Enzymes of Lipid Metabolism

Front Matter....Pages i-xiv
Enzymes Metabolizing Phospholipids: From Infancy to Middle Age....Pages 1-13
Front Matter....Pages 15-15
Fatty Acid Biosynthesis during Brain Development....Pages 17-26
The Role of Soluble Acyl-Thioester Hydrolase in Fatty Acid Chain-Length Termination in Rabbit Mammary Gland and Liver....Pages 27-36
Stereochemical Studies of Hydrogen Incorporation from Nucleotides with Fatty Acid Synthetase from Brevibacterium ammoniagenes ....Pages 37-43
Cholesterol Oxidase as a Probe for Studying Membrane Composition and Organization....Pages 45-56
Adsorption and Activation of Pancreatic Lipase at Interfaces....Pages 57-68
Mode of Action of Pancreatic Colipase....Pages 69-78
Studies of Lipase and Phospholipase A 2 Acting on Lipid Monolayers....Pages 79-94
Inhibition of Lipase Adsorption at Interfaces. Role of Bile Salts Micelles and Colipase....Pages 95-100
Lipases of Rat Brain Microsomes....Pages 101-111
Identification and Some Characteristics of the Enzyme Protein of the Hormone-Sensitive Lipase from Rat Adipose Tissue....Pages 113-126
Affinity Chromatography on Heparin-Sepharose of Rat Adipose Tissue Triglyceride Lipase from Cytosol....Pages 127-134
Front Matter....Pages 135-135
Relationship between Structure and Activity of Pancreatic Phospholipase A 2 ....Pages 137-152
Kinetic Anomalies Associated with Phospholipase A 2 Hydrolysis of Micellar Substrates....Pages 153-163
Mechanism of Phospholipase A 2 Action Toward Mixed Micelles of Detergent and Phospholipids....Pages 165-175
Chemical Modification of Crotalus Atrox Phospholipase A 2 by Means of a Photolabile Phosphatidylethanolamine Analog....Pages 177-183
Phospholipases a of Krebs II Ascites Cells: Specificity, Release and Subcellular Localization....Pages 185-190
Phospholipases of Plasmic Membranes of Adipose Tissue. Possible Intermediaries for Insulin Action.....Pages 191-199
Calcium-Activated Hydrolysis of Triphosphoinositide in Synaptosomes....Pages 201-212
The Activity of Phospholipase D on Aggregates of Phosphatidylcholine, Dodecylsulfate and Ca 2+ ....Pages 213-220
Front Matter....Pages 135-135
The Preparation of Phospholipids by Phospholipase D....Pages 221-226
The Effects of Amphiphilic Compounds on Phosphatidate Metabolism....Pages 227-234
Choline and Ethanolamine Kinase Activity in the Cytoplasm of Nerve Endings from Rat Forebrain....Pages 237-245
The Release of Free Ethanolamine in Rat Brain Homogenates Incubated in Krebs Ringer....Pages 247-251
Ethanolamine and Choline Phosphotransferases of Chicken Brain....Pages 253-268
Studies of Rat Brain Choline Ethanolamine Phosphotransferases Using Labeled Alkylacylglycerol as Substrate with Evidence for Reversibility of the Reactions....Pages 269-278
The Role of Phosphatidylglycerol as a Donor of Phosphatidyl and of sn -Glycerol-1-Phosphate Groups in Biosynthetic Reactions....Pages 279-285
Membrane-Bound Base-Exchange Reactions in Animal Tissues....Pages 287-299
The “Base-Exchange” Reaction : The Serine Enzyme....Pages 301-318
A Study on the Possible Occurrence of Base-Exchange Reactions in Vivo ....Pages 319-325
The Biosynthesis and Hormonal Regulation of Surfactant Formation....Pages 327-339
Properties of a Lysophospholipase-Transacylase from Rat Lung: Its Possible Involvement in the Synthesis of Lung Surfactant....Pages 341-351
A Study on the Turnover of Rat Brain Phosphatidic Acid through the Glycerol-Phosphate Pathway....Pages 353-366
Studies on the Biosynthesis of the O-Alkyl Bond in Glycerol Ether Lipids....Pages 369-378
Asymmetric Localization of Alkyldihydroxyacetone-P Synthase and Acyldihydroxyacetone-P Acyltransferase in Microsomal Vesicles....Pages 379-385
Studies on Soluble Proteins Stimulating Plasmalogen Biosynthesis....Pages 387-395
Pathway for Hydrolysis of Plasmalogens in Brain....Pages 397-406
Turnover of Molecular Species of Diacyl and Alkyl Ether Phospholipids in Ehrlich Ascites Tumor Cells....Pages 407-413
Front Matter....Pages 415-415
Neuraminidase Gangliosides Interactions....Pages 417-437
Action of Rat Brain Sialidase on Synaptic Membrane Components in Situ ....Pages 439-446
Front Matter....Pages 415-415
Interaction of Human Brain Neuraminidase with Tritium—Labelled Gangliosides....Pages 447-462
Studies on Bovine Brain Membrane-Bound Neuraminidase (Sialidase)....Pages 463-474
Intraneuronal Distribution of Cytosoluble Neuraminidase in Pig Brain....Pages 475-483
Effect of Detergents on the Utilization of Sphingomyelin by Brain Sphingomyelinases....Pages 487-500
Sphingomyelinase of Human Tissues....Pages 501-511
Purification and Partial Characterization of Human Liver α-Galacto-Sidase: Is α-Galactosidase B an α- N -Acetylgalactosaminidase?....Pages 515-523
The Identity of α-Galactosidase B from Liver....Pages 525-529
Effect of Detergents on Ceramide-3 Hydrolysis by α-Galactosidase A....Pages 531-535
Specificity of Some Exo- and Endo-ß-Galactosidases Toward Glycosphingolipids....Pages 537-544
Studies of Activity and Stability of Immobilized ß -N-Acetylhexosaminidase....Pages 545-554
Affinity Methods for the Purification of Arylsulphatase A....Pages 555-560
Existence, Localization and Some Properties of the Activators of Sphingolipid Hydrolases....Pages 561-571
Studies on the Function of the Activator of Sulphatase A....Pages 573-582
Glucosidase and Glucocerebrosidase Activity in Subcellular Fractions of Rat Spleen....Pages 583-588
Inhibitors of β-Glucosidases of Animal Tissues....Pages 589-600
Isolation of Lysosomes from Bovine Brain Tissue a New Zonal Centrifugation Technique....Pages 601-610
UPD-Galactose: Ceramide Galactosyltransferase. Kinetic Properties and Effect of Detergents and Phospholipids on the Partially Purified Enzyme of Rat Brain....Pages 613-630
Isolation of an Endogenous Inhibitor of Ceramide Glycosyltransferases from Rat Brain....Pages 631-639
Cellular Localization of Na + K + -Dependent ATpase and of the Enzymes of Sulpholipid Metabolism in Rabbit Kidney....Pages 641-648
Control of Ganglioside Biosynthesis by a Rate Limiting UMP-ASE and Product Ganglioside Inhibition....Pages 649-654
Front Matter....Pages 415-415
Studies on Retinal Ganglioside Metabolism....Pages 655-665
Characteristics of the Brain Tissue Disialoganglioside G 2a ....Pages 667-678
Formation of Glycosylated “Proteolipid-Like” Materials during Exogenous Protein Galactosylation by Human Body Fluids....Pages 679-686
Front Matter....Pages 687-687
Diagnoses of the Sphingolipidoses with Labelled Natural Substrates....Pages 689-706
Assay of ß-Glucosidase and Sphingomyelinase for Identification of Patients and Carriers of Gaucher’s and Niemann-Pick Diseases....Pages 707-717
Hair Root Analysis in Heterozygotes for Fabry’s Disease....Pages 719-725
Substrate Specificity of Hexosaminidase a Isolated from the Liver of a Patient with a Rare Form (AB Variant) of Infantile G M2 Gangliosidosis and Control Tissues....Pages 727-730
Biochemical Studies in Sphingomyelin Storage Disorders....Pages 731-743
Factors that Influence the Uptake and Turnover of Glucocerebrosidase and α-Galactosidase in Mammalian Liver....Pages 745-752
Enzyme Therapy XI: Immunologic Considerations for Replacement Therapy with Unentrapped, Erythrocyte- and Liposome-Entrapped Enzymes....Pages 753-764
Back Matter....Pages 765-791