Fibrosis : methods and protocols

Front Matter ....Pages i-xv
 Human Fibrotic Diseases: Current Challenges in Fibrosis Research  (Joel Rosenbloom, Edward Macarak, Sonsoles Piera-Velazquez, Sergio A. Jimenez)....Pages 1-23
Front Matter ....Pages 25-25
 The Bleomycin Model of Pulmonary Fibrosis  (Tianju Liu, Francina Gonzalez De Los Santos, Sem H. Phan)....Pages 27-42
 Intradermal Injections of Bleomycin to Model Skin Fibrosis  (Toshiyuki Yamamoto)....Pages 43-47
 Assessing the Effects of Fibrosis on Lung Function by Light Microscopy-Coupled Stereology  (Bartosz Pilecki, Grith Lykke Sørensen)....Pages 49-63
 Transplanting Human Skin Grafts onto Nude Mice to Model Skin Scars  (Jie Ding, Edward E. Tredget)....Pages 65-80
 Hypertrophic Scarring in the Rabbit Ear: A Practical Model for Studying Dermal Fibrosis  (Layla Nabai, Aziz Ghahary)....Pages 81-89
 Mouse and Rat Models of Induction of Hepatic Fibrosis and Assessment of Portal Hypertension  (Sabine Klein, Robert Schierwagen, Frank Erhard Uschner, Jonel Trebicka)....Pages 91-116
 Mouse Models of Corneal Scarring  (Laure Rittié, Audrey E. K. Hutcheon, James D. Zieske)....Pages 117-122
 Modeling Cardiac Fibrosis in Mice: (Myo)Fibroblast Phenotype After Ischemia  (Daniela Fraccarollo, Paolo Galuppo, Johann Bauersachs)....Pages 123-137
 Characterization of Mesenchymal-Fibroblast Cells Using the Col1a2 Promoter/Enhancer  (Ian M. H. Li, Amy L. Horwell, Grace Chu, Benoit de Crombrugghe, George Bou-Gharios)....Pages 139-161
Front Matter ....Pages 163-163
 Isolation and Culture of Primary Murine Hepatic Stellate Cells  (Sabine Weiskirchen, Carmen G. Tag, Sibille Sauer-Lehnen, Frank Tacke, Ralf Weiskirchen)....Pages 165-191
 Isolation and Culture of Adipose-Derived Stromal Cells from Subcutaneous Fat  (Lawrence J. Liew, Huan Ting Ong, Rodney J. Dilley)....Pages 193-203
 Isolation of Live Fibroblasts by Fluorescence-Activated Cell Sorting  (Tripp Leavitt, Michael S. Hu, Michael T. Longaker)....Pages 205-212
 Detection of Infiltrating Mast Cells Using a Modified Toluidine Blue Staining  (Nahum Puebla-Osorio, Seri N. E. Sarchio, Stephen E. Ullrich, Scott N. Byrne)....Pages 213-222
 Cell-Populated Collagen Lattice Models  (Beate Eckes, Fang Wang, Laure Rittié, Gabriele Scherr, Paola Zigrino)....Pages 223-233
 Traction Force Measurement Using Deformable Microposts  (Tianfa Xie, Jamar Hawkins, Yubing Sun)....Pages 235-244
 Mechanical Deformation of Cultured Cells with Hydrogels  (Christal A. Worthen, Laure Rittié, Gary J. Fisher)....Pages 245-251
 Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis  (Franziska E. Uhl, Darcy E. Wagner, Daniel J. Weiss)....Pages 253-283
Front Matter ....Pages 285-285
 Type I Collagen Purification from Rat Tail Tendons  (Laure Rittié)....Pages 287-308
 Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments  (Giampiero Pietrocola, Simonetta Rindi, Giulia Nobile, Pietro Speziale)....Pages 309-324
 Laser Capture Microdissection of Tissue Sections for High-Throughput RNA Analysis  (Julia Hoffmann, Jochen Wilhelm, Grazyna Kwapiszewska)....Pages 325-340
 Collagen Quantification in Tissue Specimens  (João Quintas Coentro, Héctor Capella-Monsonís, Valeria Graceffa, Zhuning Wu, Anne Maria Mullen, Michael Raghunath et al.)....Pages 341-350
 Methods for the Assessment of Active Transforming Growth Factor-β in Cells and Tissues  (Alison E. John, Joanne Porte, Gisli Jenkins, Amanda L. Tatler)....Pages 351-365
 Visualizing In Vitro Type I Collagen Fibrillogenesis by Transmission Electron Microscopy  (J. Robin Harris)....Pages 367-383
 Histological and Electron Microscope Staining for the Identification of Elastic Fiber Networks  (Elaine C. Davis, Ling Li)....Pages 385-393
 Method for Picrosirius Red-Polarization Detection of Collagen Fibers in Tissue Sections  (Laure Rittié)....Pages 395-407
 Probing Collagen Organization: Practical Guide for Second-Harmonic Generation (SHG) Imaging  (Riccardo Cicchi, Francesco S. Pavone)....Pages 409-425
Front Matter ....Pages 427-427
 Methods for Quantifying Fibrillar Collagen Alignment  (Yuming Liu, Adib Keikhosravi, Guneet S. Mehta, Cole R. Drifka, Kevin W. Eliceiri)....Pages 429-451
 Exploring the Nano-Surface of Collagenous and Other Fibrotic Tissues with AFM  (Andreas Stylianou, Vasiliki Gkretsi, Costas S. Patrickios, Triantafyllos Stylianopoulos)....Pages 453-489
 Spectral Unmixing Methods and Tools for the Detection and Quantitation of Collagen and Other Macromolecules in Tissue Specimens  (Zachary T. Harmany, Farzad Fereidouni, Richard M. Levenson)....Pages 491-509
 Simple Analysis of Deposited Gene Expression Datasets for the Non-Bioinformatician: How to Use GEO for Fibrosis Research  (Yang Guo, Richard Townsend, Lam C. Tsoi)....Pages 511-525
Back Matter ....Pages 527-530