Flow Cytometry Second Edition, Part A

Content: 
Series Editors
Page ii

Edited by
Page ii

Copyright Page
Page iv

Dedication
Page v

Contributors
Pages xxi-xxiii

Preface to the Second Edition
Pages xxv-xxvii
Zbigniew Darzynkiewicz, J. Paul Robinson, Harry A. Crissman

Preface to the First Edition
Pages xxix-xxxi
Zbigniew Darzynkiewicz, Harry A. Crissman

Chapter 1 Dissociation of Intact Cells from Tumors and Normal Tissues Original Research Article
Pages 1-13
Daniel W. Visscher, John D. Crissman

Chapter 2 Assays of Cell Viability: Discrimination of Cells Dying by Apoptosis Original Research Article
Pages 15-38
Zbigniew Darzynkiewicz, Xun Li, Jianping Gong

Chapter 3 Cell Preparation for the Identification of Leukocytes Original Research Article
Pages 39-60
Carleton C. Stewart, Sigrid J. Stewart

Chapter 4 Multiparameter Analysis of Leukocytes by Flow Cytometry Original Research Article
Pages 61-79
Carleton C. Stewart, Sigrid J. Stewart

Chapter 5 Immunophenotyping Using Fixed Cells Original Research Article
Pages 81-93
George F. Babcock, Susan M. Dawes

Chapter 6 Simultaneous DNA Content and Cell-Surface Immunofluorescence Analysis Original Research Article
Pages 95-102
Paul P.T. Brons, Piet E.J. Van Erp, Arie H.M. Pennings

Chapter 7 Flow Cytometry Crossmatching for Solid Organ Transplantation Original Research Article
Pages 103-119
Robert A. Bray

Chapter 8 Cell Membrane Potential Analysis Original Research Article
Pages 121-133
Howard M. Shapiro

Chapter 9 Measurement of Intracellular pH Original Research Article
Pages 135-148
Michael J. Boyer, David W. Hedley

Chapter 10 Intracellular Ionized Calcium Original Research Article
Pages 149-174
Carl H. June, Peter S. Rabinovitch

Chapter 11 Cellular Protein Content Measurements Original Research Article
Pages 175-183
Harry A. Crissman, John A. Steinkamp

Chapter 12 Lysosomal Proton Pump Activity: Supravital Cell Staining with Acridine Orange Differentiates Leukocyte Subpopulations Original Research Article
Pages 185-194
Frank Traganos, Zbigniew Darzynkiewicz

Chapter 13 Staining of DNA in Live and Fixed Cells Original Research Article
Pages 195-209
Harry A. Crissman, Gregory T. Hirons

Chapter 14 High-Resolution Analysis of Nuclear DNA Employing the Fluorochrome DAPI Original Research Article
Pages 211-217
Friedrich J. Otto

Chapter 15 Detergent and Proteolytic Enzyme-Based Techniques for Nuclear Isolation and DNA Content Analysis Original Research Article
Pages 219-229
Lars L. Vindeløv, Ib Jarle Christensen

Chapter 16 DNA Analysis from Paraffin-Embedded Blocks Original Research Article
Pages 231-240
David W. Hedley

Chapter 17 Controls, Standards, and Histogram Interpretation in DNA Flow Cytometry Original Research Article
Pages 241-262
Lynn G. Dressler, Larry C. Seamer

Chapter 18 DNA Content Histogram and Cell-Cycle Analysis Original Research Article
Pages 263-296
Peter S. Rabinovitch

Chapter 19 Immunochemical Quantitation of Bromodeoxyuridine: Application to Cell–Cycle Kinetics Original Research Article
Pages 297-316
Frank Dolbeare, Jules R. Selden

Chapter 20 Application and Detection of IdUrd and CldUrd as Two Independent Cell–Cycle Markers Original Research Article
Pages 317-326
Jacob A. Aten, Jan Stap, Ron Hoebe, Piet J.M. Bakker

Chapter 21 Cell-Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33358—Ethidium Bromide Bivariate Flow Cytometry Original Research Article
Pages 327-340
Martin Poot, Holger Hoehn, Manfred Kubbies, Angelika Grossmann, Yuchyau Chen, Peter S. Rabinovitch

Chapter 22 Detection of BrdUrd-Labeled Cells by Differential Fluorescence Analysis of DNA Fluorochromes: Pulse-Chase and Continuous Labeling Methods Original Research Article
Pages 341-349
Harry A. Crissmaan, Noboru Oishi, Robert Habbersett

Chapter 23 Analysis of Intracellular Proteins Original Research Article
Pages 351-376
Kenneth D. Bauer, James W. Jacobberger

Chapter 24 “Washless” Procedures for Nuclear Antigen Detection Original Research Article
Pages 377-388
Jøgen K. Larsen

Chapter 25 Light Scatter of Isolated Cell Nuclei as a Parameter Discriminating the Cell-Cycle Subcompartments Original Research Article
Pages 389-400
Walter Giaretti, Michael Nüsse

Chapter 26 Simultaneous Analysis of Cellular RNA and DNA Content Original Research Article
Pages 401-420
Zbigniew Darzynkiewicz

Chapter 27 Analysis of DNA Content and Cyclin Protein Expression in Studies of DNA Ploidy, Growth Fraction, Lymphocyte Stimulation, and the Cell Cycle Original Research Article
Pages 421-435
Zbigniew Darzynkiewicz, Jianping Gong, Frank Traganos

Chapter 28 Oxidative Product Formation Analysis by Flow Cytometry Original Research Article
Pages 437-447
J. Paul Robinson, Wayne O. Carter, Padma Kumar Narayanan

Chapter 29 Flow Cytometric Determination of Cysteine and Serine Proteinase Activities in Living Cells with Rhodamine 110 Substrates Original Research Article
Pages 449-459
Sven Klingel, Gregor Rothe, Wolfgang Kellermann, Günter Valet

Chapter 30 Leucine Aminopeptidase Activity by Flow Cytometry Original Research Article
Pages 461-467
John J. Turek, J. Paul Robinson

Chapter 31 Enzyme Kinetics Original Research Article
Pages 469-507
James V. Watson, Caroline Dive

Chapter 32 On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements Original Research Article
Pages 509-525
Kees Nooter, Hans Herweijer, Richard R. Jonker, Ger J. van den Engh

Chapter 33 Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin Structure Original Research Article
Pages 527-541
Zbigniew Darzynkiewicz

Chapter 34 Detection of Intracellular Virus and Viral Products Original Research Article
Pages 543-557
Judith Laffin, John M. Lehman

Index
Pages 559-586

Volumes In Series
Pages 587-591