Enzyme Engineering: Volume 3

Front Matter....Pages i-xiv
Front Matter....Pages 1-1
Innovation in Enzyme Engineering....Pages 3-8
Front Matter....Pages 9-9
The Immobilization of a Stable Esterase by Entrapment, Covalent Binding and Adsorption....Pages 11-18
Liquid-Membrane Hollow Fiber Enzyme Reactors....Pages 19-28
Synthesis of Organic Acids and Modification of Steroids by Immobilized Whole Microbial Cells....Pages 29-41
Immobilization of Enzymes by Reductive Alkylation....Pages 43-49
Development of Immobilized Urease for the Owens-Illinois Bun Analyzer....Pages 51-56
Immobilization of β-Tyrosinase Cells with Collagen....Pages 57-62
Stepwise Thermophotochemical Crosslinking for Enzyme Stabilization and Immobilization....Pages 63-70
Front Matter....Pages 71-71
Mass Transfer Problems with Immobilized Oxidases....Pages 73-78
Mass Transfer and Reaction with Microcapsules Containing Enzyme and Adsorbent....Pages 79-84
Enzymatic Regeneration of ATP from AMP and ADP: Kinetic Studies with the Coupled Enzyme System....Pages 85-91
Cofactor Regeneration in Artificial Enzyme Membranes: Potentialities for Analytical and Reactor Applications....Pages 93-99
Optimal Operations of a Batch Enzyme Reactor: Isomerization of D-Glucose to D-Fructose....Pages 101-114
Physicochemical Aspects of Immobilized Enzyme-Matrix Interactions....Pages 115-132
Coupled and Multienzyme-Nylon Tube Reactors....Pages 133-138
Kinetics and Stability of Immobilized Glucose Oxidase and Catalase....Pages 139-144
Kinetics of D-Glucose to D-Fructose Isomerization....Pages 145-153
Efficiency of Two Immobilized Enzymes Acting in Sequence....Pages 155-161
Photo-Control of Enzyme-Collagen Membrane Activity....Pages 163-166
Enzyme Immobilization in Collagen Films Adhered to Semi-Rigid Supports: The Enzymatic Sponge Reactor....Pages 167-177
Front Matter....Pages 71-71
Hollow Fiber Enzymatic Reactors: An Engineering Approach....Pages 179-190
Front Matter....Pages 191-191
Genetic Engineering with Nitrogen Fixation....Pages 193-205
The State of Enzyme Isolation Technology....Pages 207-215
Unit Operations of Enzyme Purification....Pages 217-223
Scale-Up of Enzyme Purification....Pages 225-233
Carbon Dioxide as a Reagent for Proteins....Pages 235-239
Application of Computers to Enzyme Recovery....Pages 241-248
Studies on Immobilized Trypsin in High Concentrations of Organic Solvents....Pages 249-254
A Tapered Fluidized Bed as a Bioreactor....Pages 255-261
Induction and Purification of Phenol Hydroxylase from Tricosporon Cutaneum ....Pages 263-271
Front Matter....Pages 273-273
Analysis of Ligand-Macromolecule Interactions by Quantitative Affinity Chromatography....Pages 275-282
Affinity Chromatography: Interferences and Their Prevention....Pages 283-289
Immobilized Cofactors and Cofactor Fragments in General Ligand Affinity Chromatography and as Active Cofactors....Pages 291-298
Purification of Dehydrogenases and Kinases by Affinity Chromatography....Pages 299-311
Factors Concerning the Effectiveness of Affinity Chromatography Using Immobilized Cofactors....Pages 313-320
Hydrophobic Chromatography and Its Potential Use in Biotechnology....Pages 321-329
Purification of Proteinases by Affinity Chromatography Techniques....Pages 331-336
Covalent Affinity Chromatography of Acetylcholinesterase....Pages 337-346
Salt-Stable Hydrophobic Versus Salt-Reversible Electrostatic Effects in Adsorptive Protein Binding....Pages 347-355
Strategy for Pure Hydrophobic Chromatography....Pages 357-362
Front Matter....Pages 273-273
High Turnover NAD Regeneration in the Coupled Dehydrogenase Conversion of Sorbitol to Fructose....Pages 363-369
Microencapsulated Multi-Enzyme Systems as Vehicles for the Cyclic Regeneration of Free and Immobilized Coenzymes....Pages 371-377
Front Matter....Pages 379-379
Analytical and Biomedical Applications of Immobilized Enzymes: A Review....Pages 381-390
Refixation of Solubilized and Purified Microsomal Enzymes: Towards an Extracorporal Detoxification in Liver Failure....Pages 391-396
Immobilized Enzymes in Tubes and Hollow Fibers for Clinical Applications....Pages 397-408
The Cholesterol Electrode: Use of the Polarographic Oxidase Anode with Multiple Enzymes....Pages 409-426
Medical Applications of Affinity Chromatography....Pages 427-429
Studies on the Thermal Enzyme Probe....Pages 431-436
Prospects for an Assay Technique Based on Volatile Enzyme Products....Pages 437-443
Enzymatic Catalysis of a Three Step Steroid Conversion....Pages 445-452
Enzyme Thermistor Assays of Cholesterol, Oxalic Acid, Glucose and Lactose in Standard Solutions and Biological Samples....Pages 453-460
Front Matter....Pages 461-461
Industrial Production of L-Malic Acid by Immobilized Microbial Cells....Pages 463-468
Production of 5’-Mononucleotides Using Immobilized 5’-Phosphodiesterase and 5’-AMP Deaminase....Pages 469-475
A New Method for the Production of Optically Active Aminoacids....Pages 477-481
Development of an Adsorbed Lactase Immobilized Enzyme System....Pages 483-496
Studies on the Isomerization of D-Glucose by Immobilized Glucose Isomerase....Pages 497-508
Macrokinetics and Reactor Design for the Industrial Application of Enzymes in L-Amino Acid Production....Pages 509-517
Recent Trends in Enzyme Engineering in Japan....Pages 519-524
Pilot Plant Production of Glucose from Starch with Soluble α-amylase and Immobilized Glucoamylase....Pages 525-530
Production of High-Fructose Syrup Using Glucoamylase and Glucose Isomerase Immobilized on Phenol-Formaldehyde Resin....Pages 531-536
Front Matter....Pages 461-461
Reduction of Lactose in Milk by Entrapped β-Galactosidase IV. Results of Long Term Experiments with a Pilot Plant....Pages 537-542
Use of α-Galactosidase, β-Galactosidase, Glucose Isomerase and Invertase in Hollow Fiber Reactors....Pages 543-549
Back Matter....Pages 551-580