Enzyme Functionality Design Engineering And Screening.Svendsen

Preface......Page 4
Contents......Page 10
Contributors......Page 14
Concepts for Protein Engineering......Page 24
Sequence Families and Modular Organization of Carbohydrate-Active Enzymes*......Page 38
Analyzing Three-Dimensional Structures of Variant Enzymes......Page 58
Quantitative Modeling of Lipase Enantioselectivity......Page 82
Rational Redesign of Haloalkane Dehalogenases Guided by Comparative Binding Energy Analysis......Page 102
Computer Simulations: A Tool for Investigating the Function of Complex Biological Macromolecules......Page 120
Calculations of Ionization Equilibria in Proteins......Page 172
Modeling and Optimization of Directed Evolution Protocols......Page 208
Rational Redesign of Enzymes......Page 236
Details in the Reaction Mechanism of Chitinases......Page 252
Kinetic Evolution to the Catalytic Core of the Bacterial Phosphotriesterase......Page 270
Protein Engineering of PQQ Glucose Dehydrogenase......Page 284
The Proline Rule: A Concept for Engineering Protein Stability......Page 316
Homing Endonucleases: Tools and Targets for Protein Engineering......Page 348
Evolutionary Methods for Protein Engineering......Page 376
Directed Evolution by Random Mutagenesis: A Critical Evaluation......Page 398
Enzyme Engineering by Phage Display......Page 414
In Vivo Gene Shuffling in Yeast: A Fast and Easy Method for Directed Evolution of Enzymes......Page 436
Effective DNA Shuffling Methods for Enzyme Evolution......Page 448
Exploring the Functional Space of Combinatorial Mutant Libraries for the Directed Evolution of Novel Enzyme Activities......Page 466
Modifying the Character of an Enzyme by Producing Chimeric Enzymes: Chimeric &bgr;-glucosidases as an Illustration......Page 484
Assay Systems for Screening or Selection of Biocatalysts......Page 498
Screening of Enzyme Variants for Thermostability......Page 514
Combinatorial Mutagenesis Algorithms, Digital Imaging Spectroscopy, and Solid-Phase Assays for Directed Evolution......Page 530
Screen Automation and Robotics......Page 548
Screening for Enantioselective Enzymes......Page 582
Enzyme Engineering by Microbial Cell Surface Display......Page 622
Overexpression and Secretion of Biocatalysts in Pseudomonas......Page 640
Analysis of Catalytic and Structural Stability of Native and Covalently Modified Enzymes......Page 656
Index......Page 684