Enzyme Engineering: Volume 5

Front Matter....Pages i-xviii
Front Matter....Pages 1-1
Achievements and Predicted Developments in Enzyme Engineering....Pages 3-29
Front Matter....Pages 31-31
Comparative Quantitative Physiology of High Cellulase Producing Strains of Trichoderma Reesei ....Pages 33-40
Bacterial Exo-Enzyme and Exo-Toxin Export....Pages 41-44
Enzyme Purification by Liquid-Liquid Extraction....Pages 45-47
Continuous Isolation of Yeast-Lytic Enzymes from Citophaga....Pages 49-55
Characterization of Membranes for Enzyme Retention....Pages 57-59
Cloning of the Penicillin G-Acylase Gene of Escherichia Coli ATCC 11105 on Multicopy Plasmids....Pages 61-69
Immobilized Tannin as Protein Adsorbent-Preparation, Characteristics, and Applications....Pages 71-73
Aminoalkylpolysaccharide Sorbents in Purification of Enzymes....Pages 75-77
Immunochemical Identification of a New Thermostable β-Galactosidase from a Bacillus Species....Pages 79-83
Mass Spectrometer Monitoring of a Yeast Fermentation....Pages 85-88
Affinity Chromatographic Investigations on Enzyme Coenzyme Complexes of CoA-Dependent Enzymes....Pages 89-92
Front Matter....Pages 93-93
Principles of Bioelectrocatalysis....Pages 95-100
Cofactor Modified Electrodes for Energy Transfer....Pages 101-108
Immobilized Thylakoids and Chromatophores: Hydrogen Production and ATP Regeneration....Pages 109-118
Some Aspects of Direct Bioelectrocatalytic Regeneration of Cofactors....Pages 119-121
A New Enzyme Transducer Combination: The Enzyme Transistor....Pages 123-126
A Simple, General Method for Preparation of Hydrosoluble Polymeric Adenine Nucleotide Coenzymes....Pages 127-131
Cofactor Reaction-Diffusion Kinetics for an Alcohol Dehydrogenase Membrane....Pages 133-134
Approaches to Stabilization of Hydrogenase and Nitrogenase Against Oxygen Inactivation....Pages 135-142
Front Matter....Pages 93-93
Biochemical Energy Conversion System....Pages 143-145
Front Matter....Pages 147-147
Utilization of β-Glucosidase from Aspergillus Species in the Hydrolysis of Cellulose....Pages 149-151
The Comparative Role of Exoglucosidase and Cellobiase in Glucose Formation from Cellulose....Pages 153-165
Novel Enzymes from Methylotrophic Microorganisms....Pages 167-169
Ethanol — Butanediol Fermentation of Xylose....Pages 171-173
Fluorimetric Monitoring of Methanogenesis in Anaerobic Digesters....Pages 175-176
Front Matter....Pages 177-177
Enzyme Channelling Immunoassay. A New Homogeneous Enzyme Immunoassay Technique....Pages 179-186
Human Renal Renin: Purification, Production of Specific Antibodies. Clinical Applications....Pages 187-191
Competitive Binding Studies of Carbohydrate-Lectin Interactions Using a Lectin Electrode....Pages 193-196
Potentiometric Enzymatic Measurement of Glucose: Possible in vivo Use....Pages 197-199
Immobilized Enzyme Pipette — ‘Impette’....Pages 201-204
Electrochemical Determination of Lipids in Serum....Pages 205-207
Immobilized Enzymes for Medical Application....Pages 209-211
Photoenzography: Using Enzymes for Photographic Aims....Pages 213-216
The Design of Peroxide Enzyme Membrane Polarographic Sensors for Clinical and Industrial Analysis....Pages 217-223
New Approaches Using Immobilized Enzymes for the Removal of Urea and Ammonia....Pages 225-229
Enzyme-Collagen Reactor Designed from an Hemodialysis Module....Pages 231-233
Immobilization and Characterization of Enzymes on Hollow Fibers for a Possible Use in the Biomedical Field....Pages 235-237
Magnetic Microspheres for Targeting of Drugs....Pages 239-241
Modification of Enzymes with Water-Soluble Polymers....Pages 243-245
Front Matter....Pages 177-177
Detoxification of Ammonia by Immobilized Urea Cycle Enzymes....Pages 247-250
Use of the Enzyme Thermistor for Continuous Monitoring and Control in Biotechnology....Pages 251-254
Distribution of Damkohler Number of Spherical Matrix Particles by Image Analysis....Pages 255-258
On-line Determination of Reaction Rate Versus Substrate Concentration for an Enzymatically Catalysed Reaction by Means of a Microcomputer System....Pages 259-261
Microbial Sensors for Ethyl Alcohol, Acetic Acid and Ammonia....Pages 263-265
Front Matter....Pages 267-267
Improved Whey Treatment by Immobilized Lactase....Pages 269-278
Lactose Hydrolysis by Immobilized Lactase: Semi-Industrial Experience....Pages 279-293
Production of Useful Nucleotides with Immobilized Microbial Cells....Pages 295-304
Heterogeneous Biocatalysis in the Degradation of β-Glucan Polymers....Pages 305-308
Absorption Into a Liquid Film in Laminar Flow Coupled with an Enzyme Catalyzed Reaction At the Solid Surface....Pages 309-315
Catalyst Deactivation and Optimal Operation Policy: Immobilized β-Galactosidase....Pages 317-320
Stability Studies on the Immobilized Glucose Oxidase/Catalase Enzyme System....Pages 321-324
Hydrodynamics and Kinetics of Hollow Fiber Enzyme Reactors....Pages 325-327
Isomerose Syrups Containing Greater than 90% Fructose....Pages 329-331
Front Matter....Pages 333-333
Immobilization of Whole Microbial Cells for the Production of 6- Amino Penicillanic Acid....Pages 335-345
Application of Immobilized Biocatalysts to Bioconversion in Hydrophobic Conditions....Pages 347-353
Alginate Bead Entrapped Yeast Cells for Continuous Inversion of Sucrose and Molasses....Pages 355-358
Physical Characterization of Biocatalyst Particles Obtained from Polymer Entrapment of Whole Cells....Pages 359-362
The use of Free and Immobilized Cells in the Presence of Organic Solvents: The Oxidation of Cholesterol by Nocardia Rhodochrous ....Pages 363-367
Properties of Enzymes Solubilized in Hydrocarbons Via Reversed Micelles....Pages 369-371
Front Matter....Pages 333-333
The Potential Use of Immobilized Plant Cells for the Production and Transformation of Natural Products....Pages 373-381
Immobilized Whole Cells of the Yeast Trigonopsis Variabilis Containing D-Amino Acid Oxidase For The Production of α-Keto Acids....Pages 383-387
Enzymatic Synthesis of Pantothenic Acid by Escherichia Coli Cells....Pages 389-391
Production of Useful Chemicals Using Cells Immobilized with Polyacrylamide and Carrageenan....Pages 393-400
Affinity Chromatographic Purification of Proteins Using Immobilized Cells....Pages 401-403
Synthesis of Coenzymes by Immobilized Cell System....Pages 405-411
Front Matter....Pages 413-413
Continuous Deacetylation of Cephalosporins....Pages 415-422
Thermostability of Soluble and Immobilized Subtilisins after Their Modification by Dextrans and Dextrins....Pages 423-426
Isolation and Immobilization of Porcine Ligandin with Glutathione Transferase Activity....Pages 427-429
Studies on the Stability of Soluble and Immobilized Alcohol Dehydrogenase from Yeast Mitochondria....Pages 431-434
Distribution of Staphylococcal Nuclease Insolubilized on Sepharose....Pages 435-438
Immobilized Penicillin Acylase for Production of 6-Apa From Penicillin-V....Pages 439-442
Preparation and Characterization of Metal Oxide-Coated Supports for Oxidase Enzymes....Pages 443-445
Enzyme Immobilization on Pyridine Containing Polymers....Pages 447-449
Cross-Linked Pectate....Pages 451-452
Immobilization of Biocatalysts Using Ultrafiltration Techniques....Pages 453-456
β-Galactosidase Immobilized on Benzoquinone-Activated Bead Cellulose and on Oxirane-Acrylic Beads....Pages 457-460
Application of Immobilized Carboxypeptidase Y for Deblocking in Peptide Synthesis....Pages 461-463
Adsorption and Distribution of Enzymes in Carriers....Pages 465-468
Application of Photosensitive Immobilized α-Chymotrypsin to Synthetic Reaction....Pages 469-471
Back Matter....Pages 473-485