Fluorescence-Based Biosensors: From Concepts to Applications

Content: 
Copyright
Page iv

Contributors
Pages ix-xi

Preface
Page xiii
May C. Morris

Introduction
Pages xv-xviii

Chapter One - The Chemistry of Small-Molecule Fluorogenic Probes
Pages 1-34
Jonathan B. Grimm, Laurel M. Heckman, Luke D. Lavis

Chapter Two - Fluorescent Environment-Sensitive Dyes as Reporters of Biomolecular Interactions
Pages 35-58
Andrey S. Klymchenko, Yves. Mely

Chapter Three - Rational Design of Fluorophores for In Vivo Applications
Pages 59-108
Marcin. Ptaszek

Chapter Four - Fluorescence Technologies for Monitoring Interactions Between Biological Molecules In Vitro
Pages 109-143
Sebastien. Deshayes, Gilles. Divita

Chapter Five - From FRET Imaging to Practical Methodology for Kinase Activity Sensing in Living Cells
Pages 145-216
François Sipieter, Pauline Vandame, Corentin Spriet, Aymeric Leray, Pierre Vincent, Dave Trinel, Jean-François Bodart, Franck B. Riquet, Laurent Héliot

Chapter Six - Fluorescent Sensors of Protein Kinases: From Basics to Biomedical Applications
Pages 217-274
Thi Nhu Ngoc Van, May C. Morris

Chapter Seven - Time-Resolved Förster Resonance Energy Transfer-Based Technologies to Investigate G Protein-Coupled Receptor Machinery: High-Throughput Screening Assays and Future Development
Pages 275-312
Pauline Scholler, Jurriaan M. Zwier, Eric Trinquet, Philippe Rondard, Jean-Philippe Pin, Laurent Prézeau, Julie Kniazeff

Chapter Eight - Fluorescent Protein-Based Biosensors and Their Clinical Applications
Pages 313-348
Yusuke Ohba, Yoichiro Fujioka, Shigeyuki Nakada, Masumi Tsuda

Chapter Nine - Fluorescent Macromolecular Sensors of Enzymatic Activity for In Vivo Imaging
Pages 349-387
Alexei A. Bogdanov, Mary L. Mazzanti

Chapter Ten - Fluorescent Proteins as Visible In Vivo Sensors
Pages 389-402
Robert M. Hoffman

Index
Pages 403-414