Enzyme Engineering Volume 2

Front Matter....Pages i-xiv
Developments and Challenge of Enzyme Engineering....Pages 3-7
1973 Henniker Delphi Study....Pages 9-14
Front Matter....Pages 15-15
Stability of Enzymes from Thermophilic Microorganisms....Pages 17-21
Polypeptide Hormones from Tissue Culture....Pages 23-30
Design Consideration for Animal Cell Cultures as Sources of Enzymes....Pages 31-40
Front Matter....Pages 41-41
Recent Developments in Enzyme Isolation Processes....Pages 43-45
Protein Purification by Immunoadsorption....Pages 47-54
Purification of Urokinase by Affinity Chromatography....Pages 55-62
Derivatives of Controlled Pore Glass for Affinity Chromatography....Pages 63-64
Front Matter....Pages 65-65
Review of Recent Enzyme Immobilization Techniques....Pages 67-76
Encapsulation of Enzymes in Liquid Membrane Emulsions....Pages 77-82
An Organic Millieu in Immobilized Enzyme Synthesis and Catalysis....Pages 83-90
Immobilization of Enzymes on Phenol-Formaldehyde Resins....Pages 91-96
Derivatized Nylon: A New Support for the Immobilization of Enzymes....Pages 97-104
New Immobilization Techniques and Supports....Pages 105-114
Stabilization and Immobilization of Enzymes with Imidoesters....Pages 115-122
Complexation of Enzymes or Whole Cells with Collagen....Pages 123-129
Immobilized Enzymes Using Resinous Carriers....Pages 131-135
Covalent Coupling of Small Molecules and Proteins to Poly (2-Hydroxyethyl Methacrylate-Methacrylic Acid) Hydrogels....Pages 137-139
Front Matter....Pages 141-141
Theoretical and Practical Aspects of Immobilized Multi-Step Enzyme Systems....Pages 143-152
Front Matter....Pages 141-141
Enzyme Reactions for Preparative Scale Synthesis....Pages 153-159
Application of Circular Dichroism to the Study of Enzyme Polymer Conjugates....Pages 161-164
Fluorescence Investigation of Immobilized Enzymes....Pages 165-168
Effects of the Matrix Environment on Protein Structure....Pages 169-177
Electron Spin Resonance Investigations into the Interaction Between Proteins and Synthetic Polymers....Pages 179-180
Some Observations on the Behavior of an Immobilized Allosteric Enzyme: Phosphofructokinase....Pages 181-182
Front Matter....Pages 183-183
Nucleotide Affinity Labels....Pages 185-193
Continuous Processing with Cofactor Requiring Enzymes: Coenzyme Retention and Regeneration....Pages 195-202
NADH Coupled to Soluble Dextran: Synthesis and Properties....Pages 203-208
Enzymatic Regeneration of ATP from AMP and ADP Part I. Thermodynamics, Kinetics, and Process Development....Pages 209-216
Enzymatic Regeneration of ATP from AMP and ADP Part II : Enzyme Immobilization and Reactor Development....Pages 217-222
ATP Regeneration Using Immobilized Carbamyl Phosphokinase....Pages 223-228
Immobilization of Coenzyme A and its Application....Pages 229-236
Synthesis and Application of Matrix Bound AMP, NAD + and Other Adenine Nucleotides....Pages 237-242
Front Matter....Pages 243-243
Well-Mixed Immobilized Enzyme Reactors....Pages 245-251
Reactor Analysis with Laser Microspectrophotometry....Pages 253-258
Open Tubular Heterogeneous Enzyme Reactors....Pages 259-267
Annular Column Enzyme Reactors....Pages 269-270
Kinetic Analysis of a Urease Electrode....Pages 271-278
Temperature Effects on the Design of Enzyme Reactors....Pages 279-282
Front Matter....Pages 243-243
Immobilized Enzymes: Diffusion and Sigmoid Kinetics in Packed Bed Reactors....Pages 283-284
Front Matter....Pages 285-285
Food and Product Considerations in the Application of Immobilized Enzymes....Pages 287-292
Fiber Entrapped Enzymes....Pages 293-302
Continuous Enzyme Reactions by Immobilized Microbial Cells....Pages 303-313
Immobilized Glucose Isomerase for the Production of High Fructose Syrups....Pages 315-328
Treatment of Milk with Immobilized Proteases and Oxidoreductases....Pages 329-336
Removal of Serum Hepatitis Antigen from Factor IX with an Immunoadsorbent....Pages 337-338
Immobilized Lactase in Fluidized Bed Reactors for Treating Cheese Whey....Pages 339-340
Front Matter....Pages 341-341
Application of a Heat Stable Bacterial Amylase in the Starch Industry....Pages 343-350
Toxicologic Studies on Proteolytic Enzymes in Animals and Man....Pages 351-362
Recent Trends of Enzyme Engineering in Japan....Pages 363-367
Survey of the Enzyme Industry....Pages 369-373
Front Matter....Pages 375-375
Analytical Uses of Immobilized Enzymes....Pages 377-383
Application of Immobilized Enzymes to Chemical Analysis....Pages 385-392
Application of Immobilized Enzymes to Detection and Monitoring....Pages 393-400
Preparation of Immobilized Enzymes for Application in Automated Analysis....Pages 401-407
Initiation of Enzyme Reactions by Light....Pages 409-410
The Thermal Enzyme Probe — A Novel Approach to Chemical Analysis....Pages 411-417
Effects of Different Routes of In Vivo Administration of Microencapsulated Enzymes....Pages 419-424
L-Asparaginase from Escherichia Coli II and Erwinia Carotovora Bound to Poly(Methyl Methacrylate)....Pages 425-432
Front Matter....Pages 375-375
Trends in the Use of Immobilized Enzymes and Proteins in Human Therapeutics....Pages 433-438
Use of Collagen Immobilized Enzymes in Blood Treatment....Pages 439-445
Back Matter....Pages 447-470