Voort, N. Nanninga

Chapter 15 Emission of Fluorescence at an Interface Original Research Article
Pages 399-416
Daniel Axelrod, Edward H. Hellen

Chapter 16 Fluorescence Labeling and Microscopy of DNA Original Research Article
Pages 417-448
Donna J. Arndt-Jovin, Thomas M. Jovin

Chapter 17 Multiple Spectral Parameter Imaging Original Research Article
Pages 449-478
A. Waggoner, R.De Biasio, P. Conrad, G.R. Bright, L. Ernst, K. Ryan, M. Nederlof, D. Taylor

Index
Pages 479-498