Enzyme Engineering: Volume 6

Front Matter....Pages i-xxii
Front Matter....Pages 1-1
The First Ten Years....Pages 3-7
Front Matter....Pages 9-9
Recent Studies on Antibiotics and Low Molecular Weight Enzyme Inhibitors....Pages 11-33
Front Matter....Pages 35-35
Integration of Large Scale Production and Purification of Biomolecules....Pages 37-43
Role of Active Transport of Inducer in Enzyme Biosynthesis and Process Scale-Up....Pages 45-59
Multi Enzyme Systems in Membrane Reactors....Pages 61-67
Scale-Up of Protein Purification by Liquid-Liquid Extraction....Pages 69-74
Rapid and Large-Scale Purification of Angiotensin-I Forming Enzymes and Milk Clotting Enzymes by Affinity Chromatography....Pages 75-76
Separation and Purification of Enzymes via Continuous PH-Parametric Pumping....Pages 77-78
Front Matter....Pages 79-79
Enzymatic Synthesis of Penicillins and Cephalosporins by Penicillin Acylase....Pages 81-90
Enzymatic Acyl Transfer in Penicillin and Cephalosporin Chemistry....Pages 91-96
Enzymatic Processes for the Synthesis of Optically Active Amino Acids....Pages 97-106
Horse Liver Alcohol Dehydrogenase: An Illustrative Example of the Potential of Enzymes in Organic Synthesis....Pages 107-116
Immobilized Enzymes and Synzymes: Applications in Organic Synthesis....Pages 117-122
Immobilization of Living Microbial Cells and Transformation of Steroids....Pages 123-124
Steroid Bioconversion by Immobilized Cells....Pages 125-126
SteroidΔ1-Dehydrogenase Isoenzymes in Corynebacterium Species Chol 73 T 191....Pages 127-128
Use of Immobilized Enzyme Requiring Cofactor Regeneration and of Immobilized Mycelium for Steroid Modification....Pages 129-130
Hydroxylation of Steroids by Immobilized Microbial Cells....Pages 131-133
Enzymatic Synthesis of Sulfur and Selenium Amino Acids....Pages 135-136
Production of Alkaloids with Immobilized Cells of Catharanthus Roseus ....Pages 137-138
Front Matter....Pages 79-79
Propyleneoxide Production by Immobilized Nocardia Corallina B-276....Pages 139-140
Air-Oxidation of Linoleic Acid by Lipoxygenase-Containing Particles Suspended in Water-Insoluble Organic Solvent....Pages 141-142
Progress Toward Artificial Photosynthesis....Pages 143-144
Light-Sensitization of a Microbial Protease....Pages 145-147
Enzymatic Photosensitive Materials....Pages 149-150
Ester Exchange of Triglyceride by Entrapped Lipase in Organic Solvent....Pages 151-152
Solvent Production by Clostridium Acetobutylicum in Aqueous Two-Phase Systems....Pages 153-155
Enzyme Kinetics and Mass-Transfer on Two Liquid Phase Heterogeneous Systems....Pages 157-158
Physico-Chemical Means of Increasing the Yield of End Products in Biocatalysis....Pages 159-160
Heat-Stable L-Lactate Dehydrogenase and its Application to an Enzyme Reactor....Pages 161-162
Multienzymes and Cofactors Immobilized within Lipid-Polyamide Membrane Microcapsules for Sequential Substrate Conversion....Pages 163-164
Tyrosine and 3,4-Dihydroxyphenyl-alanine Synthesis by Citrobacter freundii ....Pages 165-166
Enzyme Catalysts for the Stereo and Regio Selective Oxyfunctionalization of Organic Substrates....Pages 167-170
Front Matter....Pages 171-171
High-Rate, Continuous Waste Processor for the Production of High BTU Gas using Immobilized Microbes....Pages 173-180
New Developments in the Preparation and Characterization of Polymerbound Biocatalysts....Pages 181-189
Bioconversion of Lipophilic or Water Insoluble Compounds by Immobilized Biocatalysts in Organic Solvent Systems....Pages 191-200
Multiple Carrier Regeneration and Enzyme Immobilization in Situ ....Pages 201-202
Viability and Biosynthetic Capacity of Immobilized Plant Cells....Pages 203-204
Immobilized Enzymes of Heterogeneous Structure....Pages 205-206
Fibrous Support for Immobilization of Enzymes....Pages 207-208
Front Matter....Pages 171-171
High Temperature Cell-Trapped Ultrafiltration Membranes....Pages 209-210
Immobilized Modifiers for Proteins (IMPs)....Pages 211-212
Enzyme Immobilization in Porous Supports....Pages 213-214
Immobilization of Microbial Cells using Gelatin and Glutaraldehyde....Pages 215-216
Immobilization of β-Galactosidase from E. Coli CSH36 and its Microbial Cells Using Cellulose Beads....Pages 217-218
Hydrophobic Immobilization of Enzymes and Polynucleotides on Trityl Agarose....Pages 219-221
Influence of the Activation Degree of the Support on the Properties of Agarose-Nuclease....Pages 223-224
Immobilization of Biofunctional Components by Radiation Polymerization and Applications....Pages 225-226
Immobilization of Whole Cell Glucose Isomerase within Soybean Protein....Pages 227-228
Enzyme Stabilization in High Macromolecular Concentration Environments....Pages 229-231
Intrinsic Stability of Thermophilic Enzymes: 6-Phosphogluconate Dehydrogenase from Bacillus Stearothermophilus and Yeast....Pages 233-234
Stabilization of Penicillin Amidohydrolase Immobilized on Eupergit C....Pages 235-236
Stabilization of Fumarase Activity of Brevibacterium Flavum Cells by Immobilization with κ-Carrageenan and Polyethyleneimine....Pages 237-238
Application of Polyethylene Glycol-Bound NAD Derivative and Thermostable Dehydrogenase in a Model Enzyme Reactor....Pages 239-240
Use of the Porous Mineral Spherosil as a Carrier for Enzymes: Fixation and Purification....Pages 241-241
Front Matter....Pages 243-243
Potential Application of Immobilized Viable Cells in the Food Industry: Malolactic Fermentation of Wine....Pages 245-250
Production of L-Tryptophan with Immobilized Cells....Pages 251-257
Applications of Immobilized Tannin for Protein and Metal Adsorption....Pages 259-264
Industrial Applications of Immobilized Biomaterials in China....Pages 265-270
Production of L-Alanine from Ammonium Fumarate Using Two Types of Immobilized Microbial Cells....Pages 271-272
Front Matter....Pages 243-243
New Process for Production of High Fructose Corn Syrup Using Combined Adsorption and an Enzyme Reactor....Pages 273-274
Immobilized Multienzyme Systems for Starch Processing....Pages 275-277
Glucoamylase Covalently Coupled to Porous Glass....Pages 279-280
Rotary Multidisc Reactor of Collagen Supported Immobilized Glucoamylase....Pages 281-282
Kinetics for the Hydrolysis of Soluble Starch by Glucoamylase and Application to an Immobilized Enzyme System....Pages 283-285
Reduced Thermostability of Modified Mucor Miehei Rennet....Pages 287-288
Application of Immobilized Enzymes to Milk Curdling....Pages 289-290
Production of 7-Aminodesacetoxycephalosporanic Acid by Immobilized E. Coli Cells....Pages 291-292
Technical Applications of Lactase and Aminoacid Acylase Immobilized to form Plexazym....Pages 293-294
Continuous Hydrolysis of Lactose in Skim Milk and Acid Whey by Immobilized Lactase of Aspergillus Oryzae ....Pages 295-297
Lactic Acid Fermentation with Immobilized Lactobacillus Sp.....Pages 299-301
Production of 1-Malic Acid with Immobilized Thermophilic Bacterium, Thermus Rubens Nov. sp.....Pages 303-304
Continuous Hydrolysis of Concentrated Sucrose Solutions by Immobilized Invertase....Pages 305-306
A Series of Covalently Bonded Enzymes and Their Applications....Pages 307-308
The Application of Fluidized Beds for Improved Enzyme Reactor Performance....Pages 309-310
Control of Continuous Coenzyme Regeneration....Pages 311-312
A New Approach to Membrane Reactor Design and Operation....Pages 313-314
Influence of Compaction in Gel-Immobilized Enzyme Packed Bed Reactors....Pages 315-316
Front Matter....Pages 317-317
Enzymatic Removal of Hazardous Pollutants from Industrial Aqueous Effluents....Pages 319-324
Enzymatic Hydrolysis of Cellulose: Effects of Structural Properties of Cellulose on Hydrolysis Kinetics....Pages 325-333
Front Matter....Pages 317-317
Continuous Ethanol Fermentation by Immobilized Biocatalysts....Pages 335-342
An Immobilized Yeast Cell Column for the Fermentation of Mosasses....Pages 343-345
Pilot Operation for Continuous Alcohol Fermentation of Molasses in an Immobilized Bioreactor....Pages 347-348
Enzymatic Hydrolysis of Cellulose....Pages 349-350
ATP Regeneration by Enzymes of Alcohol Fermentation and Kinases of Yeast and its Computer Simulation....Pages 351-352
Extracellular Cellulases Produced by a Yeast-Like Fungus....Pages 353-354
Relationship between Extracellular Proteases and the Cellulase Complex of Trichoderma Reesei ....Pages 355-356
Front Matter....Pages 357-357
Binding Assays Involving Separation in Aqueous Two-Phase Systems: Partition Affinity Ligand Assay (PALA)....Pages 359-367
Use of Immobilized Enzyme Reactors in Automated Clinical Anlayses....Pages 369-375
Immobilized Enzymes in Analysis: Applications and Economic Aspects....Pages 377-386
Microbial Sensors for Gas Analysis....Pages 387-393
Analytical Uses of Immobilized Enzymes....Pages 395-404
Cost Analysis and Viability of Immobilized Enzymes in Routine Analysis....Pages 405-407
Multipurpose Enzyme-Collagen Membrane Electrodes....Pages 409-410
Long-Term Stability of Air-Dried Enzyme Electrodes with Selective Enzymic Collagen Membranes....Pages 411-412
Enzyme Electrodes Based on Insolubilized Enzyme Membranes Coupled with an Electrochemical Transducer....Pages 413-414
Potentiometric Glucose Sensor: Enzymatic Activity and Potentiometric Measurements....Pages 415-416
Microbial Sensor for Preliminary Screening of Mutagens....Pages 417-418
Application of Microbial Electrode to Analysis of Waste Water....Pages 419-420
Biosensors Based on Enzyme Amplification and Immunochemical Selectivity....Pages 421-422
Front Matter....Pages 357-357
Application of Chemiluminescence of Cypridina Luciferin Analog to Immobilized Enzyme Sensors....Pages 423-424
Use of Hydrogen Sensitive Pd-MOS Components in Biochemical Analysis....Pages 425-427
Computer Controlled Mass Spectrometer Monitoring of Fermentations....Pages 429-430
Front Matter....Pages 431-431
Immobilized Heparinase: Production, Purification, and Application in Extracorporeal Therapy....Pages 433-441
Clinical Utility of Urokinase-Treated Polymer for Antithrombogenic Material....Pages 443-449
Artificial Cell Immobilized Multienzyme Systems and Cofactors....Pages 451-456
Antithrombogenic Activity of Artificial Medical Materials Improved by Enzyme Immobilization Techniques....Pages 457-458
Application of Immobilized Enzymes for Biomaterials used in the Field of Thoracic Surgery....Pages 459-460
Use of Immobilization Principles for the Construction of Drug Targeting Systems....Pages 461-463
Application of Bioreactors with Immobilized L-Asparaginase....Pages 465-466
Acyl-CoA Synthetase and Acyl-CoA Oxidase for Determination of Serum Free Fatty Acids....Pages 467-468
Enzyme Immunoassay for Free Thyroxine....Pages 469-472
Routine Determination of Hydrogen Peroxide in Clinical Chemistry with Immobilized Aldehyde Dehydrogenase....Pages 473-474
Enzyme Electrodes for Simultaneous Determination of Creatinine and Creatine in Serum or Whole Blood....Pages 475-476
Front Matter....Pages 477-477
Construction of Various Host Vector Systems and the Variation of Enzyme Levels....Pages 479-489
Enzymes Active on Unnatural Synthetic Compounds: Nylon Oligomer Hydrolases Controlled by a Plasmid and their Cloning....Pages 491-500
Front Matter....Pages 501-501
Recent Developments and Future Aspects of Enzyme Engineering....Pages 503-510
Back Matter....Pages 511-538