Enzymology and Molecular Biology of Carbonyl Metabolism 5

Front Matter....Pages i-xi
Site Directed Mutagenesis to Probe for Active Site Components of Liver Mitochondrial Aldehyde Dehydrogenase....Pages 1-7
Substrate Binding Pocket Structure of Human Aldehyde Dehydrogenases....Pages 9-16
Human Class 1 Aldehyde Dehydrogenase....Pages 17-23
Nitrate Esters as Inhibitors and Substrates of Aldehyde Dehydrogenase....Pages 25-34
Use of a Chromophoric Reporter Group to Probe the Active Site of Cytosolic Aldehyde Dehydrogenase....Pages 35-43
Studies of the Esterase Activity of Cytosolic Aldehyde Dehydrogenase Using Sterically Hindered and Cyclic Substrates....Pages 45-52
The Reduction of Propionic Anhydride by Aldehyde Dehydrogenase-NADH Mixtures at pH 7....Pages 53-60
Cloning and Characterisation of the cDNA for Sheep Liver Cytosolic Aldehyde Dehydrogenase....Pages 61-66
Crystallization of Sheep Liver Cytosolic Aldehyde Dehydrogenase in a form Suitable for High Resolution X-Ray Structural Analysis....Pages 67-70
Progress Toward the Tertiary Structure of (Class 3) Aldehyde Dehydrogenase....Pages 71-77
UDP-Glucose Dehydrogenase....Pages 79-84
Kinetic Studies on Class 3 Aldehyde Dehydrogenase from Bovine Cornea....Pages 85-92
Covalent Modification of Class 2 and Class 3 Aldehyde Dehydrogenase by 4-Hydroxynonenal....Pages 93-101
Constitutive and Overexpressed Human Cytosolic Class-3 Aldehyde Dehydrogenases in Normal and Neoplastic Cells/Secretions....Pages 103-114
Metabolism of Cyclophosphamide by Aldehyde Dehydrogenases....Pages 115-122
Tissue-Specific Expression and Preliminary Functional Analysis of the 5’ Flanking Regions of the Human Mitochondrial Aldehyde Dehydrogenase ( ALDH2 ) Gene....Pages 123-130
Transgenesis of the Aldehyde Dehydrogenase-2 ( ALDH2 ) Locus in a Mouse Model and in Cultured Human Cells....Pages 131-136
Class 3 Aldehyde Dehydrogenase....Pages 137-141
Studies on the Induction of Rat Class 3 Aldehyde Dehydrogenase....Pages 143-149
Mouse Class 3 Aldehyde Dehydrogenases....Pages 151-158
Cloning and Characterization of Genes Encoding Four Additional Human Aldehyde Dehydrogenase Isozymes....Pages 159-168
New Human Aldehyde Dehydrogenases....Pages 169-172
Retinoic Acid Synthesizing Enzymes in the Embryonic and Adult Vertebrate....Pages 173-183
Retinoic Acid Synthesis in the Developing Spinal Cord....Pages 185-192
Structure and Mechanism of Aldehyde Reductase....Pages 193-201
Expression of Human and Rat Carbonyl Reductase in E. Coli....Pages 203-209
Molecular Cloning and Sequencing of Mouse Hepatic 11ß-Hydroxysteroid Dehydrogenase/Carbonyl Reductase....Pages 211-221
Molecular Modelling Calculations on the Binding of D- and L-Xylose to Wild-Type Aldose Reductase and its H110Q and H110A Mutants....Pages 223-227
Stopped-Flow Studies of Human Aldose Reductase Reveal which Enzyme form Predominates During Steady-State Turnover in Either Reaction Direction....Pages 229-240
Lysine Residues in the Coenzyme-Binding Region of Mouse Lung Carbonyl Reductase....Pages 241-248
Substrate Specificity and Kinetic Mechanism of Tetrahymena 20α-Hydroxysteroid Dehydrogenase....Pages 249-258
Purification and Characterization of Recombinant Human Placental and Rat Lens Aldose Reductases Expressed in Escherichia Coli....Pages 259-268
Rat and Human Bile Acid Binders are Members of the Monomeric Reductase Gene Family....Pages 269-280
The Alcohol Dehydrogenase System....Pages 281-294
Promoters of the Mammalian Class III Alcohol Dehydrogenase Genes....Pages 295-300
Class I and Class IV Alcohol Dehydrogenase (Retinol Dehydrogenase) Gene Expression in Mouse Embryos....Pages 301-313
Molecular Evolution of Class I Alcohol Dehydrogenases in Primates....Pages 315-320
The Role of Leucine 116 in Determining Substrate Specificity in Human B 1 Alcohol Dehydrogenase....Pages 321-325
Mutations of Human Class III Alcohol Dehydrogenase....Pages 327-330
Human and Rat Class IV Alcohol Dehydrogenases....Pages 331-339
Cloning and Expression of a Human Stomach Alcohol Dehydrogenase Isozyme....Pages 341-347
Purification and Properties of Murine Corneal Alcohol Dehydrogenase....Pages 349-354
Mammalian Class VI Alcohol Dehydrogenase....Pages 355-364
Crystallizations of Novel Forms of Alcohol Dehydrogenase....Pages 365-371
Human Sorbitol Dehydrogenase Gene....Pages 373-381
Short-Chain Dehydrogenases/Reductases....Pages 383-395
Zinc Binding of Alcohol and Sorbitol Dehydrogenases....Pages 397-406
Horse Liver Alcohol Dehydrogenase-Catalyzed Aldehyde Oxidation....Pages 407-415
Analytical Approaches to Alcohol Dehydrogenase Structures....Pages 417-426
Purification and Characterization of S -Formylglutathione Hydrolase from Human, Rat and Fish Tissues....Pages 427-433
Useful Mutants of Zymomonas Mobilis Alcohol Dehydrogenase-2 Obtained by the Use of Polymerase Chain Reaction Random Mutagenesis....Pages 435-440
Back Matter....Pages 441-442