Fingerprinting Methods Based on Arbitrarily Primed PCR

Front Matter....Pages I-XVI
Front Matter....Pages 1-1
Overview....Pages 3-13
DNA Extraction from Mammals....Pages 15-20
Insect DNA Extraction Protocol....Pages 21-24
Rapid DNA Extraction from Plants....Pages 25-28
Preparation of Fungal Genomic DNA for PCR and RAPD Analysis....Pages 29-34
Extraction of Histoplasma capsulatum DNA for PCR....Pages 35-40
DNA Extraction from Bacterial Cultures....Pages 41-45
Arbitrarily Primed PCR and RAPDs....Pages 47-53
Random Amplified Polymorphic DNA Assay....Pages 55-63
DNA Amplification Fingerprinting....Pages 65-80
Fluorescent Detection and Analysis of RAPD Amplicons Using the ABI PRISM DNA Sequencers....Pages 81-92
Optimization of RAPD Fingerprinting....Pages 93-102
Fingerprint Tailoring....Pages 103-117
Resolving DNA Amplification Products Using Polyacrylamide Gel Electrophoresis and Silver Staining....Pages 119-134
Denaturing Gradient Gel Electrophoresis for Enhanced Detection of DNA Polymorphisms....Pages 135-141
Modified Temperature Sweep Gel Electrophoresis for the Separation of Arbitrarily Amplified DNA Fragments....Pages 143-148
High Throughput Scoring of RAPD Fragments Through the Use of Dot-Blot Hybridization....Pages 149-152
Recovering Amplified DNA from Silver Stained Gels....Pages 153-160
Cloning of RAPD Markers....Pages 161-169
Sequencing of RAPD Markers....Pages 171-176
Front Matter....Pages 1-1
Analysis of Tumor-Specific Genetic Alterations by Arbitrarily Primed PCR....Pages 177-185
Construction of Linkage Maps with RAPD Markers....Pages 187-199
Pseudo-Testcross Mapping Strategy Using RAPD Markers....Pages 201-218
Estimating Nucleotide Divergence with RAPD Data....Pages 219-225
RAPD and PAUP Analysis for Microbial Screening Programs....Pages 227-241
Production of Specific Probes for Microorganisms....Pages 243-253
Front Matter....Pages 255-255
Overview....Pages 257-268
Differential Display of Expressed mRNAs....Pages 269-281
RNA Arbitrarily Primed PCR....Pages 283-293
Nonradioactive Differential Display of Messenger RNA....Pages 295-304
Fluorescent Differential Display: A Fast and Safe Way for Reliable Differential Display Analysis....Pages 305-313
Slot Blot Hybridization Screening....Pages 315-327
How To Find and Clone the Appropriate cDNA Fragments Generated in Differential mRNA Display by Using Northern Blot for cDNA Capture....Pages 329-336
Verification of Differential Display Results by RNase Protection....Pages 337-343
Direct Automated Sequencing of DDRT-PCR Fragments....Pages 345-351
Ligation Linked PCR and Direct Sequencing of Differential Display Products....Pages 353-358
Differential Display PCR Fragments as Probes for cDNA Cloning....Pages 359-369
Targeted RNA Fingerprinting....Pages 371-387
Generation Of Tumor-Specific DEST Catalogues....Pages 389-403
Analysis of Gene Expression in the Preimplantation Mouse Embryo Using mRNA Differential Display....Pages 405-414
Front Matter....Pages 255-255
Rapid Identification of Differentially Expressed Genes in Trypanosomes....Pages 415-434
Back Matter....Pages 435-441