Experimental and Applied Physiology Laboratory Manual

Preface......Page 1
To The Student......Page 2
1. Metrics, Measurements, and Computations......Page 4
2. Bioinstrumentation......Page 16
3. Compound Light Microscopy......Page 34
4. Physical Processes of Biological Importance......Page 42
5. Neural Control of Skeletal Muscle......Page 54
6. Contractility of Skeletal Muscle I: The Twitch and Motor Unit Recruitment......Page 65
7. Contractility of Skel. Muscle II: Mech. Sum., Contracture, Tetanus & Fatigue......Page 81
8. Contractility of Skel. Muscle III: Iso. Contraction & Initial Length vs. Work......Page 95
9. Electromyography and Dynamometry......Page 112
10. Neuromuscular Reflexes of the Spinal Cord and Brain Stem......Page 119
11. Somatic Sensation......Page 133
12. Cranial Nerves: Assessment of Functions......Page 141
13. Visual Acuity, Accom., Peripheral Vision, Color Vision, and Ophthalmoscopy......Page 155
14. Hearing and Equilibrium......Page 171
15. Electroencephalography I: Relaxation and Brain Rhythms......Page 187
16. Electroencephalography II: Occipital Lobe Alpha Rhythms......Page 199
17. Blood Cells and Blood Types......Page 209
18. Red Blood Cell Count......Page 222
19. Hemoglobin Content, Hematocrit, and Red Cell Indices......Page 230
20. Hemostasis: The Platelet Count, Bleeding Time, and Coagulation Time......Page 238
21. White Blood Cell Count......Page 248
22. Microcirculation......Page 259
23. The Cardiac Cycle......Page 265
24. Heart Sounds, Pulse Rate, and Systemic Blood Pressure......Page 281
25. Plethysmography and the Peripheral Pressure Pulse......Page 293
26. Electrical Activity of the Heart: The Electrocardiogram......Page 301
28. The Respiratory Cycle......Page 323
29. Pulmonary Function Tests: Volumes and Capacities......Page 336
30. Pulmonary Function Tests: Forced Expiratory Vol. & Maximal Voluntary Vent.......Page 352
31. Analysis of Urine......Page 364
32. Digestion......Page 376
33. Metabolic Rates: Indirect Calorimetry......Page 384
34. Maintenance and Regulation of Body Temperature......Page 392
35. Glucose Tolerance......Page 402
Appendix A: Preparation of Agar Plates and Pipettes......Page 414
Appendix B: Preparation of the Egg Osmometer......Page 415
Appendix C: Pithing the Frog......Page 416
Appendix D: Preparing the Freshwater Turtle for Heart Experiments......Page 417
Appendix E: Jones Pulmonor Disinfectant Procedure......Page 418
Index......Page 419
Medical and Pharmacological Abbreviations......Page 432