Flow Cytometry

Content: 
Series Editor
Page ii

Editorial Page
Page iii

Copyright Page
Page iv

Contributors
Pages xi-xiv

Preface
Pages xv-xvii
Zbigniew Darzynkiewicz, Harry A. Crissman

Chapter 1 Dissociation of Cells from Solid Tumors Original Research Article
Pages 1-12
Robert Cerra, Richard J. Zarbo, John D. Crissman

Chapter 2 Flow Cytometric Measurement of Cell Viability Using DNase Exclusion Original Research Article
Pages 13-18
Oskar S. Frankfurt

Chapter 3 Cell Cycle Phase-Specific Analysis of Cell Viability Using Hoechst 33342 and Propidium Iodide after Ethanol Preservation Original Research Article
Pages 19-24
Alan Pollack, Gaetano Ciancio

Chapter 4 Cell Membrane Potential Analysis Original Research Article
Pages 25-35
Howard M. Shapiro

Chapter 5 Flow Cytometric Measurement of Intracellular Ionized Calcium in Single Cells with Indo-1 and Fluo-3 Original Research Article
Pages 37-58
Carl H. June, Peter S. Rabinovitch

Chapter 6 Measurement of Intracellular pH Original Research Article
Pages 59-69
Elizabeth A. Musgrove, David W. Hedley

Chapter 7 Flow Cytometric Techniques for Measurement of Cytochrome P-450 Activity in Viable Cells Original Research Article
Pages 71-79
A. Dusty Miller

Chapter 8 Fluorescent Staining of Enzymes for Flow Cytometry Original Research Article
Pages 81-88
Frank Dolbeare

Chapter 9 Supravital Cell Staining with Hoechst 33342 and DiOC5(3) Original Research Article
Pages 89-95
Harry A. Crissman, Marianne H. Hofland, Anita P. Stevenson, Mark E. Wilder, Robert A. Tobey

Chapter 10 Specific Staining of DNA with the Fluorescent Antibiotics, Mithramycin, Chromomycin, and Olivomycin Original Research Article
Pages 97-103
Harry A. Crissman, Robert A. Tobey

Chapter 11 DAPI Staining of Fixed Cells for High-Resolution Flow Cytometry of Nuclear DNA Original Research Article
Pages 105-110
Friedrich Otto

Chapter 12 High-Resolution DNA Measurements Using the Nuclear Isolation Medium, DAPI, with the RATCOM Flow Cytometer Original Research Article
Pages 111-119
Jerry T. Thornthwaite, Richard A. Thomas

Chapter 13 Rapid DNA Content Analysis by the Propidium Iodide–Hypotonic Citrate Method Original Research Article
Pages 121-125
Awtar Krishan

Chapter 14 An Integrated Set Of Methods For Routine Flow Cytometric DNA Analysis Original Research Article
Pages 127-137
Lars Vindeløv, IB Jarle Christensen

Chapter 15 DNA Analysis from Paraffin-Embedded Blocks Original Research Article
Pages 139-147
David W. Hedley

Chapter 16 Detection of M and Early-G1 Phase Cells by Scattering Signals Combined with Identification of G1 S, and G2Phase Cells Original Research Article
Pages 149-156
Elio Geido, Walter Giaretti, Michael N¨Sse

Chapter 17 Controls, Standards, and Histogram Interpretation in DNA Flow Cytometry Original Research Article
Pages 157-171
Lynn G. Dressler

Chapter 18 Cell Division Analysis Using Bromodeoxyuridine-Induced Suppressionof Hoechst 33258 Fluorescence Original Research Article
Pages 173-184
Ralph M. B¨ohmer

Chapter 19 Cell Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33258-Ethidium Bromide Bivariate Flow Cytometry Original Research Article
Pages 185-198
Martin Poot, Holger Hoehn, Manfred Kubbies, Angelika Grossmann, Yuhchyau Chen, Peter S. Rabinovitch

Chapter 20 Detection of Bromodeoxyuridine-Labeled Cells by Differential Fluorescence Analysis of DNA Fluorochromes Original Research Article
Pages 199-206
Harry A. Crissman, John A. Steinkamp

Chapter 21 Using Monoclonal Antibodies in Bromodeoxyuridine—DNA Analysis Original Research Article
Pages 207-216
Frank Dolbeare, Wen-Lin Kuo, Wolfgang Beisker, Martin Vanderlaan, Joe W. Gray

Chapter 22 dentification of Proliferating Cells by Ki-67 Antibody Original Research Article
Pages 217-226
Heinz Baisch, Johannes Gerdes

Chapter 23 Washless Double Staining of a Nuclear Antigen (Ki-67 or Bromodeoxyuridine) and DNA in Unfixed Nuclei Original Research Article
Pages 227-234
Jørgen K. Larsen

Chapter 24 Analysts of Proliferation-Associated Antigens Original Research Article
Pages 235-247
Kenneth D. Bauer

Chapter 25 The Stathmokinetic Experiment: A Single-Parameter and Multiparameter Flow Cytometric Analysis Original Research Article
Pages 249-270
Frank Traganos, Marek Kimmel

Chapter 26 Detection of Intracellular Virus and Viral Products Original Research Article
Pages 271-284
Judith Laffin, John M. Lehman

Chapter 27 Differential Staining of DNA and RNA in Intact Cells and Isolated Cell Nuclei with Acridine Orange Original Research Article
Pages 285-298
Zbigniew Darzynkiewicz

Chapter 28 Flow Cytometric Analysis of Double-Stranded RNA Content Distributions Original Research Article
Pages 299-304
Oskar S. Frankfurt

Chapter 29 Simultaneous Fluorescent Labeling of DNA, RNA, and Protein Original Research Article
Pages 305-314
Harry A. Crissman, Zbigniew Darzynkiewicz, John A. Steinkamp, Robert A. Tobey

Chapter 30 Flow Cytometric Cell-Kinetic Analysis by Simultaneously Staining Nuclei with Propidium Iodide and Fluorescein Isothiocyanate Original Research Article
Pages 315-323
Alan Pollack

Chapter 31 Flow Cytometric Methods for Studying Isolated Nuclei: DNA Accessibility to DNase I and Protein–DNA Content Original Research Article
Pages 325-336
Ryuji Higashikubo, William D. Wright, Joseph L. Roti Roti

Chapter 32 Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin Structure Original Research Article
Pages 337-352
Zbigniew Darzynkiewicz

Chapter 33 Fluorescent Methods for Studying Subnuclear Particles Original Research Article
Pages 353-362
William D. Wright, Ryuji Higashikubo, Joseph L. Roti Roti

Chapter 34 Chromosome and Nuclei Isolation with the MgSO4 Procedure Original Research Article
Pages 363-367
Barbara Trask, Ger Van Den Engh

Chapter 35 Univariate Analysis of Metaphase Chromosomes Using the Hypotonic Potassium Chloride-Propidium Iodide Protocol Original Research Article
Pages 369-376
L.Scott Cram, Frank A. Ray, Marty F. Bartholdi

Chapter 36 Polyamine Buffer for Bivariate Human Flow Cytogenetic Analysis and Sorting Original Research Article
Pages 377-382
L. Scott Cram, Mary Campbell, John J. Fawcett, Larry L. Deaven

Chapter 37 Fluorescence in Situ Hybridization with DNA Probes Original Research Article
Pages 383-400
Barbara Trask, Dan Pinkel

Chapter 38 Flow Cytometric Analysis of Male Germ Cell Quality Original Research Article
Pages 401-410
Donald P. Evenson

Chapter 39 Cell Preparation for the Identification of Leukocytes Original Research Article
Pages 411-426
Carleton C. Stewart

Chapter 40 Multiparameter Analysis of Leukocytes by Flow Cytometry Original Research Article
Pages 427-450
Carleton C. Stewart

Chapter 41 Identafication and Purafication of Murine Hematopoietic Stem Cellsby Flow Cytometry Original Research Article
Pages 451-468
Jan W.M. Visser, Peter De Vries

Chapter 42 Fluorescent Cell Labeling for in Vivo and in Vitro Cell Tracking Original Research Article
Pages 469-490
Paul Karl Horan, Meryle J. Melnicoff, Bruce D. Jensen, Sue E. Slezak

Chapter 43 Rapid Determination of Cellular Resistance-Related Drug Efflux in Tumor Cells Original Research Article
Pages 491-500
Awtar Krishan

Chapter 44 Slit-Scan Flow Analysis of Cytologic Specimens from the Female Genital Tract Original Research Article
Pages 501-507
Leon L. Wheeless Jr., Jay E. Reeder, Mary J. O'Connell

Chapter 45 Cell Sorting with Hoechst or Carbocyanine Dyes as Perfmion Probes in Spheroids and Tumors Original Research Article
Pages 509-518
Ralph E. Durand, David J. Chaplin, Peggy L. Olive

Chapter 46 DNA Measurements of Bacteria Original Research Article
Pages 519-526
Harald B. Steen, Kirsten Skarstad, Erik Boye

Chapter 47 Isolation and Flow Cytometric Characterization of Plant Protoplasts Original Research Article
Pages 527-547
David W. Galbraith

Chapter 48 Flow Cytometric Analysis of Plant Genomes Original Research Article
Pages 549-562
David W. Galbraith

Chapter 49 Plant Cell Cycle Analysis with Isolated Nuclei Original Research Article
Pages 563-573
Catherine Bergounioux, Spencer C. Brown

Chapter 50 Environmental Health: Flow Cytometric Methods to Assess Our Water World Original Research Article
Pages 575-612
Clarice M. Yentsch

Chapter 51 Flow Microsphere Immunoassay for the Quantitative and SimultaneousDetection of Multiple Soluble Analytes Original Research Article
Pages 613-629
Mack J. Fulwyler, Thomas M. McHugh

Chapter 52 On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements Original Research Article
Pages 631-645
Kees Nooter, Hans Herweijer, Richard Jonker, Ger Van Den Engh

Chapter 53 Calibration of Flow Cytometer Detector Systems Original Research Article
Pages 647-654
Ralph E. Durand

Chapter 54 Spectral Properties of Fluorochromes Used in Flow Cytometry Original Research Article
Pages 655-669
Jan Kapuscinski, Zbigniew Darzynkiewicz

Index
Pages 671-709

Contents of Recent Volulmes
Pages 711-716